A Preparative Method for Crosslinking Proteins to DNA in Nuclei by Single-pulse UV Laser Irradiation

Photochemistry and Photobiology

Volumn 66, Issue 1, 1997, Pages 42-45

  Mutskov, Vesco ^a   Angelov, Dimitar ^b   Pashev, Iliya ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY   (Bulgaria)

^b INSTITUTE OF SOLID STATE PHYSICS   (Bulgaria)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS LINKING REAGENT; DNA; HISTONE;

ANIMAL; ARTICLE; CELL CULTURE; ERYTHROLEUKEMIA; FRIEND LEUKEMIA VIRUS; LASER; METABOLISM; MOUSE; PROTEIN BINDING; RADIATION EXPOSURE; ULTRAVIOLET RADIATION;

ANIMALS; CROSS-LINKING REAGENTS; DNA, NEOPLASM; FRIEND MURINE LEUKEMIA VIRUS; HISTONES; LASERS; LEUKEMIA, ERYTHROBLASTIC, ACUTE; MICE; PROTEIN BINDING; TUMOR CELLS, CULTURED; ULTRAVIOLET RAYS;

EID: 0031184054     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-1097.1997.tb03136.x     Document Type: Article

References (18)

1. Shetlar, M. D. (1980) Cross-linking of proteins to nucleic acids by ultraviolet light. Photochem. Photobiol. Rev. 5, 105-197.
2. Nikogosyan, D. N. (1990) Two-quantum UV photochemistry of nucleic acids: comparison with conventional low-intensity UV photochemistry and radiation chemistry. Int. J. Radiat. Biol. 97, 233-299.
3. Pashev, I. G., S. I. Dimitrov and D. Angelov (1991) Crosslinking proteins to nucleic acids by ultraviolet laser irradiation. Trends Biochem. Sci. 16, 323-326.
4. Hockensmith, J. W., W. L. Kubasek, W. R. Vorachek, E. M. Evertsz and P. H. von Hippel (1991) Laser cross-linking of protein-nucleic acid complexes. Methods Enzymol. 28, 211-236.
5. Harrison, C. A., D. Turner and D. C. Hinkle (1982) Laser crosslinking of E. coli RNA polymerase and T7 DNA. Nucleic Acids Res. 10, 2399-2414.
6. Hockensmith, J. W., W. L. Kubasek, W. R. Vorachek and P. H. von Hippel (1986) Laser cross-linking of nucleic acids to proteins. J. Biol. Chem. 261, 2512-1518.
7. Angelov, D., V. Yu. Stefanovsky, S. I. Dimitrov, V. R. Russanova, E. Keskinova and I. G. Pashev (1988) Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation. Nucleic Acids Res. 16, 4525-4538.
8. Stefanovsky, V. Yu., S. I. Dimitrov, V. R. Russanova, D. Angelov and I. G. Pashev (1989) Laser-induced crosslinking of histones to DNA in chromatin and core particles: implications in studying histone-DNA interactions. Nucleic Acids Res. 17, 10069-10081.
9. Stefanovsky, V. Y., S. I. Dimitrov, D. Angelov and I. G. Pashev (1989) Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinking. Biochem. Biophys. Res. Commun. 164, 304-310.
10. Kubasek, W. L., D. Spann and J. W. Hockensmith (1993) Laser cross-linking of proteins to nucleic acids: photodegradation and alternative photoproducts of the bacteriophage T4 gene 32 protein. Photochem. Photobiol. 58, 1-10.
11. Budowsky, E. I., M. S. Axentieva, G. G. Abdurashidova, N. A. Simukova and L. B. Rubin (1986) Induction of polynucleotide-protein cross-linkages by UV irradiation. Eur. J. Biochem. 159, 95-101.
12. Russanova, V. R., S. I. Dimitrov, V. L. Makarov and I. G. Pashev (1987) Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin. Eur. J. Biochem. 167, 321-326.
13. Gilmour, D. S. and J. T. Lis (1984) Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes. Proc. Natl. Acad. Sci. USA 81, 4275-4279.
14. Dimitrov, S. I., V. Yu. Stefanovsky, L. Karagyozov, D. Angelov and I. G. Pashev (1990) The enhancers and promoters of the Xenopus laevis ribosomal spacer are associated with histones upon active transcription of the ribosomal genes. Nucleic Acids Res. 18, 6393-6397.
15. Dimitrov, S. I., H. Tateossyan, V. Yu. Stefanovsky, V. R. Russanova, L. Karagyozov and I. G. Pashev (1992) Binding of histones to Xenopus laevis ribosomal genes with different level of expression. Eur. J. Biochem. 204, 977-981.
16. Mutskov, V. J., V. R. Russanova, S. I. Dimitrov and I. G. Pashev (1996) Histones associated with non-nucleosomal rat ribosomal genes are acetylated while those bound to nucleosome-organized gene copies are not. J. Biol. Chem. 271, 11852-11857.
17. Cao, T. M. and M. T. Sung (1982) Ultraviolet light induced cross-linking of histone H3 to deoxyribonucleic acid in chromatin and nuclei of chicken erythrocytes. Biochemistry 21, 3419-3427.
18. Graba, Y., D. Aragnol, P. Laurenti, V. Garzino, D. Charmot, H. Berenger and J. Pradel (1992) Homeotic control in Drosophila: the scabrous gene is an in vivo target of Ultrabithorax proteins. EMBO J. 11, 3375-3384.