Purification and characterization of tobacco pathogenesis-related protein PR-5d, an antifungal thaumatin-like protein

Plant and Cell Physiology

Volumn 38, Issue 7, 1997, Pages 783-791

  Koiwa, Hisashi ^a   Kato, Hiroaki ^a   Nakatsu, Toru ^a   Oda, Junichi ^a   Yamada, Yasuyuki ^b   Sato, Fumihiko ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Antifungal activity; Crystallization; Nicotiana tabacum cv. Samsun NN; Osmotin; Pathogenesis related protein PR 5d; Thaumatin

Indexed keywords

FUNGI; NICOTIANA TABACUM; TABACUM;

EID: 0031194325     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029236     Document Type: Article

References (35)

1. Alexander, D., Goodman, R.M., Gut-Rella, M., Glascock, C., Weymann, K., Friedrich, L., Maddox, D., Ahl-Goy, P., Luntz, T., Ward, E. and Ryal, J. (1993) Increased tolerance to two oomycete pathogens in transgenic tobbaco expressing pathogenesis-related protein 1a. Proc. Natl. Acad. Sci. USA 90: 7328-7331.
2. Batalia, M.A., Monzingo, A.F., Ernst, S., Roberts, W. and Robertus, J.D. (1996) The crystal structure of the antifungal protein zeamatin, a member of the thaumatin-like, PR-5 protein family. Nat. Struct. Biol. 3: 19-23.
3. Bol, J.F. and van Kan, J.A.L. (1988) The synthesis and possible function of virus-induced proteins in plants. Microbiol. Sci. 5: 47-52.
4. de Vos, A.M., Hatada, M., Wel, H.v.d., Krabbendam, H., Peerdeman, A.F. and Kim, S.-H. (1985) Three-dimensional structure of thaumatin I, an intensely sweet protein. Proc. Natl. Acad. Sci. USA 82: 1406-1409.
5. Grosset, J., Meyer, Y., Chartier, Y., Kauffmann, S., Legrand, M. and Fritig, B. (1990) Tobacco mesophyll protoplasts synthesize 1,3-β-glucanase, chitinase, and "osmotins" during in vitro culture. Plant Physiol. 92: 520-527.
6. Hejgaard, J., Jacobsen, S. and Svendsen, I. (1991) Two antifungal thaumatin-like proteins from barley grain, FEBS Lett. 291: 127-131.
7. Kauffmann, S., Legrand, M. and Fritig, B. (1990) Isolation and characterization of six pathogenesis-related (PR) proteins of Samsun NN tobacco. Plant Mol. Biol. 14: 381-390.
8. Kauffman, S., Legrand, M., Geoffrey, P. and Fritig, B. (1987) Biological function of 'pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-β-glucanase activity. EMBO J. 6: 3209-3212.
9. Koiwa, H., Sato, F. and Yamada, Y. (1994) Characterization of accumulation of PR-5 proteins by IEF-immunoblot analysis. Plant Cell Physiol. 35: 821-827.
10. Kyte, J. and Doolittle, R.F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105-132.
11. Laemmli, U.K. (1970) Cleavage of structual proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
12. Legrand, M., Kauffmann, S., Geoffroy, P. and Fritig, B. (1987) Biological function of pathogenesis-related proteins: four tobacco pathogenesis-related proteins are chitinases. Proc. Natl. Acad. Sci. USA 84: 6750-6754.
13. Malehorn, D.E., Borgmeyer, J.R., Smith, C.E. and Shah, D.M. (1994) Characterization and expression of an antifungal zeamatin-like protein (Zip) gene from Zea mays. Plant Physiol. 106: 1471-1481.
14. Matthews, B.W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33: 491-497.
15. Mauch, F., Mauch-Mani, B. and Boller, T. (1988) Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combination of chitinase and β-1,3-glucanase. Plant Physiol. 88: 936-942.
16. Niderman, T., Genetet, I., Bruyère, T., Gees, R., Stinzi, A., Legrand, M., Fritig, B. and Mösinger, E. (1995) Pathyogenesis-related PR-1 proteins are antifungal. Plant Physiol. 108: 17-27.
