Purification and characterization of a Ca2+-dependent prothrombin activator, multactivase, from the venom of Echis multisquamatus

Journal of Biochemistry

Volumn 122, Issue 5, 1997, Pages 991-997

  Yamada, Daisuke ^a,b   Morita, Takashi ^a  

^a MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

^b JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

Author keywords

Calcium; Prothrombin activator; Prothrombin time; Venom metalloprotease; Warfarin

Indexed keywords

ANTICOAGULANT AGENT; CALCIUM ION; CHROMOGENIC SUBSTRATE; ECARIN; ENZYME ACTIVATOR; METALLOPROTEINASE; PROTHROMBIN; SNAKE VENOM; WARFARIN; CALCIUM; CARINACTIVASE; MULTACTIVASE; PEPTIDE; VIPER VENOM;

ARTICLE; DISULFIDE BOND; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBUNIT; HUMAN; NONHUMAN; ORAL DRUG ADMINISTRATION; PROTHROMBIN TIME; SNAKE; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; CALCIUM; ENZYME ACTIVATION; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTHROMBIN; SEQUENCE HOMOLOGY, AMINO ACID; VIPER VENOMS;

EID: 0031265396     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021862     Document Type: Article

References (38)

1. Mann, K.G., Nesheim, M.E., Church, W.R., Haley, P., and Krishnaswamy, S. (1990) Surface-dependent reactions of the vitamin K-dependent enzyme complexes. Blood 76, 1-16
2. Pirkle, H. and Markland, F.S., Jr. (1988) Hemostasis and Animal Venoms, Marcel Dekker, New York
3. Rosing, J. and Tans, G. (1992) Structural and functional properties of snake venom prothrombin activators. Toxicon 30, 1515-1527
4. Morita, T., Iwanaga, S., and Suzuki, T. (1976) The mechanism of activation of bovine prothrombin by an activator isolated from Echis carinatus venom and characterization of the new active intermediates. J. Biochem. 79, 1089-1108
5. Yamada, D., Sekiya, F., and Morita, T. (1996) Isolation and characterization of a carinactivase, a novel prothrombin activator in Echis carinatus venom with a unique catalytic mechanism. J. Biol. Chem. 271, 5200-5207
6. Yamada, D., Sekiya, F., and Morita, T. (1997) Prothrombin and factor X activator activities in the venoms of Viperidae snakes. Toxicon, in press
7. Hashimoto, N., Morita, T., and Iwanaga, S. (1985) A method for systematic purification from bovine plasma of six vitamin K-dependent coagulation factors: prothrombin, factor X, factor IX, protein S, protein C, and protein Z. J. Biochem. 97, 1347-1355
8. Morita, T., Nishibe, H., Iwanaga, S., and Suzuki, T. (1974) Studies on the activation of bovine prothrombin. J. Biochem. 76, 1031-1048
9. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
10. Hirano, H. (1989) Microsequence analysis of winged deanseed proteins electroblotted from two-dimensional gel. J. Protein Chem. 8, 115-130
11. Pirkle, H. and Marsh, N. (1991) Nomenclature of exogenous hemostatic factors. Thromb. Haemost. 66, 264
12. Nishida, S., Fujita, T., Kohno, N., Atoda, H., Morita, T., Takeya, H., Kido, I., Paine, M.J.I., Kawabata, S., and Iwanaga, S. (1995) cDNA cloning and deduced amino acid sequence of prothrombin activator (ecarin) from Kenyan Echis carinatus venom. Biochemistry 34, 1771-1778
13. Bjarnason, J.B. and Fox, J.W. (1995) Snake venom metalloproteases: Reprolysins. Methods Enzymol. 248, 345-368
14. Atoda, H., Hyuga, M., and Morita, T. (1991) The primary structure of coagulation factor IX/factor X-binding protein isolated from the venom of Trimeresurus flavoviridis. J. Biol. Chem. 266, 14903-14911
15. Sekiya, F., Atoda, H., and Morita, T. (1993) Isolation and characterization of an anti-coagulant protein homologous to botrocetin from the venom of Bothrops jararaca. Biochemistry 32, 6892-6897
16. Chen, Y-L. and Tsai, I-H. (1996) Functional and sequence characterization of coagulation factor IX/factor X-binding protein from the venom of Echis carinatus leucogaster. Biochemistry 36, 5264-5271
17. Peng, M., Lu, W., and Kirby, E.P. (1991) Alboaggregin-B: a new platelet agonist that binds to platelet membrane glycoprotein Ib. Biochemistry 30, 11529-11536
18. Fujimura, Y., Titani, K., Usami, Y., Suzuki, M., Oyama, R., Matsui, T., Fukui, H., Sugimoto, M., and Rugger, Z.M. (1991) Isolation and chemical characterization of two structurally and functionally distinct forms of botrocetin, the platelet coaggulutinin isolated from the venom of Bothrops jararaca. Biochemistry 30, 1957-1964
