Phosphorylation of inhibitory subunit of troponin and phospholamban in rat cardiomyocytes: Modulation by exposure of cardiomyocytes to hydroxyl radicals and sulfhydryl group reagents

Molecular and Cellular Biochemistry

Volumn 175, Issue 1-2, 1997, Pages 99-107

  Sulakhe, Prakash V ^a   Vo, Xuan T ^a   Phan, Thanh D ^a   Morris, Thomas E ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Cardiomyocytes; Hydroxyl radical; Inhibitory subunit of troponin; Oxygen free radicals; Phospholamban; Protein phosphorylation

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); ADENOSINE TRIPHOSPHATE; ADENYLATE CYCLASE; BETA ADRENERGIC RECEPTOR; CYCLIC AMP; FERROUS CHLORIDE; FORSKOLIN; HYDROGEN PEROXIDE; HYDROXYL RADICAL; ISOPRENALINE; N ETHYLMALEIMIDE; PHOSPHOLAMBAN; PROTEIN SUBUNIT; THIOL GROUP; TROPONIN; ADENOSINE TRIPHOSPHATASE; CALCIUM BINDING PROTEIN; FREE RADICAL; THIOL DERIVATIVE; THIOL REAGENT; TROPONIN I;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; HEART MUSCLE CELL; MALE; NONHUMAN; PROTEIN PHOSPHORYLATION; RAT; ANIMAL; AUTORADIOGRAPHY; CELL CULTURE; CYTOLOGY; DRUG EFFECT; HEART VENTRICLE; METABOLISM; OXIDATION REDUCTION REACTION; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCINTILLATION COUNTING;

ANIMALIA;

ADENOSINE TRIPHOSPHATASES; ANIMALS; AUTORADIOGRAPHY; CALCIUM-BINDING PROTEINS; CELLS, CULTURED; CYCLIC AMP; DITHIONITROBENZOIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ETHYLMALEIMIDE; FORSKOLIN; FREE RADICALS; HEART VENTRICLES; HYDROXYL RADICAL; ISOPROTERENOL; MALE; OXIDATION-REDUCTION; PHOSPHORYLATION; RATS; SCINTILLATION COUNTING; SULFHYDRYL COMPOUNDS; SULFHYDRYL REAGENTS; TROPONIN I;

EID: 0031252973     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006860110618     Document Type: Article

References (44)

