Thrombin-mediated Focal Adhesion Plaque Reorganization in Endothelium: Role of Protein Phosphorylation

American Journal of Respiratory Cell and Molecular Biology

Volumn 17, Issue 4, 1997, Pages 443-455

  Schaphorst, Kane L ^b   Pavalko, Frederick M ^b   Patterson, Carolyn E ^b   Garcia, Joe G N ^a  

^a INDIANA UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION MOLECULE; FOCAL ADHESION KINASE; FOCAL ADHESION KINASE 1; PROTEIN TYROSINE KINASE; TALIN; THROMBIN; VINCULIN;

ANIMAL; ARTICLE; CATTLE; CELL CULTURE; CELL JUNCTION; CYTOLOGY; DRUG EFFECT; METABOLISM; PHOSPHORYLATION; PULMONARY ARTERY; ULTRASTRUCTURE; VASCULAR ENDOTHELIUM;

ANIMALS; CATTLE; CELL ADHESION MOLECULES; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; FOCAL ADHESION KINASE 1; FOCAL ADHESION PROTEIN-TYROSINE KINASES; GAP JUNCTIONS; PHOSPHORYLATION; PROTEIN-TYROSINE KINASES; PULMONARY ARTERY; TALIN; THROMBIN; VINCULIN;

EID: 0031255581     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.17.4.2502     Document Type: Article

References (57)

