A Streamlined Mutation Detection System: Multicolor Post-PCR Fluorescence Labeling and Single-Strand Conformational Polymorphism Analysis by Capillary Electrophoresis

Genome Research

Volumn 7, Issue 11, 1997, Pages 1094-1103

  Inazuka, Masakazu ^a   Wenz, Hans Michael ^b   Sakabe, Munechika ^c   Tahira, Tomoko ^a   Hayashi, Kenshi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b APPLIED BIOSYSTEMS   (United States)

^c PE Applied Biosystems Japan Inc   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING);

ARTICLE; AUTOMATION; CALIBRATION; CAPILLARY ELECTROPHORESIS; CHROMOSOME MAP; EXON; GENETICS; GENOME; HUMAN; METHODOLOGY; MUTATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SINGLE STRAND CONFORMATION POLYMORPHISM;

AUTOMATION; CALIBRATION; CHROMOSOME MAPPING; DNA GYRASE; DNA MUTATIONAL ANALYSIS; DNA TOPOISOMERASES, TYPE II; ELECTROPHORESIS, CAPILLARY; EXONS; GENOME, HUMAN; HUMANS; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SENSITIVITY AND SPECIFICITY;

EID: 0031263204     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.7.11.1094     Document Type: Article

References (17)

1. Chee, M., R. Yang, E. Hubbell, A. Berno, X.C. Huang, D. Stern, J. Winkler, D. Lockhart, M.S. Morris, and S.P.A. Fodor. 1996. Accessing genetic information with high density DNA arrays. Science 274: 610-614.
2. Ellison, J., M. Dean, and D. Goldman. 1993. Kfficacy of fluorescence-based PCR-SSCP for detection of point mutation. BioTechniques 15: 684-691.
3. Felmlee, T.A., R.P. Oda, D.A. Persing, and J.P. Landers. 1995. Capillary electrophoresis of DNA potential utility for clinical diagnoses. J. Chromatogr. B. 717: 127-137.
4. Hayashi, K. and D.W. Yandell. 1993. How sensitive is PCR-SSCP? Hum. Mutat. 2: 338-346.
5. Hebenbrock, K., P.M. Williams, and R.L. Karger. 1995. Single strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection. Electrophoresis 16: 1429-1436.
6. Inazuka, M., T. Tahira, and K. Hayashi. 1996. One-tube post-PCR fluorescent labeling of DNA fragments. Genome Res. 6: 551-557.
7. Iwahana, H., K. Yoshimoto, N. Mizusawa, E. Kudo, and M. Itakura. 1994. Multiple fluorescence-based PCR-SSCP analysis. BioTechniques 16: 296-305.
8. Iwahana, H., K. Adzuma, Y. Takahashi, R. Katashima, K. Yoshimoto, and M. Itakura. 1995. Multiple fluorescence-based PCR-SSCP analysis with postlabeling. PCR Methods Applic. 4: 275-282.
9. Kukita, Y., T. Tahira, S.S. Sommer, and K. Hayashi. 1997. SSCP analysis of long DNA fragments In low pH gel. (in press).
10. Kuypers, A.W., P. Williams, M. vander Schans, P. Linssen, H. Wessels, C. de Bruijn, F. Everaerts, and D. Mensink. 1993. Detection of point mutations in DNA using capillary electrophoresis in a polymer network. J. Chromatogr. 621: 149-156.
11. Landegren, U. 1996. Mutation detection now and later. In Laboratory protocols for mutation detection (ed. U. Landegren). Oxford University Press, Oxford, UK.
12. Makino, R., H. Yazyu, Y. Kishimoto, T. Sekiya, and K. Hayashi. 1992. F-SSCP: Fluorescence-based polymerase chain react ion-single-strand conformation polymorphism (PCR-SSCP) analysis. PCR Methods Applic. 2: 10-13.
13. Munakata, N., F. Morohashi, M. Saitou, N. Yamazaki, and K. Hayashi. 1994. Molecular characterization of thirteen gyrA mutations conferring nalidixic acid resistance in Bacillus subtilis. Mol. & Gen. Genet. 244: 97-103.
14. Orita, M., Y. Suzuki, T. Sekiya, and K. Hayashi. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5: 874-879.
15. Oto, M., T. Suehiro, and Y. Yuasa. 1995. Identification of mutated p53 in cancer by non-gel-sieving capillary electrophoretic SSCP analysis. Clin. Chem. 41: 1787-1788.
16. Rodbard, D. and A. Chrambach. 1970. Unified theory for gel electrophoresis and gel filtration. Proc. Natl. Acad. Sci. 65: 970-977.
17. Yoshitake, S., B.G. Schach, D.C. Foster, E.W. Davie, and K. Kurachi. 1985. Nucleotide sequence of the gene for human factor IX (antihemophilic factor B). Biochemistry 24: 3736-3750.