1. 2+ current. Nature 298: 576-578,1982
2. 2+ influx in myocardial cells by beta adrenergic receptors cyclic nucleotides and phosphorylation. Mol Cell Biochem 82: 10-28,1988
3. Tada M, Kirchberger MA, Repke DI, Katz AM: The stimulation of calcium transport in cardiac sarcoplasmic reticulum by adenosine 3′:5′-monophosphate-dependent protein kinase. J Biol Chem 249: 6174-6180,1974
4. Tada M, Katz AM: Phosphorylation of the sarcoplasmic reticulum and sarcolemma. Ann Rev Physiol 44: 401-423,1982
5. Ray KP, England PJ: Phosphorylation of the inhibitory subunit of troponin and its effect on the calcium dependence of cardiac myofibril adenosine triphosphatase. FEBS Lett 70: 11-16,1976
6. 2+-regulatory site of bovine cardiac troponin. J Biol Chem 257: 260-263,1982
7. Katz AM, Tada M, Kirchherger MA: Control of calcium transport in the myocardium by the cyclic AMP-protein kinase system. Adv Cyclic Nucleotide Res 5: 453-472,1975
8. Zhang R, Zhao J, Mandveno A, Potter JD: Cardiac troponin I phosphorylation increases the rate of cardiac muscle relaxation. Circ Res 76: 1028-1035,1995
9. Sulakhe PV, Jagadeesh G, Braun AP: In: H Rupp (ed). The Regulation of Heart Function. George Thieme Verlag, Stuttgart, 1986 pp 71-94
10. Burton KP: Evidence of direct toxic effects of free radicals on the myocardium. Free Rad Biol Med 4: 15-24,1988
11. Kloner RA: Introduction to the role of oxygen radicals in myocardial ischemia and infarction. Free Rad Biol Med 4: 5-7,1988
12. Singal PK, Dhalla AK, Hill M, Thomas TP: Endogenous antioxidant changes in the myocardium in response to acute and chronic stress conditions. Mol Cell Biochem 129: 179-186,1993
13. Zweier JL, Flaherty JT, Weisfeldt ML: Direct measurement of free radical generation following reperfusion of ischemic myocardium. Proc Natl Acad Sci USA 84: 1404-1407,1987
14. Maupoil V, Rochelte L: Evaluation of free radicals and lipid peroxide formation during global ischemia and reperfusion in isolated perfused rat heart. Cardiovasc Drugs Ther 2: 615-621,1988
15. Prasad K, Kalra J, Chan WP, Chaudhary AK: Effects of oxygen free radicals on cardiovascular function at organ and cellular levels. Am Heart J 117: 1196-1202,1989
16. Prasad K, Kalra J, Chaudhary AK, Debnath D: Effects of polymorphonuclear leukocyte-derived oxygen free radicals and hypochlorous acid on cardiac function and some biochemical parameters. Am Heart J 119: 538-550,1990
17. Hess ML, Okabe E, Kontos HA: Proton and free oxygen radical interaction with the calcium transport system of cardiac sarcoplasmic reticulum. J Mol Cell Cardiol 13: 767-772,1981
18. Okabe E, Hess ML, Oyama M, Ito H: Characterization of free radical-mediated damage of canine cardiac sarcoplasmic reticulum. Arch Biochem Biophys 225: 164-177,1983
19. 2+-ATPase activity of cardiac sarcoplasmic reticulum. Arch Biochem Biophys 261: 447-457,1988
20. 2+-pump activity by oxygen free radicals. Am J Physiol 256: H368-H374,1989
21. Kaneko M, Matsumoto Y, Hayashi H, Kobayashi A, Yamazaki N: Oxygen free radicals and calcium homeostasis in the heart. Mol Cell Biochem 139: 91-100,1994
22. Kryski A Jr, Kenno KA, Severson DL: Stimulation of lipolysis in rat heart myocytes by isoproterenol. Am J Physiol 248: H208-H216,1985
23. Sulakhe PV, Vo XT: Regulation of phospholamban and troponin-I phosphorylation in the intact rat cardiomyocytes by adrenergic and cholinergic stimuli: roles of cyclic nucleotides, calcium, protein kinases and phosphatases, and depolarization. Mol Cell Biochem 149/150: 103-126,1995
24. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685,1970
25. Tishler PV, Epstein CJ: A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry. Anal Biochem 22: 89-98,1968
26. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin reagent. J Biol Chem 193: 265-275,1950
27. England PJ, Shahid M: Effects of forskolin on contractile responses and protein phosphorylation in the isolated perfused rat heart. Biochem J 246: 687-695,1987
28. Garvey JL, Kranias EG, Solaro RJ: Phosphorylation of C-protein, troponin I and phospholamban in isolated rabbit hearts. Biochem J 249: 709-714,1988
29. Swiderek K, Jaquet K, Meyer HE, Heilmeyer LM Jr: Cardiac troponin I, isolated from bovine heart, contains two adjacent phosphoserines. A first example of phosphoserine determination by derivatization to S-ethylcysteine. Eur J Biochem 176: 335-342,1988
30. Onorato JJ, Rudolph SA: Regulation of protein phosphorylation by inotropic agents in isolated rat myocardial cells. J Biol Chem 256: 10697-10703,1981
31. Blackshear PJ, Nemenoff RA, Bonventre JV, Cheung JY, Avruch J: Hormonal regulation of protein phosphorylation in isolated rat heart cells. Am J Physiol 246: C439-C449,1984
32. Seamon KB, Daly JW: Forskolin: a unique diterpene activator of cyclic AMP-generating systems. J Cyclic Nucl Res 7: 201-224,1981
33. Colyer J: Control of the calcium pump of cardiac sarcoplasmic reticulum. A specific role for pentameric structure of phospholamban? Cardiovascular Res 27: 1766-1771,1993
34. 2+/calmodulin-dependent protein kinase. J Biol Chem 268: 8394-8397,1993
35. Hayes JS, Brunton LL, Mayer SE: Selective activation of particulate cAMP-dependent protein kinase by isoproterenol and prostaglandin E1. J Biol Chem 255: 5113-5119,1980
36. Bode DC, Brunton LL: Post-receptor modulation of the effects of cyclic AMP in isolated cardiac myocytes. Mol Cell Biochem 82: 13-18,1988
37. 2+-ATPase of the sarcoplasmic reticulum. J Biol Chem 253: 6801-6807,1978
38. Leszyk J, Dumaswala R, Potter JD, Collins JH: Amino acid sequence of bovine cardiac troponin I. Biochemistry 27: 2821-2827,1988
39. Horwitz J, Bullard B, Mercola D: Interaction of troponin subunits. The interaction between the inhibitory and tropomyosin-binding subunits. J Biol Chem 254: 350-355,1979
40. Potter JD, Holroyde MJ, Robertson SP, Solaro RJ, Kranias EG, Johnson JD: In: RM Dowben, JW Shay (eds). Cell and Muscle Motility, vol. 2., Plenum Publishing, New York, 1982, pp 245-255
41. Casey PJ, Gilman AG: G protein involvement in receptor-effector coupling. J Biol Chem 263: 2577-2580,1988
42. Jagadeesh G, Sulakhe PV: Autonomic regulation of rat heart adenylate cyclase. Proc Can Fed Biol Soc (abstract) 27: 56,1984
43. Ross EM, Hewlett AC, Ferguson KM, Gilman AG: Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme. J Biol Chem 253: 6401-6412,1978
44. Drummond GI: Inactivation of cardiac adenylate cyclase by oxidation, trivalent arsenicals, and N-ethylmaleimide. Arch Biochem Biophys 211: 30-38,1981