1. Majno, G., and G. E. Palade. 1991. Studies of inflammation. The effect of histamine and serotonin on vascular permeability: an electron microscope study. J. Biophys. Biochem. Cytol. 11:571-605.
2. Lum, H., and A. B. Malik. 1994. Regulation of vascular endothelial barrier function. Am. J. Physiol. 267:L223-L241.
3. Garcia, J. G. N., and K. L. Schaphorst. 1995. Regulation of endothelial cell gap formation and paracellular permeability. J. Invest. Med. 43:117-126.
4. Wysolmerski, R. B., and D. Lagunoff. 1991. Regulation of permeabilized endothelial cell retraction by myosin phosphorylation. Am. J. Physiol. 261: C32-C40.
5. Moy, A. B., S. S. Shasby, B. D. Scott, and D. M. Shasby. 1993. The effect of histamine and cyclic adenosine monophosphate on myosine light chain phosphorylation in human umbilical vein endothelial cells. J. Clin. Invest. 92:1198-1206.
6. Sheldon, R., A. Moy, K. Lindsley, S. Shasby, and D. M. Shasby. 1993. Role of myosin light-chain phosphorylation in endothelial cell retraction. Am. J. Physiol. 265:L606-L612.
7. Garcia, J. G. N., H. W. Davis, and C. E. Patterson. 1945. Regulation of endothelial cell gap formation and barrier dysfunction: role of myosin light chain phosphorylation. J. Cell Physiol. 163:510-522.
8. Kolodney, M. S., and R. B. Wysolmerski. 1992. Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study. J. Cell Biol. 117:73-82.
9. Garcia, J. G., V. Lazar, L. I. Gilbert-McClain, P. J. Gallagher, and A. D. Verin. 1997. Myosin light chain kinase in endothelium: molecular cloning and regulation. Am. J. Respir. Cell Mol. Biol. (In press)
10. 2+ mobilization, barrier dysfunction, prostaglandin synthesis, and platelet-derived growth factor mRNA expression in cultured endothelium. J. Cell Physiol. 156:541-549.
11. Stasek, J. E., Jr., C. E. Patterson, and J. G. Garcia. 1992. Protein kinase C phosphorylates caldesmon77 and vimentin and enhances albumin permeability across cultured bovine pulmonary artery endothelial cell monolayers. J. Cell Physiol. 153:62-75.
12. Patterson, C. E., J. E. Stasek, K. L. Schaphorst, H. W. Davis, and J. G. N. Garcia. 1995. Mechanisms of pertussis toxin-induced barrier dysfunction in bovine pulmonary artery endothelial monolayers. Am. J. Physiol. 268: L926-L934.
13. Patterson, C. E., J. E. Stasek, M. A. Goddard, A. Farber, and J. G. Garcia. 1994. Signal transduction events in interleukin-1-induced endothelial cell barrier dysfunction. Am. Rev. Respir. Dis. 149:A823. (Abstr.)
14. Garcia, J. G., K. L. Schaphorst, C. M. Hart, K. Callahan, and C. E. Patterson. 1997. Mechanisms of ionomycin-induced endothelial permeability. Am. J. Physiol. (In press)
15. Pasquale, E. B., P. A. Maher, and S. J. Singer. 1986. Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus. Proc. Natl. Acad. Sci. USA 83:5507-5511.
16. Harrington, M. A., R. Daub, A. Song, J. Stasek, and J. G. Garcia. 1992. Interleukin 1 alpha mediated inhibition of myogenic terminal differentiation: increased sensitivity of Ha-ras transformed cultures. Cell Growth Differ. 3:241-248.
17. Burridge, K., G. Nuckolls, C. Otey, F. Pavalko, K. Simon, and C. Turner. 1990. Actin-membrane interaction in focal adhesions. Cell Differ. Dev. 32:337-342.
18. Parsons, J. T., M. D. Schaller, J. Hildebrand, T. H. Leu, A. Richardson, and C. Otey. 1994. Focal adhesion kinase: structure and signalling. J. Cell Sci. Suppl. 18:109-113.
19. Defilippi, P., C. Bozzo, G. Volpe, G. Romano, M. Venturino, L. Silengo, and G. Tarone. 1994. Integrin-mediated signal transduction in human endothelial cells: analysis of tyrosine phosphorylation events. Cell Adhesion Commun. 2:75-86.
20. Pavalko, F. M., and K. Burridge. 1991. Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of alpha-actinin. J. Cell Biol. 114:481-491.
21. Shasby, D. M., S. S. Shashy, J. M. Sullivan, and M. J. Peach. 1982. Role of endothelial cell cytoskeleton in control of endothelial permeability. Circ. Res. 51:657-661.
22. Patterson, C. E., R. A. Rhoades, and J. O. Garcia. 1992. Evans Blue dye as a marker of albumin clearance in cultured endothelial monolayer and isolated lung. J. Appl. Physiol. 72:865-873.
23. Tiruppathi, C., A. B. Malik, P. J. Del Vecchio, C. R. Keese, and I. Giaever. 1992. Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function. Proc. Natl. Acad. Sci. USA 89:7919-7923.
24. Hathaway, D. R., and R. S. Adelstein. 1979. Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity. Proc. Natl. Acad. Sci. USA 76:1653-1657.
25. Garcia, J. G. N., A. Siflinger-Birnboim, R. Bizios, P. J. Del Vecchio, J. W. Fenton, II, and A. B. Malik. 1986. Thrombin-induced increase in albumin permeability across the endothelium. J. Cell Physiol. 128:96-104.
26. Jaken, S., K. Leach, and T. Klauck. 1989. Association of type 3 protein kinase C with focal contacts in rat embryo fibroblasls. J. Cell Biol. 109:697-704.
27. Clark, E. A., S. J. Shattil, M. H. Ginsberg, J. Bolen, and J. S. Brugge. 1994. Regulation of the protein tyrosine kinase pp72syk by platelet agonists and the integrin alpha IIb beta 3. J. Biol. Chem. 269:28859-28864.
28. Lipfert, L., B. Haimovich, M. D. Schaller, B. S. Cobb, J. T. Parsons, and J. S. Brugge. 1992. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets. J. Cell Biol. 119:905-912.
29. Schaller, M. D., and J. T. Parsons. 1994. Focal adhesion kinase and associated proteins. Curr. Opin. Cell Biol. 6:705-710.
