The position of site-directed cleavage of RNA using RNase H and 2′-O-methyl oligonucleotides is dependent on the enzyme source

RNA

Volumn 3, Issue 9, 1997, Pages 950-

  Lapham, Jon ^a   Yu, Yi Tao ^b   Shu, Mei Di ^b   Steitz, Joan A ^b   Crothers, Donald M ^a,b  

^a YALE UNIVERSITY   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Chimera; DNA

Indexed keywords

OLIGONUCLEOTIDE; RIBONUCLEASE H; RNA;

BINDING SITE; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; METABOLISM; NOTE;

BINDING SITES; OLIGONUCLEOTIDES; RIBONUCLEASE H, CALF THYMUS; RNA; SUBSTRATE SPECIFICITY;

EID: 0031230045     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Hayase Y, Inoue H, Ohtsuka E. 1990. Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2′-O-methyl oligonucleotides. Biochemistry 29:8793-8797.
2. Inoue H, Hayase Y, Iwai S, Ohtsuka E. 1987. Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H. FEBS Lett 225:327-330.
3. Lapham J, Crothers DM. 1996. RNase H cleavage for processing of in vitro transcribed RNA for NMR studies and RNA ligation. RNA 2:289-296.
4. Xu J, Lapham J, Crothers DM. 1996. Determining RNA solution structure by segmental isotopic labeling and NMR: Applications to Caenorhabditis elegans spliced leader RNA 1. Proc Natl Acad Sci USA 93:44-48.
5. Yu Yt, Shu Md, Steitz JA. 1997. A new method for detecting sites of 2′-O-methylation in RNA molecules. RNA 3:324-331.