Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum

Molecular and Cellular Probes

Volumn 11, Issue 2, 1997, Pages 149-153

  Newton, L A ^a   Chilton, N B ^a   Monti, J R ^a   Bjorn H ^b   Varady M ^b,c   Christensen, C M ^b   Gasser, R B ^a,b  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

Internal transcribed spacer; Oesophagostomum dentatum; Oesophagostomum quadrispinulatum; Parasitic nematodes; Ribosomal DNA; Species identification; Specific PCR

Indexed keywords

ANIMAL MODEL; ARTICLE; DEVELOPMENT; FECES; INTESTINE; LARVA; MORPHOLOGY; NONHUMAN; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; POPULATION GENETICS; PRIORITY JOURNAL; STRONGYLOIDEA; SWINE; WORM;

ANIMALIA; NEMATODA; OESOPHAGOSTOMUM DENTATUM; OESOPHAGOSTOMUM QUADRISPINULATUM; STRONGYLOIDEA; SUIDAE; SUS SCROFA;

EID: 0031127805     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1997.0097     Document Type: Article

References (17)

1. Stewart T. B., Gasbarre L. C. The veterinary importance of nodular worms (Oesophagostomum. Parasitology Today. 5:1989;209-213.
2. Polderman A. M., Blotkamp J. Oeso- phagostomum. Parasitology Today. 11:1995;451-456.
3. Christensen C. M., Barnes E. H., Nansen P., Roepstorff A., Slotved H.-C. ExperimentalOeso- phagostomum dentatum. International Journal for Parasitology. 25:1995;1491-1498.
4. Haupt W. Ein Beitrag zur Morphologie der Knötchenwürmer des Hausschweines, ihrer Eier sowie der dritten invasionstüchtigen Larvenstadien. Archiv für Experimentelle Veterinärmedizin. 20:1966;701-711.
5. Poelvoorde J. Oesophagostomosis in sows. Zentralblatt für Veterinärmedizin. 25:1978;835-840.
6. Poelvoorde J. Some characteristics of a laboratory produced strain ofOesophagostomum dentatum. Veterinary Parasitology. 4:1978;369-376.
7. Campbell A. J. D., Gasser R. B., Chilton N. B. Differences in a ribosomal sequence ofStrongylus. International Journal for Parasitology. 25:1995;359-365.
8. Chilton N. B., Gasser R. B., Beveridge I. Differences in a ribosomal DNA sequence of morphologically indistinguishable species within theHypodontus macropi. International Journal for Parasitology. 25:1995;647-651.
9. Gasser R. B., Hoste H. Genetic markers for closely-related nematodes. Molecular and Cellular Probes. 9:1995;315-320.
10. Gasser R. B., Stevenson L. A., Chilton N. B., Nansen P., Bucknell D. G., Beveridge I. Species markers for equine strongyles detected in intergenic spacer rDNA by PCR-RFLP. Molecular and Cellular Probes. 10:1996;371-378.
11. Hoste H., Chilton N. B., Gasser R. B., Beveridge I. Differences in the second internal transcribed spacer (ribosomal DNA) between five species ofTrichostrongylus. . International Journal for Parasitology. 25:1995;75-80.
12. Hung G. C., Jacobs D. E., Krecek R. S., Gasser R. B., Chilton N. B. Strongylus asiniStrongylus. International Journal for Parasitology. 26:1996;1407-1411.
13. Stevenson L. A., Chilton N. B., Gasser R. B. Differentiation ofHaemonchus placeiH. contortus. International Journal for Parasitology. 25:1995;483-488.
14. Gasser R. B., Chilton N. B., Hoste H., Beveridge I. Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Research. 21:1993;2525-2526.
15. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. .2nd edn. Salem: Cold Spring Harbor Laboratory Press. 1989.
16. Mullis K. B., Faloona F., Scharf S., Saiki R., Horn G., Erlich H. Specific enzymatic amplification of DNAin vitro. Cold Spring Harbor Symposium of Quantitative Biology. 51:1986;263-273.
17. Yap E. P. H., Lo, Y-M. O., Fleming K. A., McGee J. O'D. False-positives and contamination in PCR. InPCR Technology - Current Innovations. Boca Raton: CRC Press. 1994;249-258.