Cloning and expression of a cysteine-rich venom protein from Trimeresurus mucrosquamatus (Taiwan habu)

Toxicon

Volumn 35, Issue 6, 1997, Pages 879-888

  Chang, Tseng Yuan ^a   Mao, Shou Hsian ^a   Guo, Yaw Wen ^a  

^a NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PROTEIN; SNAKE VENOM;

AMINO ACID SEQUENCE; ARTICLE; CLONING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SNAKE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CONSENSUS SEQUENCE; CROTALID VENOMS; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENETIC CODE; GENETIC VECTORS; LIZARDS; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PROTEINS; RATS; RECOMBINANT FUSION PROTEINS; REPTILIAN PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; PROTOBOTHROPS MUCROSQUAMATUS; SERPENTES; SQUAMATA; TRIMERESURUS; TRIMERESURUS FLAVOVIRIDIS;

EID: 0031172160     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(96)00192-4     Document Type: Article

References (27)

1. Burnette, W. N. (1981) 'Western blotting': electrophorelic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analyt. Biochem. 112, 195-203.
2. Charest, N. J., Joseph, O. R., Wilson, E. M. and French, F. S. (1988) Molecular cloning of complementary deoxyribonucleic acid for an androgen-regulated epididymal protein: sequence homology with metalloproteins. Molec. Endocr. 2, 999-1004.
3. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. and Rutter, W. J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
4. Cornelissen, B. J. C., Hooft van Huijsduijnen, R. A. M., Van Loon, L. C. and Bol, J. F. (1986) Molecular characterization of messenger RNAs for 'pathogenesis-related' proteins 1a, 1b and 1c, induced by TMV infection of tobacco. Eur. molec. Biol. Org. J. 5, 37-40.
5. Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem. 132, 6-13.
6. Frohman, M. A., Dush, M. K. and Martin, G. R. (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. natn. Acad. Sci. U.S.A. 85, 8998-9002.
7. Grissmer, S., Nguyen, A. N., Aiyar, J., Hanson, D. C., Mather, R. J., Gutman, G. A., Karmilowicz, M. J., Auperin, D. D. and Chandy, K. G. (1994) Pharmacological characterization of five cloned voltage-gated K channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines. Molec. Pharmac. 45, 1227-1234.
8. Haendler, B., Krätzschmar, J., Theuring, F. and Schleuning, W. D. (1993) Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland. Endocrinology 133, 192-198.
9. Huang, K. F., Hung, C. C., Pan, F. M., Chow, L. P., Tsugita, A. and Chiou, S. H. (1995) Characterization of multiple metalloproteinases with fibrinogenolytic activity from the venom of Taiwan habu (Trimeresurus mucrosquamatus): protein microsequencing coupled with cDNA sequence analysis. Biochem. biophys. Res. Commun. 216, 223-233.
10. Hung, C. C., Huang, K. F. and Chiou, S. H. (1994) Characterization of one novel venom protease with beta-fibrinogenase activity from the Taiwan habu (Trimeresurus mucrosquamatus): purification and cDNA sequence analysis. Biochem. biophys. Res. Commun. 205, 1707-1715.
11. Iha, M., Qi, Z. Q., Kannki, T., Tomihara, Y. and Yonaha, K. (1995) The primary structure of a hemorrhagic factor, HR2b, from the venom of Okinawa habu (Trimeresurus flavoviridis). Toxicon 33, 229-239.
12. Kasahara, M., Gutknecht, J., Brew, K., Spurr, N. and Goodfellow, P. N. (1989) Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene. Genomics 5, 527-534.
13. Kent, G. C. (1983) In: Comparative Anatomy of the Vertebrates, p. 314 (Kent, G. C., Ed.). St Louis. MO: C. V. Mosby.
14. Kozak, M. (1991) An analysis of vertebrate mRNA sequences: intimations of translational control. J. Cell Biol. 115, 887-903.
15. Loh, E. Y., Elliott, J. F., Cwirla, S., Lanier, L. L. and Davis, M. M. (1989) Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science, N.Y. 243, 217-220.
16. Lu, G., Villalba, M., Coscia, M. R., Hoffman, D. R. and King, T. P. (1993) Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets. J. Immun. 150, 2823-2830.
17. Lucas, J., Camacho Henriquez, A. C., Lottspeich, F., Henschen, A. and Saenger, H. L. (1985) Amino acid sequence of the 'pathogenesis-related' leaf protein P14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins. Eur. molec. Biol. Org. J. 4, 2745-2749.
18. Maniatis, T., Fristsch, E. F. and Sambrook, J. (1989) In: Molecular Cloning: A Laboratory Manual, pp. 8.3-8.80 (Ford, N., Nolan, C. and Ferguson, M., Eds). Cold Spring Harbor. NY: Cold Spring Harbor Laboratory Press.
19. Mao, S. H., Yin, F. Y. and Guo, Y. W. (1980) A comparative study of the head of Taiwanese common venomous snakes. Snake 18, 6-15.
20. Morrissette, J., Krätzschmar, J., Haendler, B., el-Hayek, R., Mochea-Morales, J., Martin, B. M., Patel, J. R., Moss, R. L., Schleuning, W. D., Coronado, R. and Possani, L. D. (1995) Primary structure and properties of helothermine, a peptide toxin that blocks ryanodine receptors. Biophys. J. 68, 2280-2288.
21. Muradov, A., Petrasovits, L., Davidson, A. and Scott, K. J. (1993) A cDNA clone for a pathogenesis-related protein 1 from barley. Plant molec. Biol. 23, 439-442.
22. Muszkat, K. A., Preygerzon, V. and Tu, A. T. (1994) CIDNP study of the aromatic side chain interactions in myotoxin alpha. J. Protein Chem. 13, 333-337.
23. Sanger, F., Nicklen, S. and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. natn. Acad. Sci. U.S.A. 74, 5463-5467.
24. Tsai, I. H., Wang, Y. M. and Lim, Y. H. (1994) Characterization of a cDNA encoding the precursor of platelet aggregation inhibition and metalloproteinase from Trimeresurus mucrosquamatus venom. Biochim. biophys. Acta 1200, 337-340.
25. Tsai, I. H., Lu, P. J., Wang, Y. M., Ho, C. I. and Liaw, L. L. (1995) Molecular cloning and characterization of a neurotoxic phospholipase A-from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. J. 311, 895-900.
26. von Heijne, G. (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur. J. Biochem. 133, 17-21.
27. Young, R. A. and Davis, R. W. (1983) Yeast RNA polymerase II genes: isolation with antibody probes. Science, N. Y. 222, 778-782.