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Volumn 30, Issue 14, 1997, Pages 4235-4237

Molecular assembly of cholesterol-bearing poly(allylamine) for binding bile salts in water

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM COMPOUNDS; CHEMICAL BONDS; CHOLESTEROL; COMPLEXATION; FREE RADICAL POLYMERIZATION; INFRARED SPECTROSCOPY; MOLECULAR STRUCTURE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SALTS; SYNTHESIS (CHEMICAL); TRANSMISSION ELECTRON MICROSCOPY; WATER;

EID: 0031187928     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma970116o     Document Type: Article
Times cited : (12)

References (26)
  • 5
    • 0002253711 scopus 로고
    • Interactions between Macromolecules in Solution and Intermacromolecular Complexes
    • Springer-Verlag: Berlin
    • Tsuchida, E.; Abe, K. Interactions Between Macromolecules in Solution and Intermacromolecular Complexes; Advances in Polymer Science, No. 45; Springer-Verlag: Berlin, 1982.
    • (1982) Advances in Polymer Science , vol.45
    • Tsuchida, E.1    Abe, K.2
  • 20
    • 3943106406 scopus 로고
    • 50 = 1600 mg/kg (mouse) as a hydrochloric salt. See: Harada, S. Kinouzairyou 1985, 5, 29.
    • (1985) Kinouzairyou , vol.5 , pp. 29
    • Harada, S.1
  • 21
    • 85033172835 scopus 로고    scopus 로고
    • note
    • Sodium glycocholate (GC) and sodium taurodeoxycholate were employed because they are the representative bile salts in human bile.
  • 22
    • 85033185546 scopus 로고    scopus 로고
    • note
    • Bile salt binding by the polymer was analyzed as follows. GC (10 m.M) and TDC (15 mM) solutions in 0.3 M phosphate buffer (pH = 6.0) were prepared. Samples (10 mg) of PAA-C were accurately weighed and placed into 10 mL vials together with a 4.5 mL portion of the bile salt solution. All vials were securely closed and mechanically shaken at 25 °C until equilibrium was established. Equilibration was determined by means of repetitive sampling and was found to occur within a 10 h period. The equilibrated samples were centrifuged at 12 000 rpm for 10 m, and the supernatant solution was assayed for unbound bile salt using an enzymatic reaction method with a bile salt concentration measuring reagent (3α-hydroxysteroid dehydrogenase). The amount of bile salt bound to PAA-C was calculated from the difference between the initial concentration of bile salt introduced into the system and the concentration present in the solution at equilibrium. In the dissociation studies, bile salt binding PAA-C recovered after centrifugation was placed into a 10 mL vial with 4.5 mL of 0.3 M phophate buffer (pH = 8.0). After equilibration, the solution was centrifuged at 12 000 rpm for 10 m and assayed for bile salt concentration.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.