30. 2+ and inositol polyphosphates. Am. J. Physiol. 263:L219-L225.
31. Stasek, J. E., Jr., and J. G. Garcia. 1992. The role of protein kinase C in alpha-thrombin-mediated endothelial cell activation. Semin. Thromb. Hemostasis 18:117-125.
32. Jacobson, B. C., J. S. Pober, J. W. Fenton, II, and B. M. Ewenstein. 1992. Thrombin and histamine rapidly stimulate the phosphorylation of the myristoylated alanine-rich C-kinase substrate in human umbilical vein endothelial cells: evidence for distinct patterns of protein kinase activation. J. Cell Physiol. 152:166-176.
33. Pavalko, F. M., G. Screider, K. Burridge, and S. S. Lim. 1995. Immunodetection of alpha-actinin in focal adhesions is limited by antibody accessibility. Exp. Cell Res. 217:534-540.
34. Otey, C. A., F. M. Pavalko, and K. Burridge. 1990. An interaction between alpha-aclinin and the beta 1 integrin subunit in vitro. J. Cell Biol. 111:721-729.
35. Lampugnani, M. G., M. Resnati, E. Dejana, and P. C. Marchisio. 1991. The role of integrins in the maintenance of endothelial monolayer integrity. J. Cell Biol. 112:479-490.
36. Romer, L. H., N. McLean, C. E. Turner, and K. Burridge. 1994. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. Mol. Biol. Cell 5:349-361.
37. Beckerle, M. C., K. Burridge, G. N. DeMartino, and D. E. Croall. 1987. Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion. Cell 51:569-577.
38. Cobb, B. S., M. D. Schaller, T. H. Leu, and J. T. Parsons. 1994. Stable association of pp60src and pp59fyn with the focal adhesion-associated protein tyrosine kinase, pp125FAK. Mol. Cell. Biol. 14:147-155.
39. Schaller, M. D., C. A. Borgman, B. S. Cobb, R. R. Vines, A. B. Reynolds, and J. T. Parsons. 1992. pp125FAK: a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA 89:5192-5196.
40. Wong, M. K., and A. I. Gotlieb. 1990. Endothelial monolayer integrity. Perturbation of F-actin filaments and the dense peripheral band-vinculin network. Arteriosclerosis 10:76-84.
41. Moy, A. B., J. Van Engelenhoven, J. Bodmer, J. Kamath, C. Keese, I. Giaever, S. Shasby, and D. M. Shasby. 1996. Histamine and thrombin modulate endothelial focal adhesion through centripetal and centrifugal forces. J. Clin. Invest. 97:1020-1027.
42. Werth, D. K., and I. Pastan. 1984, Vinculin phosphorylation in response to calcium and phorbol esters in intact cells. J. Biol. Chem. 259:5264-5270.
43. Litchfield, D. W., and E. H. Ball. 1986. Phosphorylation of the cyloskeletal protein talin by protein kinase C. Biochem. Biophys. Res. Commun. 134: 1276-1283.
44. Lynch, J. J., T. J. Ferro, F. A. Blumenstock, A. M. Brockenauer, and A. B. Malik. 1990. Increased endothelial albumin permeability mediated by protein kinase C activation. J. Clin. Invest. 85:1991-1998.
45. Turner, C. E., F. M. Pavalko, and K. Burridge. 1989. The role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity. J. Biol. Chem. 264:11938-11944.
46. Qwarnstrom, E. E., S. A. MacFarlane, R. C. Page, and S. K. Dower. 1991. Interleukin 1 beta induces rapid phosphorylation and redistribution of talin: a possible mechanism for modulation of fibroblast focal adhesion. Proc. Natl. Acad. Sci. USA 88:1232-1236.
47. Beckerle, M. C. 1990. The adhesion plaque protein, talin, is phosphorylated in vivo in chicken embryo fibroblasts exposed to a tumor-promoting phorbol ester. Cell Regul. 1:227-236.
48. Hagmann, J., and M. M. Burger. 1992. Phosphorylation of vinculin in human platelets spreading on a solid surface. J. Cell. Biochem. 50:237-244.
49. Bertagnolli, M. E., S. J. Locke, M. E. Hensler, P. F. Bray, and M. C. Beckerle. 1993. Talin distribution and phosphorylation in thrombin-activated platelets. J. Cell Sci. 106:1189-1199.
50. Bruin, T., G. M. Asijee, A. Prins, J. W. ten Cate, and A. Sturk. 1991. Subcellular distribution and phosphorylation of vinculin isoforms in human blood platelets. Thromb. Haemostasis 65:206-211.
51. Liebl, E. C., and G. S. Martin. 1992. Intracellular targeting of pp60src expression: localization of v-src to adhesion plaques is sufficient to transform chicken embryo fibrohlasts. Oncogene 7:2417-2428.
52. Burridge, K., C. E. Turner, and L. H. Romer. 1992. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119:893-903.
53. Partridge, C. A., C. J. Horvath, P. J. Del Vecchio, P. G. Phillips, and A. B. Malik. 1992. Influence of extracellular matrix in tumor necrosis factor-induced increase in endothelial permeability. Ant. J. Physiol. 263:L627-L633.
54. Whealley, E. M., P. J. McKeown-Longo, P. A. Vincent, and T. M. Saba. 1993. Incorporation of fibronectin into matrix decreases TNF-induced increase in endothelial monolayer permeability. Am. J. Physiol. 265:L148-L157.
55. Seufferlein, R., and E. Rozengurt. 1994. Lysophosphatidic acid stimulates tyrosine phosphorylation of focal adhesion kinase, paxillin, and p130: signaling pathways and cross-talk with platelet-derived growth factor. J. Biol. Chem. 269:9345-9351.
56. Rankin, S., and E. Rozengurt. 1994. Platelet-derived growth factor modulation of focal adhesion kinase (p125FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells: bell-shaped dose response and cross-talk with bombesin. J. Biol. Chem. 269:704-710.
57. Sinnett-Smith, J., I. Sachary, A. M. Valverde, and E. Rozengurt. 1993. Bombesin stimulation of p125 focal adhesion kinase tyrosine phosphorylation: role of protein kinase C, Ca2+ mobilization, and the actin cytoskeleton. J. Biol. Chem. 268:14261-14268.