Purification and characterization of recombinant tomato fruit (Lycopersicon esculentum Mill.) Fructokinase expressed in Escherichia coli

Protein Expression and Purification

Volumn 11, Issue 1, 1997, Pages 41-46

  Martinez Barajas, Eleazar ^a,c   Krohn, Bradley M ^b   Stark, David M ^b   Randall, Douglas D ^a  

^a University of Missouri   (United States)

^b MONSANTO COMPANY   (United States)

^c UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LYCOPERSICON ESCULENTUM;

EID: 0031260039     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0762     Document Type: Article

References (21)

1. 1. Copeland, L., Harrison, D. D., and Turner, J. F. (1978) Fructokinase (Fraction III) of pea seeds. Plant Physiol. 62, 291-294.
2. 2. Doehlert, D. C. (1989) Separation and characterization of four hexose kinases from developing maize kernels. Plant Physiol. 89, 1042-1048.
3. 3. Renz, A., Merlo, L., and Stitt, M. (1993) Partial purification from potato tubers of three fructokinases and three hexosekinases which show differing organ and developmental specificity. Planta 190, 156-165.
4. 4. Chaubron, F., Harris, N., Ross, H. A., and Davies, H. V. (1995) Partial purification and characterization of fructokinase from developing taproots of sugar beet (Beta vulgaris). Plant Sci. 110, 181-186.
5. 5. Martinez-Barajas, E., and Randall, D. D. (1996) Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum Mill.) fruits. Planta 199, 451-458.
6. 6. Geigenberger, P., and Stitt, M. (1993) Sucrose synthase catalyzes a readily reversible reaction in vivo in developing potato tubers and other plant tissues. Planta 189, 329-339.
7. 7. Viola, R. (1996) Hexose metabolism in discs excised from developing potato (Solanum tuberosum L.) tubers. II. Estimations of fluxes in vivo and evidence that fructokinase catalyzes a near rate-limiting reaction. Planta 198, 186-196.
8. 8. Copeland, L., Stone, S. R., and Turner, J. F. (1984) Kinetic studies of fructokinase I of pea seeds. Arch. Biochem. Biophys. 233, 748-760.
9. 9. Renz, A., and Stitt, M. (1993) Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers. Planta 190, 166-175.
10. 10. Gardner, A., Davies, H. V., and Burch, L. R. (1992) Purification and properties of fructokinase from developing tubers of potato (Solanum tuberosum L.). Plant Physiol. 100, 178-183.
11. 11. Doehlert, D. C. (1990) Fructokinase from developing maize kernels differ in their specificity for nucleoside triphosphates. Plant Physiol. 93, 353-355.
12. 12. Smith, S. B., Taylor, M. A., Burch, L. R., and Davies, H. V. (1993) Primary structure and characterization of a cDNA clone of fructokinase from potato (Solanum tuberosum L. cv. Record). Plant Physiol. 102, 1043.
13. 13. Martinez-Barajas, E., Luethy, M. H., and Randall, D. D. (1997) Molecular cloning and analysis of fructokinase expression in tomato (Lycopersicon esculentum Mill.). Plant Sci. 125, 13-20.
14. 14. Kanayama, Y., Day, N., Granot, D., Petreikov, M., Schaffer, A., and Bennet, A. B. (1997) Divergent fructokinase genes are differentially expressed in tomato. Plant Physiol. 113, 1379-1384.
15. 15. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) "Molecular Cloning: A Laboratory Manual," Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. 16. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
17. 17. Olins, P. O., and Rangwala, S. H. (1990) Vector for enhanced translation of foreign genes in Escherichia coli. Methods Enzymol. 185, 115-119.
18. 18. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
19. 19. Barent, R. L., and Elthon, T. E. (1992) Two-dimensional gels: An easy method for large quantities of proteins. Plant Mol. Biol. Rep. 10, 330-344.
20. 20. Turner, J. F., Chensee, Q. J., and Harrison, D. D. (1977) Glucokinase of pea seeds. Biochim. Biophys. Acta 480, 367-375.
21. 21. Spangfort, M. D., Ipsen, H., Sparholt, S. H., Aasmul-Olsen, S., Larsen, M. R., Mørtz, E., Roepstorff, P., and Larsen, J. N. (1996) Characterization of purified recombinant Bet v 1 with authentic N-terminus, cloned in fusion with maltose-binding protein. Protein Expression Purif. 8, 365-373.