Spinal Cord Transection - No Loss of Distal Ventral Horn Neurons: Modern Stereological Techniques Reveal No Transneuronal Changes in the Ventral Horns of the Mouse Lumbar Spinal Cord after Thoracic Cord Transection

Experimental Neurology

Volumn 148, Issue 1, 1997, Pages 179-186

  Bjugn, Roger ^a,b   Nyengaard, Jens R ^b   Rosland, Jan Henrik ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ANTERIOR HORN CELL; ARTICLE; CELL COUNT; CELL SIZE; CORDOTOMY; EFFERENT NERVE; ENDOTHELIUM; GLIA; MALE; MOUSE; NERVE CELL; NERVE DEGENERATION; NEUROPIL; PATHOLOGY; SPINAL CORD; SPINAL CORD INJURY;

ANIMALS; ANTERIOR HORN CELLS; CELL COUNT; CELL SIZE; CORDOTOMY; EFFERENT PATHWAYS; ENDOTHELIUM; MALE; MICE; NERVE DEGENERATION; NEUROGLIA; NEURONS; NEUROPIL; SPINAL CORD; SPINAL CORD INJURIES;

EID: 0031262520     PISSN: 00144886     EISSN: None     Source Type: Journal    
DOI: 10.1006/exnr.1997.6610     Document Type: Article

References (33)

