Differentiation of cultured neuroblastoma induced by staurosporine and cyclic AMP: Methods for assessing a neuronal phenotype

Brain Research Protocols

Volumn 1, Issue 4, 1997, Pages 399-405

  Sasaki, Kazuki ^a   Maruyama, Kouji ^a   Nishimura, Eiji ^a   Tsukada, Toshihiko ^a   Yamaguchi, Ken ^a  

^a NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

Cell culture; Cyclic AMP; Differentiation, in vitro; Neuroblastoma; Staurosporine

Indexed keywords

CYCLIC AMP; STAUROSPORINE;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE CELL NETWORK; NERVE FIBER GROWTH; NEUROBLASTOMA CELL; NEUROTROPISM; PHENOTYPE; PRIORITY JOURNAL;

CELL DIFFERENTIATION; CELL DIVISION; CYCLIC AMP; HUMANS; NEUROBLASTOMA; NEUROLOGY; NEURONS; PHENOTYPE; POLYMERASE CHAIN REACTION; RNA, MESSENGER; STAUROSPORINE; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 0031251797     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00016-0     Document Type: Article

References (18)

1. [1] S.R. Adams, A.T. Harootunian, Y.J. Buechler, S.S. Taylor, R.Y. Tsien, Fluorescence ratio imaging of cyclic AMP in single cells, Nature 349 (1991) 694-697.
2. [2] S.L. Berger, A.R. Kimmel (Eds.), Guide to molecular cloning techniques, Methods Enzymol. 152 (1987) 33-48.
3. [3] A. Celis, J.E. Celis, General procedures for tissue culture, in: J.E. Celis (Ed.), Cell Biology: a Laboratory Handbook, Vol. 1, Academic Press, San Diego, CA, 1994, pp. 5-17.
4. [4] P. Chomczynski, N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem. 162 (1987) 156-159.
5. [5] H.A. Erlich (Ed.), PCR Technology. Principles and Applications for DNA Amplification, Stockton Press, New York, 1989, pp. 1-16.
6. [6] M.A. Glicksman, J.E. Prantner, S.L. Meyer, M.E. Forbes, M. Dasgupta, M.E. Lewis, N. Neff, K-252a and staurosporine promote choline acetyltransferase activity in rat spinal cord cultures, J. Neurochem. 61 (1993) 210-221.
7. [7] D. Gurwitz, D.D. Cunningham, Thrombin modulates and reverses neuroblastoma neurite outgrowth, Proc. Natl. Acad. Sci. USA 85 (1988) 3440-3444.
8. [8] K. Jalink, E.J. Van Corven, T. Hengeveld, N. Morii, S. Narumiya, W.H. Moolenaar, Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho, J. Cell Biol. 126 (1994) 801-810.
9. [9] R. Makino, T. Sekiya, K. Hayashi, Evaluation of quantitative detection of mRNA by the reverse transcription-polymerase chain reaction, Technique 2 (1990) 295-301.
10. [10] E. Nishimura, K. Sasaki, T. Tsukada, K. Maruyama, K. Yamaguchi, Decrease in NRSF mRNA levels during differentiation of cultured neuroblastoma cells, Neurosci. Lett. 211 (1996) 101-104.
11. [11] S. Sakakibara, T. Imai, K. Hamaguchi, M. Okabe, J. Aruga, K. Nakajima, D. Yasutomi, T. Nagata, Y. Kurihara, S. Uesugi, T. Miyata, M. Ogawa, K. Mikoshiba, H. Okano, Mouse-Musashi-1, a neural RNA-binding protein highly enriched in the mammalian CNS stem cell, Dev. Biol. 176 (1996) 230-242.
12. [12] J. Sambrook, E.F. Fritsch, T. Maniatis (Eds.), Molecular Cloning. A Laboratory Manual, Vol. 1, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
13. [13] K. Sasaki, I. Adachi, N. Yanaihara, K. Yamaguchi, Neuronal cell differentiation of human neuroblastoma NB-OK-1 cells by staurosporine plus pituitary adenylate cyclase-activating peptide-38, Biomed. Res. 15 (Suppl. 2) (1994) 247-252.
14. [14] K. Sasaki, T. Tsukada, I. Adachi, K. Yamaguchi, Staurosporine potentiates cAMP-mediated promoter activity of the vasoactive intestinal polypeptide gene in rat pheochromocytoma PC12 cells, Biochem. Biophys. Res. Commun. 214 (1995) 1114-1120.
15. [15] K. Sasaki, T. Tsukada, K. Maruyama, K. Yamaguchi, Long-term regulation of synapsin I gene expression and neuronal morphology by cyclic AMP and low-dose staurosporine, Mol. Brain Res. 40 (1996) 157-160.
16. [16] C.J. Schoenherr, D.J. Anderson, The neuron-restrictive silencer factor (NRSF): a coordinate represser of multiple neuron-specific genes, Science 267 (1995) 1360-1363.
17. [17] A.M. Wang, M.V. Doyle, D.F. Mark, Quantitation of mRNA by the polymerase chain reaction, Proc. Natl. Acad. Sci. USA 86 (1989) 9717-9721.
18. [18] N. Yanaihara, T. Suzuki, H. Sato, M. Hoshino, Y. Okaru, C. Yanaihara, Dibutyryl cAMP stimulation of production and release of VIP-like immunoreactivity in a human neuroblastoma cell line, Biomed. Res. 2 (1981) 728-734.