Two-stage isolation procedure for obtaining homogenous populations of microvascular endothelial and mesothelial cells from human omentum

Microvascular Research

Volumn 54, Issue 2, 1997, Pages 121-134

  Chung Welch, Nancy ^a   Patton, Wayne F ^a   Shepro, David ^a   Cambria, Richard P ^b  

^a BOSTON UNIVERSITY   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CULTURE; CELL ISOLATION; CELL POPULATION; CELL STRUCTURE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MESOTHELIUM CELL; MICROVASCULARIZATION; OMENTUM; PHENOTYPE; PRIORITY JOURNAL; VASCULAR ENDOTHELIUM;

ULEX;

EID: 0031239615     PISSN: 00262862     EISSN: None     Source Type: Journal    
DOI: 10.1006/mvre.1997.2039     Document Type: Article

References (47)

1. Anders E., Alles J. U., Delvos U., Potzsch B., Preissner K. T., Muller-Berghaus G. Microvascular endothelial cells from human omental tissue: Modified method for long-term cultivation and new aspects of characterization. Microvasc. Res. 34:1987;239-249.
2. Chung-Welch N., Patton W., Yen-Patton G., Hechtman H., Shepro D. Phenotypic comparison between mesothelial and microvascular endothelial cell lineages using conventional endothelial cell markers, cytoskeletal protein markers andin vitro. Differentiation. 42:1989;44-53.
3. 2. J. Cell. Physiol. 135:1988;224-234.
4. Cicila G. T., O'Connell T. M., Hahn W. C., Rheinwald J. G. Cloned cDNA sequence for the human mesothelial protein "mesosecrin" discloses its identity as a plasminogen activator inhibitor (PAI-1) and a recent evolutionary change in transcript processing. J. Cell Sci. 94:1989;1-10.
5. Connell N. D., Rheinwald J. G. Regulation of the cytoskeleton in mesothelial cells: reversible loss of keratin and increase in vimentin during rapid growth in culture. Cell. 34:1983;245-253.
6. Dodge A., Patton W., Yoon M., Hechtman H., Shepro D. Organ and species specific differences in intermediate filament protein profiles of cultured microvascular endothelial cells. J. Comp. Biochem. Physiol. 98:1989;461-470.
7. Folkman J., Haudenschild C. C., Zetter B. R. Long-term culture of capillary endothelial cells. Proc. Natl. Acad. Sci. USA. 76:1979;5217-5221.
8. Fukuda K., Imamura Y., Koshihara Y., Ooyama T., Hanamure Y., Ohyama M. Establishment of human mucosal microvascular endothelial cells form inferior turbinate in culture. Am. J. Otolaryngol. 10:1989;85-91.
9. Garrels J., Franza B. The ref52 protein database: Methods of database construction and analysis using the quest system and characterizations of protein patterns from proliferating and quiescent ref52 cells. J. Biol. Chem. 264:1989;5283-5298.
10. Hewett P. W., Murray J. C. Human microvessel endothelial cells: Isolation, culture and characterization. In Vitro Cell. Dev. Biol. 29A:1993;823-830.
11. Holthofer H., Virtanen I., Kariniemi A.-L., Hormia M., Linder E., Miettinen A. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab. Invest. 47:1982;60-66.
12. Hormia M., Lehto V. P., Virtanen I. Identification of UEA I-binding surface glycoproteins of cultured human endothelial cells. Cell Biol. Int. Rep. 7:1983;467-475.
13. Jackson C. J., Garbett P. K., Nissen B., Schreiber L. Binding of human endothelium to Ulex europaeus-I coated dyna-beads: Application to the isolation of microvascular endothelium. J. Cell Sci. 96:1990;257-262.
14. Jahn L., Fouquet B., Robe K., Franke W. Cytokeratins in certain endothelial and smooth muscle cells of two taxonomically distant vertebrate species,Xenopus laevis. Differentiation. 36:1987;234-254.
15. Kern P. A., Knedler A., Eckel R. H. Isolation and culture of microvascular endothelium from human adipose tissue. J. Clin. Invest. 71:1983;1822-1829.
16. Kleinman H. K., Luckenbill-Edds L., Cannon F. W., Sephel G. C. Use of extracellular matrix components for cell culture. Anal. Biochem. 166:1987;1-13.
17. Knedler A., Ham R. G. Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell. Dev. Biol. 23:1987;481-491.
18. Kubota Y., Kleinman H. K., Martin G. R., Lawley T. J. Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J. Cell Biol. 107:1988;1589-1598.
19. Kumar S., West D. C., Ager M. Heterogeneity in endothelial cells from large vessels and microvessels. Differentiation. 36:1987;57-70.
20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
21. Latron Y., Alessi M. C., George F., Anfosso F., Poncelet P., Juhan-Vague I. Characterization of epitheloid cells from human omentum: Comparison with endothelial cells from umbilical veins. Thromb. Haemostasis. 66:1991;361-367.
22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
