Optimization, assessment, and proposed use of a direct nested reverse transcription-polymerase chain reaction protocol for the detection of hepatitis C virus

Journal of Human Virology

Volumn 1, Issue 1, 1997, Pages 58-65

  Abdel Hamid, Mohamed ^a,b   Edelman, Daniel C ^a   Highsmith, W Edward ^a   Constantine, Niel T ^a,c  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b MINIA UNIVERSITY   (Egypt)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

Diagnostics; Hepatitis; Hepatitis C virus; Molecular biology; Reverse transcription polymerase chain reaction (RT PCR) assay

Indexed keywords

ALGORITHM; ARTICLE; COST BENEFIT ANALYSIS; HEPATITIS C VIRUS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ALGORITHMS; COST-BENEFIT ANALYSIS; HEPACIVIRUS; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0031261060     PISSN: 10909508     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Alter HJ, Tegtmeier GE, Jett BW, et al. The use of a recombinant immunoblot assay in the interpretation of anti-hepatitis C virus reactivity among prospectively followed patients, implicated donors, and random donors. Transfusion 1991;31:771-776.
2. Callahan JD, Constantine NT, Kataaha P, Zhang X, Hyams KC, Bansal J. Second generation hepatitis C virus assays: performance when testing African sera. J Med Virol 1993; 41:35-38.
3. Bansal J, Constantine NT, Zhang X, Callahan JD, Marsiglia V, Hyams KC. Evaluation of five hepatitis C virus screening tests and two supplemental assays: performance when testing sera from sexually transmitted diseases clinic attendees in the USA. Clin Diagn Virol 1993;1:113-121.
4. Chokephaibulkit K, Painter PC, Patamasucon P. Overview of hepatitis C. Lab Med 1992;23:798-803.
5. Zaaijer HL, Vrielink H, Van Exel-Oehlers PJ, Cuypers HTM, Lelie PN. Confirmation of hepatitis C infection: a comparison of five immunoblot assays. Transfusion 1994; 7:603-607.
6. Dhaliwal SK, Prescott LE, Dow BC, et al. Influence of viremia and genotype upon serological reactivity in screening assays for antibody to hepatitis C virus. J Med Virol 1996;48:184-190.
7. Miskovsky EP, Carrella AV, Gutekunst K, Sun CA, Quinn TC, Thomas DL. Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus. J Clin Microbiol 1996;34:1975-1979.
8. Alter MJ. Review of serologic testing for hepatitis C virus infection and risk of post-transfusion hepatitis. Clin Arch Pathol Lab Med 1994;118:342-345.
9. Farci P, Alter HJ, Govindarajan S, et al. Lack of protective immunity against reinfection with hepatitis C virus. Science 1992:258:135-40.
10. Okamoto H, Sugiyama Y, Okada S, et al. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol 1992;73:673-679.
11. French Study Group for the Standardization of Hepatitis C Virus PCR. Improvement of hepatitis C virus RNA polymerase chain reaction through a multicentre quality control study. French study group for the standardization of hepatitis C virus PCR. J Virol Methods 1994;49:79-88.
12. Hu KQ, Yu CH, Vierling JM. One-step RNA polymerase chain reaction for detection of hepatitis C virus RNA. Hepatology 1993;18:270-274.
13. Young KKY, Resnick RM, Myers TW. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J Clin Microbiol 1993; 31:882-886.
14. Okamoto M, Baba M, Kodama E, et al. Detection of hepatitis C virus genome in human serum by multi-targeted polymerase chain reaction. J Med Virol 1993;41:6-10.
15. Li JS, Tong SP, Vitvitski L, Trepo C. Single-step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus. J Med Virol 1995;45:151-155.
16. Navas S, Carreno V. Semiquantification by Amplicor assay of hepatitis C virus genome during therapy. Hepatology 1995;22:115-117.
17. Zaaijer HL, Cuypers HTM, Reesink HW, Winkel IN, Gerken G, Lelie PN. Reliability of polymerase chain reaction for detection of hepatitis C virus. Lancet 1993;341: 722-24.
18. Kedzierski W, Porter JC. A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures. Biotechniques 1991;10:210-214.
19. Inui A, Fujisawa T, Komatsu H. Rapid detection of hepatitis C virus by direct reverse transcription-polymerase chain reaction. Int Hepatol Commun 1994;3:126-130.
20. Tjhie JHT, van Kuppeveld FJM, Roosendaal R, et al. Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections. J Clin Microbiol 1994;32:11-16.
21. Klebe RJ, Grant GM, Grant AM, Garcia MA, Giambernardi TA, Taylor GP. RT-PCR without RNA isolation. Biotechniques 1996;21:1094-1100.
22. Marin MG, Bresciani S, Puoti M, et al. Clinical significance of serum hepatitis C virus (HCV) RNA as marker of HCV infection. J Clin Microbiol 1994;32:3008-3012.
23. Pistello M, Maggi F, Vatteroni L, et al. Prevalence of hepatitis C virus genotypes in Italy. J Clin Microbiol 1994;32: 232-234.
24. Constantine NT, Abdel-Hamid M, Oldach D. Rapid genotyping of hepatitis C virus. N Engl J Med 1995;333:800.
25. Ali N, Jameel S. Direct detection of hepatitis C virus RNA in serum by reverse transcription PCR. Biotechniques 1993;15:40.
26. Shieh YSC, Wait D, Tai L, Sobsey MD. Methods to remove inhibitors in sewage and other fecal wastes for enterovirus detection by the polymerase chain reaction. J Virol Methods 1995;54:51-66.
27. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
28. Okamoto H, Okada S, Sugiyama Y, et al. The 5′-terminal sequence of the hepatitis C virus genome. Jpn J Exp Med 1990;60:167-177.
29. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-238.
30. Innis MA, Gelfand DH, Sninsky JJ. PCR strategies. San Diego, CA: Academic Press, 1995:98-108.