Immunodetection and characterization of tomato endo-β1,4-glucanase cell protein in flower abscission zones

Plant Physiology

Volumn 114, Issue 4, 1997, Pages 1541-1546

  Gonzalez Bosch, Carmen ^a,b   Del Campillo, Elena ^a,c   Bennett, Alan B ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF VALENCIA   (Spain)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISSION; CELLULASE; HYDROLASE; IMMUNOANALYSIS; PLANT GROWTH; PROTEIN ANALYSIS; TOMATO;

LYCOPERSICON ESCULENTUM;

EID: 0031200863     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.114.4.1541     Document Type: Article

References (22)

1. Brummeil DA, Lashbrook CC, Bennett AB (1994) Plant endo-β1,4-glucanases: structure, properties and physiological function. Am Chem Soc Symp Ser 566: 100-129
2. del Campillo E, Bennett AB (1996) Pedicel breakstrength and cellulase gene expression during tomato flower abscission. Plant Physiol 111: 813-820
3. del Campillo E, Lewis LN (1992) Occurrence of 9.5 cellulase and other hydrolases in flower reproductive organs undergoing major cell wall disruption. Plant Physiol 99: 1015-1020
4. del Campillo E, Reid PD, Sexton R, Lewis LN (1990) Occurrence and localization of 9.5 cellulase in abscising and nonabscising tissues. Plant Cell 2: 245-254
5. Durbin ML, Sexton R, Lewis LN (1981) The use of immunological methods to study the activity of cellulase isozymes (β-l:4 glucan 4-glucan hydrolase). Plant Cell Environ 4: 67-73
6. Fischer RL, Bennett AB (1991) Role of cell wall hydrolases in fruit ripening. Annu Rev Plant Physiol Plant Mol Biol 42: 675-703
7. Gonzalez-Bosch C, Brummell DA, Bennett AB (1996) Differential expression of two endo-l,4-β-glucanase genes in pericarp and locules of wild-type and mutant tomato fruit. Plant Physiol 111: 1313-1319
8. Harlow E, Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
9. Hprton RE, Osborne DJ (1967) Senescence, abscission and cellu- lase in Phaseolus vulgaris. Nature 214: 1086-1088
10. Jensen TE; Valdovinos JG (1967) Fine structure of abscission zones. I. Abscission zones of the pedicels of tobacco and tomato flowers at anthesis. Planta 77: 298-318
11. Kalaitzis P, Koehler SM, Tucker ML (1995) Cloning of a tomato polygalacturonase expressed in abscission. Plant Mol Biol 28: 647-656
12. Leammli UK (1970) Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
13. Lashbrook CC, Gonzalez-Bosch C, Bennett AB (1994) Two divergent endo-β-l,4-glucanase genes exhibit overlapping expression in ripening fruit and abscising flowers. Plant Cell 6: 1485-1493
14. Lewis LN, Varner JE (1970) Synthesis of cellulase during abscission of Phaseolus vulgaris leaf expiants. Plant Physiol 46: 194-199
15. Reid MS (1985) Ethylene and abscission. Hortscience 20: 45-49
16. Schaffner W, Weissmann C (1973) A rapid, sensitive and specific method for the determination of protein in dilute solutions. Anal Biochem 56: 502-514
17. Sexton R, Durbin ML, Lewis LN, Thomson WW (1981) The immunocytochemical localization of 9.5 cellulase in abscission zones of bean (Phaseolus vulgaris cv Red Kidney). Protoplasma 109: 335-347
18. Sexton R, Roberts JA (1982) Cell biology of abscission. Annu Rev Plant Physiol 33: 133-162
19. Taylor JE, Tucker GA, Lasslett Y, Smith CJS, Arnold CM, Watson CF, Schuch W, Grierson D, Roberts JA (1990) Polygalacturonase expression during leaf abscission of normal and transgenic tomato plants. Planta 183: 133-138
20. Tucker GA, Schindler CB, Roberts JA (1984) Flower abscission in mutant tomato plants. Planta 160: 164-167
21. Tucker ML, Sexton R, del Campillo E, Lewis LN (1988) Bean abscission cellulase. Characterization of a cDNA clone and regulation of gene expression by ethylene and auxin. Plant Physiol 88: 1257-1262
22. Valdovinos JG, Jensen TE (1967) Fine structure of abscission zones. II. Cell wall changes in abscising pedicels of tobacco and tomato flowers. Planta 83: 295-302