Transforming Growth Factor-β1 Is a Potent Inhibitor of Glutathione Synthesis in the Lung Epithelial Cell Line A549: Transcriptional Effect on the GSH Rate-limiting Enzyme γ-Glutamylcysteine Synthetase

American Journal of Respiratory Cell and Molecular Biology

Volumn 17, Issue 5, 1997, Pages 599-607

  Arsalane, Karim ^b   Dubois, Claire M ^b   Muanza, Thierry ^b   Bégin, Raymond ^b   Boudreau, François ^b   Asselin, Claude ^b   Cantin, André M ^a  

^a CENTRE HOSPITALIER UNIVERSITAIRE DE SHERBROOKE   (Canada)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIA;

GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; RNA; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; BIOSYNTHESIS; CELL LINE; EPITHELIUM CELL; GENETIC TRANSCRIPTION; HUMAN; LUNG; METABOLISM; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE; EPITHELIAL CELLS; GLUTAMATE-CYSTEINE LIGASE; GLUTATHIONE; HUMANS; LUNG; RNA; TRANSCRIPTION, GENETIC; TRANSFORMING GROWTH FACTOR BETA;

EID: 0031280268     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.17.5.2833     Document Type: Article

References (50)

1. Crystal, R. G., J. D. Fulmer, W. C. Roberts, M. L. Moss, B. R. Line, and H. Y. Reynolds. 1976. Idiopathic pulmonary fibrosis: clinical, histologic, radiographic, physiologic, scintigraphic, cytologic, and biochemical aspects. Ann. Intern. Med. 85:769-788.
2. Cantin, A. M., S. L. North, R. C. Hubbard, and R. G. Crystal. 1987. Oxidant-mediated epithelial cell injury in idiopathic pulmonary fibrosis. J. Clin. Invest. 79:1665-1673.
3. Cantin, A. M., S. L. North, R. C. Hubbard, and R. G. Crystal. 1987. Normal alveolar epithelial lining fluid contains high levels of glutathione. J. Appl. Physiol. 63:152-157.
4. Cantin, A. M., R. C. Hubbard, and R. G. Crystal. 1989. Glutathione deficiency in the epithelial lining fluid of the lower respiratory tract in idiopathic pulmonary fibrosis. Am. Rev. Respir. Dis. 139:370-372.
5. Meister, A., and M. E. Anderson. 1983. Glutathione. Annu. Rev. Biochem. 52:711-760.
6. Martensson J., A. Jain, W. Frayer, and A. Meister. 1989. Glutathione metabolism in the lung: inhibition of its synthesis leads to lamellar body and mitochondrial defects. Proc. Natl. Acad. Sci. USA 86:5296-5300.
7. Shi, M. M., A. Kugelman, T. Iwamoto, L. Tan, and H. J. Forman. 1994. Ouinone-induced oxidative stress elevates glutathione and induces γ-glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269:26512-26517.
8. Limper, A. H., T. J. Broekelmann, T. V. Colby, G. Malizia, and J. A. McDonald. 1991. Analysis of local mRNA expression for extracellular matrix proteins and growth factors using in situ hybridization in fibroproliferative lung disorders. Chest 99(Suppl.):55S-56S.
9. Khalil, N., R. N. O'Connor, H. W. Unruh, P. W. Warren, K. C. Flanders, A. Kemp, O. H. Bereznay, and A. H. Greenberg. 1991. Increased production and immunohistochemical localization of transforming growth factor-β in idiopathic pulmonary fibrosis. Am. J. Respir. Cell Mol. Biol. 5:155-162.
10. 1 is present at sites of extracellular matrix gene expression in human pulmonary fibrosis. Proc. Natl. Acad. Sci. USA 88:6642-6646.
11. Border, W. A., and N. Noble. 1994. Transforming growth factor β in tissue fibrosis. N. Engl. J. Med. 331:1286-1292.
12. Border, W. A., and E. Ruoslahti. 1992. Transforming growth factor-β in disease: the dark side of tissue repair. J. Clin. Invest. 90:1-7.
13. 1 produces a "prooxidant" effect on bovine pulmonary artery endothelial cells in culture. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 262:L249-L254.
14. 1. Am. J. Respir. Cell Mol. Biol. 6:364-368.
15. 1 antiproliferative effects on endothelial cells by cysteine, cystine, and N-acetylctsteine. J. Clin. Invest. 90:1649-1656.
16. 1. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 265: L622-L626.
17. Rahman, I., X. Y. Li, K. Donaldson, D. J. Harrison, and W. MacNee. 1995. Glutathione homeostasis in alveolar epithelial cells in vitro and lung in vivo under oxidative stress. Am. J. Physiol. 269:L285-L292.
18. Kimball, R. E., K. Reddy, T. H. Peirce, L. W. Schwartz, M. G. Mustafa, and C. E. Cross. 1976. Oxygen toxicity: augmentation of antioxidant defense mechanisms in rat lung. Am. J. Physiol. 230:1425-1431.
19. Kugelman A., H. A. Choy, R. Liu, M. Ming Shi, E. Gozal, and H. J. Forman. 1994. γ-Glutamyl transpeptidase is increased by oxidative stress in rat alveolar L2 epithelial cells. Am. J. Respir. Cell Mol. Biol. 11:586-592.
20. Sun, J. D., J. A. Pickrell, J. R. Harkema, S. I. McLaughlin, F. F. Hahn, and R. F. Henderson. 1988. Effects of buthionine sulfoximine on the development of ozone-induced pulmonary fibrosis. Exp. Mol. Pathol. 49:254-266.
21. Lesur, O., A. M. Cantin, A. K. Tanswell, B. Melloni, J. F. Beaulieu, and R. Bégin. 1992. Silica exposure induces cytotoxicity and proliferation activity of type II pneumocytes. Exp. Lung Res. 18:173-190.
