Isolation of rainbow trout (Oncorhynchus mykiss) intestinal intraepithelial lymphocytes (IEL) and measurement of their cytotoxic activity

Fish and Shellfish Immunology

Volumn 7, Issue 8, 1997, Pages 527-541

  McMillan, D Nelson ^a   Secombes, Christopher J ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Cytotoxic activity; GALT; Intestinal intraepithelial lymphocytes; Rainbow trout

Indexed keywords

MYKISS; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 0031280710     PISSN: 10504648     EISSN: None     Source Type: Journal    
DOI: 10.1006/fsim.1997.0099     Document Type: Article

References (29)

1. Beagley, K. W., Fujihashi, K., Lagoo, A. S., Lagoo-Deenadaylan, S., Black, C. A., Murray, A. M., Sharmanov, A. T., Yamamoto, M., McGhee, J. R., Elson, C. O. & Kiyono, H. (1995). Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine. Journal of Immunology 154, 5611-5619.
2. Berke, G. (1995). Unlocking the secrets of CTL and NK cells. Immunology Today 16, 343-346.
3. Camenisch, T. D., Jaso-Friedmann, L., Evans, D. L. & Harris, D. T. (1993). Expression of a novel function-associated molecule on cells mediating spontaneous cytolysis in swine. Developmental and Comparative Immunology 17, 277-282.
4. Chai, J. Y. & Lillehoj, H. S. (1988). Isolation and functional characterization of chicken intestinal inter-epithelial lymphocytes showing natural killer cell activity against tumour target cells. Immunology 63, 111-117.
5. Deluca, D., Wilson, M. & Warr, G. W. (1983). Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM. European Journal of Immunology 13, 546-551.
6. Doggett, T. A. & Harris, J. E. (1991). Morphology of the gut associated lymphoid tissue of Oreochromis mossambicus and its role in antigen absorption. Fish & Shellfish Immunology 1, 213-227.
7. Ellis, A. E. (1995). Recent development in oral vaccination delivery systems. Fish Pathology 30, 293-300.
8. Evans, D. L. & Jaso-Friedmann, L. (1992). Nonspecific cytotoxic cells as effectors of immunity in fish. Annual Review of Fish Diseases 2, 109-121.
9. Evans, D. L., Carlson, R. L., Graves, S. S. & Hogan, K. T. (1984). Nonspecific cytotoxic cells in fish (Ictalurus punctatus). IV. Target cell binding and recycling capacity. Developmental and Comparative Immunology 8, 823-833.
10. Evans, D. L., Leary, J. H. & Jaso-Friedmann, L. (1995). Cross-linkage of a receptor on nonspecific cytotoxic cells or treatment with calcium ionophore triggers increased phosphorylation of serine residues. Journal of Fish Biology 47A, 76-84.
11. Georgopoulou, U. & Vernier, J.-M. (1986). Local immunological response in the posterior intestinal segment of the rainbow trout after oral administration of macromolecules. Developmental and Comparative Immunology 10, 529-537.
12. Guy-Grand, D. M., Malassis-Seris, M., Briottet, C. & Vassalli, P. (1991). Cytotoxic differentiation of mouse gut thymodependent and independent intraepithelial T lymphocytes is induced locally. Journal of Experimental Medicine 173, 1549-1555.
13. Hamad, M. & Klein, J. R. (1994). Phenotypic and functional heterogeneity of murine intestinal intraepithelial lymphocytes defined by cell density: implications for route of differentiation and responsiveness to proliferation induction. Immunology 82, 611-616.
14. Hayden, B. J. & Laux, D. C. (1985). Cell-mediated lysis of murine target cells by nonimmune salmonid lymphoid preparations. Developmental and Comparative Immunology 9, 627-639.
15. Jaso-Friedmann, L., Leary, J. H., Warren, J. & Evans, D. L. (1995). Peptide mapping of the epitope on target cells recognized by non-specific cytotoxic cells (NCC) from channel catfish. Journal of Fish Biology 47A, 95-102.
16. Korzeniewski, C. & Callewaert, D. M. (1983). An enzyme-release assay for natural cytotoxicity. Journal of Immunological Methods 64, 313-320.
17. Lester, J. P., Evans, D. L., Leary, J. H., Fowler, S. C. & Jaso-Friedmann, L. (1994). Identification of a target cell antigen recognised by nonspecific cytotoxic cells using an anti-idiotype monoclonal antibody. Developmental and Comparative Immunology 18, 219-229.
18. McGhee, J. R., Mestecky, J., Dertzbaugh, M. T., Eldridge, J. H., Hirasawa, M. & Kiyono, H. (1992). The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10, 75-88.
19. Moody, C. E., Serreze, D. V. & Reno, P. W. (1985). Non-specific cytotoxic activity of teleost leukocytes. Developmental and Comparative Immunology 9, 51-54.
20. Mosley, R. L. & Klein, J. R. (1992). A rapid method for isolating murine intestine intraepithelial lymphocytes with high yield and purity. Journal of Immunological Methods 156, 19-26.
21. Mowat, A. M. S., McInnes, I. B. & Parrott, D. M. V. (1986). Functional characteristics of intraepithelial lymphocytes from mouse small intestine. II. In vivo and in vitro responses of intraepithelial lymphocytes to mitogenic and allogeneic stimuli. Immunology 58, 627-634.
22. Nagi, A. M. & Babiuk, L. A. (1987). Bovine gut-associated lymphoid tissue - Morphological and functional studies. I. Isolation and characterization of leukocytes from the epithelium and lamina propria of bovine small intestine. Journal of Immunological Methods 105, 23-37.
23. Robijn, R. J., Logtenberg, T., Wiegman, L. J. J. M., van Berge Henegouwen, G. P., Houwen, R. W. & Kroningsberger, J. C. (1995). Intestinal T lymphocytes. Scandinavian Journal of Gastroenterology 30 (Suppl 212), 23-33.
24. Rocha, B., Vassilli, P. & Guy-Grand, D. (1992). The extrathymic T-cell development pathway. Immunology Today 13, 449-454.
25. Rombout, J. H. W. M., Taverne-Thiele, A. J. & Villena, M. I. (1993). The gut-associated lymphoid tissue (GALT) of carp (Cyprinus carpio L.): An immunocytochemical analysis. Developmental and Comparative Immunology 17, 55-66.
26. Sawyerr, A. M., Arranz, E. & Ferguson, A. (1993). Intestinal intraepithelial lymphocytes: whence, whither and wherefore? European Journal of Gastroenterology and Hepatology 5, 903-906.
27. Sydora, B. C., Mixter, P. F., Holcombe, H. R., Eghtesady, P., Williams, K., Amaral, M. C., Nel, A. & Kronenberg, M. (1993). Intestinal intraepithelial lymphocytes are activated and cytolytic but do not proliferate as well as other T cells in response to mitogenic signals. Journal of Immunology 150, 2179-2191.
28. Van der Heijden, P. J. & Stok, W. (1987). Improved method for the isolation of functionally active lymphoid cells from the murine intestine. Journal of Immunological Methods 103, 161-167.
29. Van der Heijden, P. J., Stok, W. & Bianchi, A. T. J. (1987). Contribution of immunoglobulin-secreting cells in the murine small intestine to the total ' background ' immunoglobulin production. Immunology 62, 551-555.