1. Bjugn, R. 1993. The use of the optical disector to estimate the number of neurons, glial and endothelial cells in the spinal cord of the mouse - with a comparative note on the rat spinal cord. Brain Res. 627: 25-33.
2. Bjugn, R., and H. J. G. Gundersen. 1993. Estimate of the total number of neurons and glial and endothelial cells in the rat spinal cord by means of the optical disector. J. Comp. Neurol. 328: 406-411.
3. Cheng, H., Y. Cao, and L. Olson. 1996. Spinal cord repair in adult paraplegic rats: Partial restoration of hind limb function. Science 273: 510-513.
4. Chimelli, L., and F. Scaravilli. 1987. The development of the gracile nucleus in the rat: The time of ingrowth of ascending primary sensory fibres and effect of early deafferentation. Neuroscience 22: 661-670.
5. Coggeshall, R. E. 1992. A consideration of neural counting methods. Trends Neurosci. 15: 9-13.
6. Coggeshall, R. E., and H. A. Lekan. 1996. Methods for determin-ing numbers of cells and synapses: A case for more uniform standards of review. J. Comp. Neurol. 364: 6-15.
7. Cook, W. H., J. H. Walker, and M. L. Barr. 1951. A cytological study of transneuronal atropohy in the cat and rabbit. J. Comp. Neurol. 94: 267-291.
8. Cowan, W. M. 1970. Anterograde and retrograde transneuronal degeneration in the central and peripheral nervous system. In Contemporary Research Methods in Neuroanatomy (W. J. H. Nauta and S. O. E. Ebbesson, Eds.), pp. 217-251. Springer-Verlag, Berlin.
9. Das, G. D., K. G. Das, J. Brasko, M. Riedl, P. Rai, and V. Rajeswari. 1989. Spinal traumas: Some postoperative complications in experimental animals. Brain Res. Bull. 22: 33-37.
10. Duchen, L. W. 1992. General pathology of neurons and neuroglia. In Greenfield's Neuropathology (J. H. Adams and L. W. Duchen, Eds.), pp. 1-68. Oxford Univ. Press, New York.
11. Eidelberg, E., L. H. Nguyen, R. Polich, and J. G. Walden. 1989. Transsynaptic degeneration of motoneurones caudal to spinal cord lesions. Brain Res. Bull. 22: 39-45.
12. Gundersen, H. J. G., P. Bagger, T. F. Bendtsen, S. M. Evans, L. Korbo, N. Marcussen, A. Møller, K. Nielsen, J. R. Nyengaard, B. Pakkenberg, F. B. Sørensen, A. Vesterby, and M. J. West. 1988. The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 96: 857-881.
13. Gundersen, H. J. G., T. F. Bendtsen, L. Korbo, N. Marcussen, A. Møller, K. Nielsen, J. R. Nyengaard, B. Pakkenberg, F. B. Sørensen, A. Vesterby, and M. J. West. 1988. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96: 379-394.
14. Gundersen, H. J. G., and E. B. Jensen. 1987. The efficiency of systematic sampling in stereology and its prediction. J. Microsc. 147:229-263.
15. Hama, A. T., G. D. Pappas, and J. Sagen. 1996. Adrenal medullary implants reduce transsynaptic degeneration in the spinal cord of rats following chronic constriction nerve injury. Exp. Neurol. 137: 81-93.
16. Jensen, E. B. V., and H. J. G. Gundersen. 1993. The rotator. J. Microsc. 170: 35-44.
17. Kaelan, C., P. F. Jacobsen, and B. A. Kakulas. 1988. An investigation of possible transynaptic neuronal degeneration in human spinal cord injury. J. Neurol. Sci. 86: 231-237.
18. Knyihar Csillik, E., P. Rakic, and B. Csillik. 1989. Transneuronal degeneration in the Rolando substance of the primate spinal cord evoked by axotomy-induced transganglionic degenerative atrophy of central primary sensory terminals. Cell Tissue Res. 258: 515-525.
19. Kuypers, H. G. J. M. 1981. Anatomy of the descending pathways. In Handbook of Physiology. 1. The Nervous System (V. B. Brooks, Ed.), Volume II, Motor Control, Part 1, pp. 599-666. Am. Physiol. Soc. Baltimore, MD.
20. Larsen, J. O., T. Tandrup, and H. Braendgaard. 1994. The volume of Purkinje cells decreases in the cerebellum of acrylamide-intoxicated rats, but no cells are lost. Acta Neuropathol. (Berlin) 88:307-312.
21. Marsala, J., I. Sulla, P. Jalc, and J. Orendacova. 1995. Multiple protracted cauda equina constrictions cause deep derangement in the lumbosacral spinal cord circuitry in the dog. Neurosci. Lett. 193: 97-100.
22. Martin, T. P., S. Bodine Fowler, and V. R. Edgerton. 1988. Coordination of electromechanical and metabolic properties of cat soleus motor units. Am. J. Physiol. 255: C684-93.
23. Mayhew, T. M., and H. J. G. Gundersen. 1996. "If you assume, you can make an ass out of u and me": A decade of the disector for stereological counting of particles in 3D space. J. Anat. 188: 1-15.
24. McBride, R. L., and E. R. Feringa. 1992. Ventral horn motoneurons 10, 20 and 52 weeks after T-9 spinal cord transection. Brain Res. Bull. 28: 57-60.
25. Noble, L. J., and J. R. Wrathall. 1987. The blood-spinal cord barrier after injury: Pattern of vascular events proximal and distal to a transection in the rat. Brain Res. 424: 177-188.
26. Okado, N., and R. W. Oppenheim. 1984. Cell death of motoneurons in the chick embryo spinal cord. IX. The loss of motoneurons following removal of afferent inputs. J. Neurosci. 4: 1639-1652.
27. Oorschot, D. E. 1994. Are you using neuronal densities, synaptic densities or neurochemical densities as your definitive data? There is a better way to go. Prog. Neurobiol. 44: 233-247.
28. Pierotti, D. J., R. R. Roy, S. C. Bodine Fowler, J. A. Hodgson, and V R. Edgerton. 1991. Mechanical and morphological properties of chronically inactive cat tibialis anterior motor units. J. Physiol. (London) 444: 175-192.
29. Rosland, J. H., and K. Hole. 1990. Benzodiazepine-induced antagonism of opioid antinociception may be abolished by spinalization or blocade of the benzodiazepine receptor. Pharmacol. Biochem. Behav. 37: 505-509.
30. Stelzner, D. J., W. B. Ersiiler, and E. D. Weber. 1975. Effects of spinal transection in neonatal and weanling rats: Survival of function. Exp. Neurol. 46: 156-177.
31. Tracey, D. J. 1995. Ascending and descending pathways in the spinal cord. In The Rat Nervous System (G. Paxinos, Ed.), pp. 67-80. Academic Press, San Diego.
32. Yin, Q.-W., J. Johnson, D. Prevette, and R. W. Oppenheim. 1994. Cell death of spinal motoneurons in the chick embryo following deafferentation: Rescue effects of tissue extracts, soluble proteins, and neurotrophic agents. J. Neurosci. 14: 7629-7640.
33. Young, I. J. 1966. Morphological and histochemical studies of partially and totally deafferented spinal cord segments. Exp. Neurol. 14: 238-248.