17. Ogata, C.M., Gordon, P.F., Vos, A.M.D. and Kim, S.-H. (1992) Crystal structure of a sweet tasting protein, thaumatin I, at 1.65 Å resolution. J. Mol. Biol. 228: 893-908.
18. Ponstein, A.S., Bres-Vloemans, S.A., Sela-Buurlage, M.B., van den Elzen, P.J.M., Melchers, L.S. and Cornelissen, B.J.C. (1994) A novel pathogen- and wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiol. 104: 104-118.
19. Roberts, W.K. and Selitrennikoff, C.P. (1990) Zeamatin, an antifungal protein from maize with membrene-permealizing activity. J. Gen. Microbiol. 136: 1771-1778.
20. Sato, F., Koiwa, H., Sakai, Y., Kato, N. and Yamada, Y. (1995) Synthesis and secretion of tobacco neutral PR-5 protein by transgenic tobacco and yeast. Biochem. Biophys. Res. Commun. 211: 909-913.
21. Sato, F., Takeda, S. and Yamada, Y. (1992) Accumulation of stress-proteins in cultured tobacco cells and ethylene. In Plant Tissue Culture and Gene Manupulation for Breeding and Formation of Phytochemicals. Edited by Oono, K., Hirabayashi, T., Kikuchi, S., Handa, H. and Kajihara, K. pp. 377-389. National Institute of Agrobiological Resources, Tsukuba, Japan.
22. Schlumbaum, A., Mauch, F., Vogeli, U. and Boller, T. (1986) Plant chitinases are potent inhibitors of fungal growth. Nature 324: 365-367.
23. Sela-Buurlage, M.B., Ponstein, A.S., Vloemans, S.A., Melchers, L.S., Elzen, P.J.M.V.D. and Cornelissen, B.J.C. (1993) Only specific tobacco chitinases and β-glucanases exibit antifungal activity. Plant Physiol. 101: 857-863.
24. Singh, N.K., Bracker, C.A., Hasegawa, P.M., Handa, A.K., Hermodson, M.A., Pfankoch, E., Regnier, F.E. and Bressan, R.A. (1987) Characterization of osmotin. Plant Physiol. 85: 529-536.
25. Singh, N.K., LaRosa, C., Handa, A.K., Hasegawa, P.M. and Bressan, R.A. (1987) Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc. Natl. Acad. Sci. USA 84: 739-743.
26. Takeda, S., Sato, F., Ida, K. and Yamada, Y. (1990) Characterization of polypeptides that accumulated in cultured Nicotiana tabacum cells. Plant Cell Physiol. 31: 215-221.
27. Takeda, S., Sato, F., Ida, K. and Yamada, Y. (1991) Nucleotide sequence of a cDNA for osmotin-like protein from cultured tobacco cells. Plant Physiol. 97: 844-846.
28. van Loon, L.C. (1985) Pathogenesis-related proteins. Plant Mol. Biol. 4: 111-116.
29. van Loon, L.C., Gerritsen, Y.A.M. and Ritter, C.E. (1987) Identification, purification, and characterization of pathogenesis-related proteins from virus-infected tobacco leaves. Plant Mol. Biol. 9: 593-609.
30. van Loon, L.C., Pierpoint, W.S., Boller, T. and Conejero, V. (1994) Recommendations for naming plant pathogenesis-related proteins. Plant Mol. Biol. Rep. 12: 245-264.
31. Vigers, A.J., Roberts, W.K. and Selitrennikoff, C.P. (1990) A new family of plant antifungal proteins. Mol. Plant-Microbe Interact. 4: 315-323.
32. Vigers, A.J., Wiedemann, S., Roberts, W.K., Legrand, M., Selitrennikoff, C.P. and Fritig, B. (1992) Thaumatin-like pathogenesis-related proteins are antifungal. Plant Sci. 83: 155-161.
33. Vogel, H. and Jähnig, F. (1986) Models for the structure of outer-membrane proteins of Escherichia coli derived from Raman spectroscopy and prediction methods. J. Mol. Biol. 190: 191-199.
34. Vu, L. and Huynh, K. (1994) Isolation and characterization of a 27-kDa antifungal protein from the fruits of Diospyros texana. Biochem. Biophys. Res. Commun. 202: 666-672.
35. Woloshuk, C.P., Meulenhoff, J.S., Sela-Buurlage, M., van den Elzen, P.J.M. and Cornelissen, B.J.C. (1991) Pathogen-induced proteins with inhibitory activity toward Phytophtora infestans. Plant Cell 3: 619-628.