Yeast chromatin reconstitution system using purified yeast core histones and yeast nucleosome assembly protein-1

Protein Expression and Purification

Volumn 10, Issue 1, 1997, Pages 132-140

  Pilon, John ^a   Terrell, Andrea ^a   Laybourn, Paul J ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031172308     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0716     Document Type: Article

References (37)

1. van Holde K. E., Zlatonova J., Arents G., Moudrianakis E. Elements of chromatin structure: Histones, nucleosomes, and fibres. Chromatin Structure and Gene Expression. 1995;Oxford University Press, New York. p. 1-26.
2. Wolffe A. P. Chromatin: Structure and Function. 1995;Academic Press, San Diego.
3. Bajwa W., Meyhack B., Rudolph H., Schweingruber A-M., Hinnen A. Structural analysis of the two tandemly repeated acid phosphatase genes in yeast. Nucleic Acids Res. 12:1984;7721-7739.
4. Fisher E. A., Felsenfeld G. A comparison of the forlding of beta-globin and ovalbumin gene-containing chromatin isolated from chicken oviduct and erythrocytes. Biochemistry. 25:1986;8010-8016.
5. Forrester W. C., Thompson C., Elder J. T., Groudine M. A developmentally stable chromatin structure in the human beta-globin gene cluster. Proc. Natl. Acad. Sci. USA. 83:1986;1359-1363.
6. Benezra R., Cantor C. R., Axel R. Nucleosomes are phased along the mouse beta-major globin gene in erythroid and nonerythroid cells. Cell. 44:1986;697-704.
7. Fritton H. P., Igo-Kemenes T., Nowock J., Strech-Jurk U., Theisen M., Sippel A. E. DNAase I-hypersensitive sites in the chromatin structure of the lysozyme gene in steroid hormone target and nontarget cells. Biol. Chem. Hoppe-Seyler. 368:1987;111-119.
8. Richard-Foy H., Hagar G. L. Sequence-specific positioning of nucleosomes over the steroid-inducable MMTV promoter. EMBO J. 6:1987;2321-2328.
9. Almer A., Rudolph H., Hinnen A., Hörz W. Removal of positioned nucleosomes from the yeastPHO5PHO5. EMBO J. 5:1986;2689-2696.
10. Bergman L. W., Kramer R. A. Modulation of chromatin structure associated with derepression of the acid phosphatase gene of Saccharomyces cerevisiae. J. Biol. Chem. 258:1983;7223-7227.
11. Fascher K-D., Schmitz J., Hörz W. Role of trans-activating proteins in the generation of active chromatin at thePHO5S. cerevisiae. EMBO J. 9:1990;2523-2528.
12. Straka C., Hörz W. A functional role for nucleosomes in the repression of a yeast promoter. EMBO J. 10:1991;361-368.
13. Durrin L. K., Mann R. K., Kayne P. S., Grunstein M. Yeast histone H4 N-terminal sequence is required for promoter activationin vivo. Cell. 65:1991;1023-1031.
14. Han M., Grunstein M. Nucleosome loss activates yeast downstream promotersin vivo. Cell. 55:1988;1137-1145.
15. Clark-Adams C. D., Norris D., Osley M. A., Fassler J. S., Winston F. Changes in histone gene dosage alter transcription in yeast. Genes Dev. 2:1988;150-159.
16. Norris D., Dunn B., Osley M. A. The effect of histone gene deletions on chromatin structure in Saccharomyces cerevisiae. Science. 242:1988;759-761.
17. Scott J., Schekman R. Lyticase: Endoglucanase and protease activities that act together in yeast cell lysis. J. Bacteriol. 142:1980;414-423.
18. Sambrook J., Tritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor.
19. Nossal N. G., Heppel L. A. J. Biol. Chem. 241:1966;3055-3062.
20. Sherman F. Getting started with yeast. Methods Enymol. 194:1991;3-21.
21. Lue N. F., Flanagan P. M., Kelleher R. T. III, Edwards A. M., Kornberg R. D. Methods Enzymol. 194:1991;545-550.
22. Simon R. H., Felsenfeld G. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite. Nucleic Acids Res. 6:1979;689-696.
23. Laybourn P. J., Kadonaga J. T. Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II. Science. 254:1991;238-245.
24. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227:1970;680-685.
25. Lennox R. W., Cohen L. H. Analysis of histone subtypes and their modified forms by polyacrylamide gel electrophoresis. Methods Enzymol. 170:1989;532-549.
26. Ishimi Y., Kikuchi A. Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nulceosome assemblyin vitro. J. Biol. Chem. 266:1991;7025-7029.
27. Fujii-Nakata T., Ishimi Y., Okuda A., Kikuchi A. Functional analysis of nucleosome assembly protein, NAP-1. J. Biol. Chem. 267:1992;20980-20986.
28. Goto T., Laipis P., Wang J. C. The purification and characterization of DNA topoisomerase I and II from the yeastSaccharomyces cerevisiae. J. Biol. Chem. 259:1984;10422-10429.
29. Peck L. J., Wang J. C. Energetics of B-to-Z transition in DNA. Proc. Natl. Acad. Sci. USA. 80:1983;6206-6210.
30. Shimamura A., Tremethick D., Worcel A. Characterization of the repressed 5S DNA minichromosomes assembledin vitroXenopus laevis. Mol. Cell. Biol. 8:1988;4257-4269.
31. Fukuma M., Hiraoka Y., Sakura H., Fukasawa T. Purification of yeast histones competent for nucleosome assemblyin vitro. Yeast. 10:1994;319-331.
32. Lorch Y., Kornberg R. D. Isolation of the yeast histone octamer. Proc. Natl. Acad. Sci. USA. 91:1994;11032-11034.
33. Davie J. R., Saunders C. A., Walsh J. M., Weber S. C. Histone modifications in the yeastS. cerevisiae. Nucleic Acids Res. 9:1981;3205-3215.
34. Hörz W., Zachau H. G. Deoxyrobonuclease II as a probe for chromatin structure. I. Location of cleavage sites. J. Mol. Biol. 144:1980;305-328.
35. Thomas J. O., Furber V. Yeast chromatin structure. FEBS Lett. 66:1976;274-280.
36. Tatchell K., Van Holde K. E. Reconstitution of chromatin core particles. Biochemistry. 16:1977;5295-5303.
37. Morse R. H., Pederson D. S., Dean A., Simpson R. T. Yeast nucleosomes allow thermal untwisting of DNA. Nucleic Acids Res. 15:1987;10311-10330.