19. Zingali, R., Jandrot-Perrus, M., Guillin, M-C., and Bon, C. (1993) Bothrojaracin, a new thrombin inhibitor isolated from Bothrops jararaca venom: characterization and mechanism of thrombin inhibition. Biochemistry 32, 10794-10802
20. Tu, A.T. (1977) Venoms: Chemistry and Molecular Biology, pp. 329-360, John Wiley & Sons, New York
21. Takeya, H., Nishida, S., Miyata, T., Kawada, S., Saisaka, Y., Morita, T., and Iwanaga, S. (1992) Coagulation factor X activating enzyme from Russell's viper venom (RVV-X). J. Biol. Chem. 267, 14109-14117
22. Gowda, D.C., Jackson, C.M., Hensley, P., and Davidson, E.A. (1994) Factor X-activating glycoprotein of Russell's viper venom. J. Biol. Chem. 269, 10644-10650
23. Morita, T. Proteases Which Activate Factor X: Snake Venom Enzymes (Bailey, G.S., ed.). Alaken, Colorado, in press
24. Franssen, J.H.L., Janssen-Claessen, T., and Dieijen, G.V. (1983) Purification and properties of an activating enzyme of blood clotting factor X from the venom of Cerastes cerastes. Biochim. Biophys. Acta 747, 186-190
25. Hofmann, H. and Bon, C. (1987) Blood coagulation induced by the venom of Bothrops atrox. 2. Identification, purification, and properties of two factor X activators. Biochemistry 26, 780-787
26. Hite, L.A., Jia, L.-G., Bjarnason, J.B., and Fox, J.W. (1994) cDNA sequence for four snake venom metalloproteinases: structure, classification, and their relationship to mammalian reproductive proteins. Arch. Biochem. Biophys. 308, 182-191
27. Kini, R.M. (1995) Do we know the complete sequence of metalloproteinase and nonenzymatic platelet aggregation inhibitor (disintegrin) precursor proteins? Toxicon 33, 1151-1160
28. Jia, L.-G., Shimokawa, K., Bjarnason, J.B., and Fox, J.W. (1996) Snake venom metalloproteinases: structure, function and relationship to the ADAMs family of proteins. Toxicon 34, 1269-1276
29. Kini, R.M. (1996) Are C-type lectin-related proteins derived by proteolysis of metalloproteinase/disintegrin precursor proteins? Toxicon 34, 1287-1294
30. Matsuzaki, R., Yoshihara, E., Yamada, M., Shima, K., Atoda, H., and Morita, T. (1996) cDNA cloning of IX/X-bp, a heterogeneous two-chain anticoagulant protein from snake venom. Biochem. Biophys. Res. Commun. 220, 382-387
31. Mizuno, H., Fujimoto, Z., Koizumi, M., Kano, H., Atoda, H., and Morita, T. (1997) Structure of coagulant factor IX/factor X-binding protein, a heterodimer of C-type lectin domains. Nature Struct. Biol. 4, 438-441
32. Solovjov, D.A., Platonova, T.N., and Ugarova, T.P. (1996) Purification and characterization of ecamulin - a new prothrombin activator from Echis multisquamatus snake venom. Biochemistry (Moscow) 61, 785-793. Translated from Biokhimiya 61, 1094-1105
33. Solovjov, D.A., Platonova, T.N., and Ugarova, T.P. (1996) Purification and characterization of ecamulin - a new prothrombin activator from Echis multisquamatus snake venom. Biochemistry (Moscow) 61, 785-793. Translated from Biokhimiya 61, 1094-1105
34. Furie, B., Liebman, H.A., Blanchard, R.A., Coleman, M.S., Kruger, S.F., and Furie, B.C. (1984) Comparison of the native prothrombin antigen and the prothrombin time for monitoring oral anticoagulant therapy. Blood 64, 445-451
35. Furie, B., Diuguid, C.F., Jacobs, M., Diuguid, D.L., and Furie, B.C. (1990) Randomized prospective trial comparing the native prothrombin antigen with the prothrombin time for monitoring oral anticoagulant therapy. Blood 75, 344-349
36. Kornberg, A., Francis, C.W., Pellegrini, V.D. Jr., Gabriel, K.R., and Marder, V.J. (1993) Comparison of native prothrombin antigen with the prothrombin time for monitoring oral anti-coagulant prophylaxis. Circulation 88, 454-460
37. Paul, B., Oxley, A., Brigham, K., Cox, T., and Hamilton, P.J. (1987) Factor II, VII, IX and X concentrations in patients receiving long term warfarin. J. Clin. Pathol. 40, 94-98
38. Xi, M., Béguin, S., and Hemker, H.C. (1989) The relative importance of the factors II, VII, IX and X for the prothrombinase activity in plasma of orally anticoagulated patients. Thromb. Haemost. 62, 788-791