Duct Epithelial Cells Cultured from Human Pancreas Processed for Transplantation Retain Differentiated Ductal Characteristics

Pancreas

Volumn 15, Issue 3, 1997, Pages 265-271

  Kolar, Carol ^a   Caffrey, Thomas ^c   Hollingsworth, Michael ^b   Scheetz, Mark ^a   Sutherlin, Marie ^a   Weide, Lamont ^c   Lawson, Terence ^b  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b Department of Surgery ^*  (United States)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

CFTR; Cytokeratin; Human; Pancreas duct epithelium; Tissue culture

Indexed keywords

CA 15-3 ANTIGEN; CARBONATE DEHYDRATASE; INSULIN; KERATIN; MESSENGER RNA; MONOCLONAL ANTIBODY; MUCIN; RNA DIRECTED DNA POLYMERASE;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL SEPARATION; CHEMISTRY; CYTOLOGY; EPITHELIUM CELL; HUMAN; IMMUNOHISTOCHEMISTRY; METHODOLOGY; PANCREAS; PANCREAS DUCT; PANCREAS TRANSPLANTATION; POLYMERASE CHAIN REACTION;

ANTIBODIES, MONOCLONAL; CA-15-3 ANTIGEN; CARBONIC ANHYDRASES; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; EPITHELIAL CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; INSULIN; KERATINS; MUCINS; PANCREAS; PANCREAS TRANSPLANTATION; PANCREATIC DUCTS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE;

EID: 0031252889     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006676-199710000-00008     Document Type: Article

References (22)

1. Hootman SR, de Ondarza J. Overview of pancreatic duct physiology and pathophysiology. Digestion 1993;54:323-30.
2. Heiman T, Githens SR. Rat pancreatic duct expiants cultured in serum-free medium on a porous support coated with an extracellular matrix. Pancreas 1991;6:514-21.
3. Githens S, Patke CL, Schexnayder JA. Isolation and culture of rhesus monkey pancreatic ductules and ductule-Iike epithelium. Pancreas 1994;9:20-31.
4. Schussler MH, Skoudy A, Ramaekers F, Real FX. Intermediate filaments as differentiation markers of normal pancreas and pancreas cancer. Am J Patliol 1992;140:559-68.
5. Batra SK, Kem HF, Worlock AJ, Metzgar RS, Hollingsworth MA. Transfection of the human MUC1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc-1: integration, expression and ultrastructural changes. J Cell Sci 1991;100:841-9.
6. Metzgar RS, Gaillard MT, Levine SJ, Tuck FL, Bossen EH, Borowitz MJ. Antigens to human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res 1982;42: 601-8.
7. Okazaki K, Tamura S, Morita M, Nishimori I, Yamamoto Y, Yamamoto Y. Interspecies crossreactive antigen of the pancreas duct cell prepared by monoclonal antibody. Int J Pancreatol 1989;5: 359-77.
8. Githens S, Schexnayder JA, Desai K, Patke CL. Rat pancreatic interlobular duct epithelium: isolation and culture in collagen gel. In Vitro Cell Exp Blol 1989;25:679-88.
9. Harris A. Epithelial cell culture. In: Harris A, ed. Tlie pancreatic duct epithelial cell. Cambridge: Press Syndicate of the University of Cambridge, 1996:42-57.
10. Ricordi C, Lacy PE, Finke EH, Olack BJ, Scharp D\V. Automated method for isolation of human pancreatic islets. Diabetes 1988;37: 413-20.
11. Chirgwin JM, Pryzbla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979;18:5294-9.
12. Lawson T, Kolar C. Mutation of V79 cells by N-dialkylnitrosamines after activation by hamster pancreas duct cells. Mutât Res 1992;272:139-44.
13. Lawson T, Kolar C. Mutagenicity of heterocyclic amines when activated by pancreas tissue. Mutât Res 1994;325:125-8.
14. Githens SR, Holmquist DRG, Whelan JF, Ruby JR. Characterization of ducts isolated from the pancreas of the rat. J Cell Biol 1980;85:122-35.
15. Hall PA, Lemoine NR. Rapid acinar to ductal transdifferentiation in cultured human exocrine pancreas. J Palhol 1992;166:97-103.
16. Vila MR, Lloreta J, Real FX. Normal human pancreas cultures display functional ductal characteristics. Lab Invest 1994;71:423-31.
17. Trautmann B, Schutt H-J, Hahn EG, Löhr M. Isolation, culture and characterization of human pancreatic duct cells. Pancreas 1993;8: 248-54.
18. Oliver C, Waters JF, Tolbert CL, Kleinman HK. Growth of exocrine acinar cells on a reconstituted basement membrane gel. In Vitro Cell Dev Biol 1987;23:465-73.
19. Taylor-Papadimitriou J, Purkis P, Fentman IS. Cholera toxin and analogues of cyclic AMP stimulate the growth of cultured human mammary epithelial cells. J Cell Physiol 1980; 102:317-21.
20. Hordijk PK, Verlaan I, Jalink K, van Coveren L-J, Moolenaar WH. cAMP abrogates the p21 raj-mitogen-activated protein kinase pathway in fibroblasts. J Biol Chem 1994;269:3534-8.
21. Quinlan RA, Schiller DL, Hatzfeld M, et al. Patterns of expression and organization of cytokeratin intermediate filaments Ann NY Acad Sci 1985:455:282-306.
22. Lawson T, Kolar C, Reyes T. Mutagenicity of 2-amino-l-methyl6-phenylimidazo[4,5-b]pyridine (PhIP) when activated by hamster pancreatic duct epithelial cells: a chemopreventive role for glutathione. Mutât Res 1997;375:73-8.