Evaluation of a model for the detection of aerosol-containing protease in real-time using chromogenic substrates in the sampling fluid of a cyclone and a bubbler

Journal of Aerosol Science

Volumn 28, Issue 3, 1997, Pages 501-510

  Nitescu, I ^a   Cumming, R H ^a   Rowell, F J ^b   Steward, I W ^c  

^a TEESSIDE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SUNDERLAND   (United Kingdom)

^c RICARDO UK LTD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ATMOSPHERIC HUMIDITY; BUBBLE CHAMBERS; CHROMOGENICS; CONCENTRATION (PROCESS); ENZYMES; SUBSTRATES;

AEROJET CYCLONE; ALCALASE; LOW FLOW RATE BUBBLER;

ATMOSPHERIC AEROSOLS;

CHROMOGENIC SUBSTRATE; PROTEINASE; SUBTILISIN;

AEROSOL; ARTICLE; COLOR; FLOW RATE; HUMIDITY; INTERMETHOD COMPARISON; PREDICTION; PRIORITY JOURNAL; SAMPLING; TEMPERATURE;

EID: 0031128482     PISSN: 00218502     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-8502(96)00451-X     Document Type: Article

References (15)

1. Baughman, K. W. and Love, D. H. (1993) Industrial hygiene chemistry. The method development approach to air analysis. Anal. Chem. 4, 65.
2. Bradford, M. M. (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Cumming, R. H., Farrell, C., Nitescu, I., Rowell, F. J. and Tang, L. X. (1995) Environmental analysis in the workplace: development of a rapid sensitive ELISA for monitoring airborne Alcalase. Anal. Chim. Acta 311, 377-382
4. Griffiths, W. D., Upton, S. L. and Mark, D. (1993a) An investigation into the collection efficiency and bioefficiencies of a number of aerosol samplers. J. Aerosol Sci. 24, (suppl. 1), S541-S542.
5. Hambleton, P., Bennett, A. M. and Leaver, G. (1992) Biosafety monitoring devices for biotechnology processes. TIBTECH 10, 192-199.
6. Health and Safety Executive (1994) Occupational exposure limits. EH 40/94, HMSO, London.
7. Koochaki, Z., Cumming, R. H., Rowell, F. J. and Stewart, I. W. (1995) The measurement of the levels of hazardous biochemicals in the work place atmosphere using a biosensing system coupled to a personal exposure monitor. Process Biochem. 30, 589-597.
8. Nitescu, I., Cumming, R. H. and Rowell, F. J. (1996b) Suitability of chromogenic substrates for Alcalase detection for inclusion in an aerosol monitoring system. Ann. Occup. Hyg. (in press).
9. Nitescu, I., Koochaki, Z., Cumming, R. H. and Rowell, F. J. (1996c) Use of chromogenic substrates in personal monitors for the detection of protease in factory air: a theoretical model. Ann. Occup. Hyg. (in press).
10. Nitescu, I., Rowell, F. J. and Cumming, R. H. (1996a) A rapid fluorescence ELISA for subtilisin. Analy. Commun. 33, 21-22.
11. Robertson, D. S. F. (1983) Enzyme in industry. In Encyclopaedia of Occupational Health and Safety, Vol. 1 (Edited by Parmeggiani, L), International Labor Office, Geneva.
12. Stewart, I. W., Leaver, G. and Futter, S. J. (1995) The enumeration of aerosolised Saccharomyces cerevisiae using bioluminescent assay of total adenylates. J. Aerosol Sci. (This issue).
13. Tang, L. W., Rowell, F. J. and Cumming, R. H. (1995) Development of near-real-time monitoring systems for some serine protease enzymes in the industrial atmosphere. Ann. Occup. Hyg. (in press).
14. Tyler, M. E., Shipe, E. L. and Painter, R. B. (1959) Bacterial aerosol samplers: comparison of biological and physical effects in liquid impinger samplers. Appl. Microbiol. 7, 355-361.
15. Upton, S. L., Mark, D., Douglass, E. J., Hall, D. J. and Griffiths, W. D. (1994) A wind tunnel evaluation of the physical sampling efficiencies of 3 bioaerosol samplers. J Aerosol Sci. 25, 1493-1501.