Purification, characterization, and kinetics of porcine recombinant dihydropyrimidine dehydrogenase

Protein Expression and Purification

Volumn 10, Issue 2, 1997, Pages 185-191

  Rosenbaum, Katrin ^a   Schaffrath, Barbara ^a   Hagen, Wilfred R ^b   Jahnke, Karin ^a   Gonzalez, Frank J ^c   Cook, Paul F ^d   Schnackerz, Klaus D ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c NATIONAL CANCER INSTITUTE   (United States)

^d UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ESCHERICHIA COLI; SUIDAE; SUS SCROFA;

EID: 0031194248     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0735     Document Type: Article

References (19)

1. Wasternack C. Degradation of pyrimidines and pyrimidine analogs - Pathways and mutual influences. Pharmacol. Ther. 8:1980;629-651.
2. 19. Cancer Res. 48:1988;1680-1688.
3. Okeda R., Shibutani M., Matsuo T., Kuroiwa T., Shimokawa R., Tajima T. Experimental neurotoxicity of 5-fluorouracil and its derivatives is due to poisoning by the monofluorinated organic metabolites, monofluoroacetic acid and α-fluoro-β-alanine. Acta Neuropathol. 81:1990;66-73.
4. Ho D. H., Towensend L., Luna M. A., Bodely G. P. Distribution and inhibition of dihydrouracil dehydrogenase activities in human tissues using 5-fluorouracil as a substrate. Anticancer Res. 6:1986;781-784.
5. Shiotani T., Weber G. Purification and properties of dihydrothymine dehydrogenase from rat liver. J. Biol. Chem. 256:1981;219-224.
6. Podschun B., Wahler G., Schnackerz K. D. Purification and characterization of dihydropyrimidine dehydrogenase from pig liver. Eur. J. Biochem. 185:1989;219-224.
7. Porter D. J. T., Chestnut W. G., Taylor L. E. C., Merrill B. M., Spector T. Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil. J. Biol. Chem. 266:1991;19988-19994.
8. Lu Z. H., Zhang R., Diasio R. Purification and characterization of dihydropyrimidine dehydrogenase from human liver. J. Biol. Chem. 267:1992;17102-17109.
9. Podschun B. Cook. P. F., Schnackerz K. D. Kinetic mechanism of dihydropyrimidine dehydrogenase from pig liver. J. Biol. Chem. 265:1990;12966-12972.
10. Podschun B. Stereochemistry of NADPH oxidation by dihydropyrimidine dehydrogenase from pig liver. Biochem. Biophys. Res. Commun. 182:1992;609-616.
11. Gani D., Young D. W. Stereochemistry of catabolism of the RNA base uracil. J. Chem. Soc. Perkin Trans. I. 1985;1355-1362.
12. Podschun B., Jahnke K., Schnackerz K. D., Cook P. F. Acid base catalytic mechanism of the dihydropyrimidine dehydrogenase from pH studies. J. Biol. Chem. 268:1993;3407-3413.
13. Yokota H., Fernandez-Salguerno P., Furuya H., Lin K., McBride O. W., Podschun B., Schnackerz K. D., Gonzalez F. J. cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria. J. Biol. Chem. 269:1994;23192-23196.
14. Blum H., Beyer H., Gross H. J. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis. 8:1987;93-99.
15. Cleland W. W. Statistical analysis of enzyme kinetic data. Methods Enzymol. 63:1979;103-138.
16. Tatsumi K., Fukushima M., Shirasaka T., Fujii S. Inhibitory effects of pyrimidine, barbituric acid and pyridine derivatives on 5-fluorouracil degradation in rat liver extracts. Jpn. J. Cancer Res. (Gann). 78:1987;748-755.
17. Mathews R., Charlton S., Sands R. H., Palmer G. On the nature of the spin coupling between the iron-sulfur cluster in the eight-iron ferrodoxins. J. Biol. Chem. 249:1974;4326-4328.
18. Grande H. J., Dunham W. R., Averill B., van Dijk C., Sands R. H. Electron paramagnetic resonance and other properties of hydrogenases isolated from Desulfovibrio vulgaris (strain Hildenborough) and Megasphaera elsdenii. Eur. J. Biochem. 136:1983;201-207.
19. Jetten M. S. M., Hagen W. R., Pierik A. J., Stams A. J. M., Zehnder A. J. B. Paramagnetic centers and acetyl-coenzyme A/CO exchange activity of carbon monoxide dehydrogenase from Methanothrix soehngenii. Eur. J. Biochem. 195:1991;385-391.