23. Mineau-Hanschke R., Patton W. F., Hechtman H. B., Shepro D. Immunolocalization of cytokeratin 19 in bovine and human pulmonary microvascular endothelial cells in situ. Comp. Biochem. Physiol. 104:1993;313-319.
24. Moll R., Franke W., Schiller D. The catalog of human cytokeratins: Patterns of expression in normal epithelial, tumors and cultured cells. Cell. 31:1982;11-24.
25. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced sensitivity. Anal. Biochem. 117:1981;307-310.
26. O'Farrell P. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:1975;4007-4021.
27. Patton W. F., Pluskal M., Skea W., Buecker J., Lopez M., Zimmerman R., Belanger L., Hatch P. Development of a dedicated two-dimensional gel electrophoresis system that provides optimal pattern reproducibility and polypeptide resolution. BioTechniques. 8:1990;518-527.
28. Patton W. F., Yoon M. U., Alexander J. S., Chung-Welch N., Hechtman H. B., Shepro D. S. Expression of simple epithelial cytokeratins in bovine pulmonary microvascular endothelial cells. J. Cell. Physiol. 143:1990;140-149.
29. Patton W. F. Biologist's perspective on analytical imaging systems as applied to protein gel electrophoresis. J. Chromatogr. A. 698:1995;55-87.
30. Potzsch B., Grulich-Henn J., Rossing R., Wille D., Muller-Berhaus G. Identification of endothelial and mesothelial cells in human omental tissue and in omentum-derived cultured cells by specific cell markers. Lab. Invest. 63:1990;841-852.
31. Pronk A., Leguit P., Hoynck van Papendrecht A. A. G. M., Hagelen E., van Vroonhoven T. J. M. V., Verbrugh H. A. A cobblestone cell isolated from the human omentum: The mesothelial cell). isolation, identification, and growth characteristics. In Vitro Cell. Dev. Biol. 29A:1993;127-134.
32. Rheinwald J., Jorgensen J., Hahn W., Terpstra A., O'Connell T., Plummer K. Mesosecrin: A secreted glycoprotein produced in abundance by human mesothelial, endothelial and kidney epithelial cells in culture. J. Cell Biol. 104:1987;263-275.
33. Satoh K., Prescott S. Culture of mesothelial cells from bovine pericardium and characterization of their arachidonate metabolism. Biochim. Biophys. Acta. 930:1987;283-296.
34. Scott P. A. E., Bicknell R. The isolation and culture of microvascular endothelium. J. Cell Sci. 105:1993;269-273.
35. Stansby G., Shukla N., Fuller B., Hamilton G. Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin. J. Vasc. Surg. 15:1992;583-585.
36. Sterpetti A. V., Schultz R. D., Hunter W. J., Cisternino S., Fontaine M. Comparison of two techniques to isolate microvascular endothelial cells from the omentum. J. Surg. Res. 48:1990;101-106.
37. Stylianou E., Jenner L. A., Davies M., Coles G. A., Williams J. D. Isolation, culture and characterization of human peritoneal mesothelial cells. Kidney Int. 37:1990;1563-1570.
38. Takahashi K., Goto T., Mukai K., Sawasaki Y., Hata J. Cobblestone monolayer cells from human omental adipose tissue are possibly mesothelial, not endothelial. In Vitro Cell. Dev. Biol. 25:1989;109-111.
39. Takahashi K., Hata J.-I., Mukai K., Sawasaki Y. Close similarity between cultured human omental mesothelial cells and endothelial cells in cytochemical markers and plasminogen activator production. In Vitro Cell. Dev. Biol. 27:1991;542-548.
40. Thornton S. C., Mueller S. N., Levine E. M. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science. 11:1983;623-625.
41. Titani K., Kumar S., Takio K., Ericsson L. H., Wade R. D., Ashida K., Walsh K. A., Chopek M. W., Sadler J. E., Fujikawa K. Amino acid sequence of human von Willebrand Factor. Biochemistry. 25:1986;3171-3184.
42. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
43. van Hinsbergh V. W. M., Kooistra T., Scheffer M. A., van Bockel J. H., Van Muijen G. N. P. Characterization and fibrinolytic properties of human omental tissue mesothelial cells. Comparison with endothelial cells. Blood. 75:1990;1490-1497.
44. Visser M. J. T., van Bockel J. H., van Muijen G. N. P., van Hinsbergh V. W. M. Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin. J. Vasc. Surg. 13:1991;373-381.
45. Voyta J. C., Via D., Butterfield C. E., Zetter B. R. Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J. Cell Biol. 99:1984;2034-2040.
46. Wang Z., Du W., Li G., Pu L., Sharefkin J. B. Rapid cellular luminal coverage of dacron inferior vena cava prostheses in dogs by immediate seeding of autogenous endothelial cells derived from omental tissue: Results of a preliminary trial. J. Vasc. Surg. 12:1990;168-179.
47. Wu Y., Parker L., Binder N., Beckett M., Sinard J., Griffiths T., Rheinwald J. The mesothelial keratins: A new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell. 31:1982;693-703.