An Effective Method of Completely Removing Contaminating Genomic DNA from an RNA Sample to be Used for PCR

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 8, Issue 2, 1997, Pages 135-137

  Sanyal, Arunik ^b   O'Driscoll, Shawn W ^b   Bolander, Mark E ^b   Sarkar, Gobinda ^a  

^a MAYO CLINIC   (United States)

^b NONE

Author keywords

Decontamination; DNase I; Genomic DNA; PCR; RNA

Indexed keywords

COMPLEMENTARY DNA; DEOXYRIBONUCLEASE I; DNA; EDETIC ACID; RNA;

ARTICLE; BIOCHEMISTRY; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION;

BIOCHEMISTRY; DEOXYRIBONUCLEASE I; DNA; DNA, COMPLEMENTARY; EDETIC ACID; POLYMERASE CHAIN REACTION; RNA;

EID: 0031242466     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02752257     Document Type: Article

References (5)

1. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
2. Wang, A. M., Doyle, M. V., and Mark, D. F. (1989) Quantitation of mRNA by polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86, 9717-9721.
3. Gilliland, G., Perrin, S., Blanchard, K., and Bunn, H. F. (1990) Analysis of cytokines DNA and RNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87, 2725-2729.
4. Sarkar, G. and Sommer, S. S. (1989) Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity. Science 244, 331-334.
5. Anderson, S. (1981) Shotgun DNA sequencing using cloned DNAase I-generated fragments. Nucleic Acids. Res. 9, 3015-3027.