22. Cantin, A. M. 1994. Taurine modulation of hypochlorous acid-induced lung epithelial cell injury in vitro. J. Clin. Invest. 93:606-614.
23. Abei, H. 1984. Catalase in vitro. Methods Enzymol. 105:121-126.
24. Flohe, L., and W. A. Gümzler. 1976. Glutathione enzymatic oxidoreduction. Methods Enzymol. 77:17-34.
25. Horn, H. D. 1965. Glutathione reductase. In Methods of Enzymatic Analysis. H. V. Bergmeyer, editor. Academic Press, New York. 875-894.
26. Nardi, G., and M. Cipollaro. 1990. Assay of γ-glutamylcysteine synthetase and glutathione synthetase in erythrocytes by high-performance liquid chromatography with fluorometric detection. J. Chromatogr. 530:122-128.
27. Seelig, G. F., and A. Meister. 1984. γ-Glutamylcysteine synthetase from erythrocytes. Anal. Biochem. 141:510-514.
28. Sekura, R., and A. Meister. 1977. γ-Glutamylcysteine synthetase: further purification, half of the sites, reactivity, subunits, and specificity. J. Biol. Chem. 252:2599-2605.
29. Seelig, G. F., and A. Meister. 1985. Glutathione biosynthesis: γ-glutamylcysteine synthetase from rat kidney. Methods Enzymol. 113:379-390.
30. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
31. Pesce, M. A., and C. S. Strande. 1978. A new micromethod for the determination of protein in cerebrospinal fluid and urine. Clin. Chem. 19:1265-1267.
32. Chomczynski, P., and N. Sacchi. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
33. Gipp. J. J., C. Chang, and R. T. Mulcahy. 1992. Cloning and nucleotide sequence of a full-length cDNA for human liver γ-glutamylcysteine synthetase. Biochem. Biophys. Res. Commun. 185:29-35.
34. Warthoe, P., D. Bauer, M. Rohde, and M. Strauss. 1995. In PCR Primer: A Laboratory Manual. C. W. Dieffenbach and G. S. Dveksler, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 421-438.
35. Boudreau F., S. Blais, and C. Asselin. 1996. Regulation of CCAAT/enhancer binding protein isoforms by serum and glucocorticoids in the rat intestinal epithelial cell line IEC-6. Exp. Cell Res. 222:1-9.
36. Piechaczyk, M., J. M. Blanchard, L. Marty, C. Dani, F. Panatieres, S. El-Sabouty, P. Fort, and P. Jeanteur. 1984. Posttranscriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues. Nucleic Acids Res. 12:6951-6963.
37. 1 in rat hepatocytes. J. Biol. Chem. 269:15488-15492.
38. Meister, A. 1988. Glutathione metabolism and its selective modification. J. Biol. Chem. 263:17205-17208.
39. Huang, C. S., M. E. Anderson, and A. Meister. 1993. Amino acid sequence of the light subunit of rat kidney γ-glutamylcysteine synthetase. J. Biol. Chem. 268:20578-20583.
40. Yan, N., and A. Meister. 1990. Amino acid sequence of rat kidney γ-glutamylcysteine synthetase. J. Biol. Chem. 265:1588-1593.
41. Gipp, J. J., H. H. Bailey, and R. T. Mulcahy. 1995. Cloning and sequencing of the cDNA for the light subunit of human liver γ-glutamylcysteine synthetase and relative mRNA levels for heavy and light subunits in human normal tissues. Biochem. Biophys. Res. Commun. 206:584-589.
42. Huang, C. S., L. S. Chang, M. E. Anderson, and A. Meister. 1993. Catalytic and regulatory properties of rat kidney γ-glutamylcysteine synthetase. J. Biol. Chem. 268:19675-19680.
43. Mulcahy, R. T., and J. J. Gipp. 1995. Identification of a putative antioxidant response element in the 5′-flanking region of the human γ-glutamylcysteine synthetase heavy subunit gene. Biochem. Biophys. Res. Comm. 209: 227-233.
44. Kerr, L. D., D. B. Miller, and L. M. Matrisian. 1990. TGFβ inhibition of transin/stromelysin gene expression is mediated through a Fos binding sequence. Cell 61:267-278.
45. Pietenpol, J. A., K. Münger, P. W. Howley, R. W. Stein, and H. L. Moses. 1991. Factor-binding element in the human c-myc promoter involved in transcriptional regulation by transforminng growth factor β1 and by the retinoblastoma gene product. Proc. Natl. Acad. Sci. USA 88:10227-10231.
46. Poot, M., H. Teubert, P. S. Rabinovitch, and T. J. Kavanagh. 1995. De novo synthesis of glutathione is required for both entry into and progression through the cell cycle. J. Cell. Physiol. 163:555-560.
47. Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-β and contact inhibition to cell cycle arrest. Genes Dev. 8:9-22.
48. Anscher, M. S., W. P. Peters, H. Reisenbichler, W. P. Petros, and R. L. Jirtle. 1993. Transforming growth factor β as a predictor of liver and lung fibrosis after autologous bone marrow transplantation for advanced breast cancer. N. Engl. J. Med. 328:1592-1598.
49. Moreland, L. W., K. T. Goldsmith, W. J. Russell, K. R. Young, and R. I. Garver. 1992. Transforming growth factor β within fibrotic scleroderma lungs. Am. J. Med. 93:628-636.
50. Khalil, N., R. N. O'Connor, K. C. Flanders, W. Shing, and C. I. Whitman. 1994. Regulation of type II alveolar epithelial cell proliferation by TGF-β during bleomycin-induced lung injury in rats. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 267:L498-L507.