Application of reversible biotinylated label for directed immobilization of synthetic peptides and proteins: Isolation of ligates from crude cell lysates

Journal of Peptide Science

Volumn 3, Issue 4, 1997, Pages 252-260

  Ball, H L ^a,b   Mascagni, P ^a  

^a Italfarmaco SpA   (Italy)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Affinity chromatography; Avidin biotin; Chaperonin 10 from Rattus norvegicus; Directed immobilization; GroEL; Peptide synthesis

Indexed keywords

ESCHERICHIA COLI; RATTUS NORVEGICUS;

BIOTIN; CHAPERONIN; PEPTIDE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; RAT; SYNTHESIS;

ANIMALS; BIOTIN; CHAPERONIN 10; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GROEL PROTEIN; PEPTIDES; PROTEIN BINDING; RATS;

EID: 0031184172     PISSN: 10752617     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1099-1387(199707)3:4<252::aid-psc100>3.0.co;2-4     Document Type: Article

References (20)

1. O. Larm, R. Larson and P. Olsson, in: Heparin Chemical and Clinical Applications, D. A. Lane and U. Lindahl, Eds. p. 597, CRC Press, Boca Raton, FL 1989.
2. I. Danishefsky, F. Tzeng, M. Ahrens and S. Klein (1979). Synthesis of heparin-sepharoses and their binding with thrombin and anti-thrombin-heparin cofactor. Thrombosis Res. 8, 131-140.
3. Y. Miyura, S. Asoyugi, Y. Kusada and K. Miyamoto (1980). The characteristics of anti-coagulation by covalently immobilized heparin. J. Biomed. Mater. Res. 14, 619-623.
4. C. D. Ebert and S. W. Kim (1982). Immobilized heparin: Spacer arm effects on biological interactions. Thrombosis Res. 26, 43-47.
5. V. D. Nadkarni, A. Pervin and R. J. Lindhardt (1994). Directional immobilization of heparin onto beaded supports. Anal Biochem. 222, 59-67.
6. G. Fassina, G. Cassani, P. Gnocchi, M. C. Fornasiero and A. M. Isetta (1995). Inhibition of interleukin-2/p55 receptor subunit interaction of complementary peptides. Arch. Biochem. Biophys. 318, 37-45.
7. P. Hansen, G. Lindeberg and L. Anderson (1992). Immobilized metal ion affinity chromatography of synthetic peptides. J. Chromatogr. 627, 125-135.
8. M. D. Savage, G. Mattson, S. Desai, G. W. Nielander, S. Morgensen and E. J. Conklin, Eds: Avidin-Biotin Chemistry: A Handbook, p. 273-297, Pierce Chemical Co., Rockford, IL 1992.
9. T. J. Lobl, M. R. Deibel and A. W. Yen (1988). On-resin biotinylation of chemically synthesized proteins for one-step purification. Anal. Biochem. 170, 502-511.
10. A. G. Tomasselli, C. A. Bannow, M. R. Deibel, J. O. Hui, H. A. Zurcher-Neely, I. M. Reardon, C. W. Smith and R. L. Heinrikson (1992). Chemical synthesis of a biotinylated derivative of the simian immunodeficiency virus protease. J. Biol. Chem. 267, 10232-10237.
11. S. Funakoshi, H. Funuda and N. Fujii (1993). Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-phase technique. J. Chromatogr. 638, 21-57.
12. H. L. Ball and P. Mascagni (1992). Purification of synthetic peptides using reversible chromatographic probes based on the Fmoc molecule. Int. J. Peptide Protein Res. 40, 370-379.
13. H. L. Ball, G. Bertolini, S. Levi and P. Mascagni (1994). Purification of synthetic peptides with the aid of chromatographic probes. J. Chromatogr. A 686, 73-83.
14. H. L. Ball and P. Mascagni (1995). Affinity purification of 101 residue rat cpn10 using a reversible biotinylated probe. J. Peptide Sci. 1, 288-294.
15. ras. Biochem. Biophys. Acta 1164, 219.
16. H. L. Ball and P. Mascagni (1996). Chemical synthesis of proteins. A methodology. Int. J. Peptide Protein Res., 48, 31-47.
17. H. L. Ball, P. Giuliani, P. Lucietto, G. Fossati and P. Mascagni (1995). Chemical synthesis and characterization of rat cpn10. Effect of chain length, ions, heat and N-terminal acetylation on unchaperoned folding into its heptameric form. Biomed. Peptides, Proteins Nucleic Acids 1, 39-44.
18. H. L. Ball and P. Mascagni (1995). N-(-2-chlorobenzyloxycarbonyloxy) succinimide as a terminating agent for solid-phase peptide synthesis. Application to a one-step purification procedure. Lett. Peptide Sci. 2, 49-57.
19. V. K. Sarin, S. B. H. Kent, J. P. Tam and R. B. Merrifield (1981). Quantitative monitoring of SPPS by the ninhydrin reaction. Anal. Biochem. 117, 147-157.
20. J. P. Tam (1987). Synthesis of biologically active transforming growth factor alpha. Int. J. Peptide Protein Res. 29, 421-431.