Purification, characterization, and in vitro phosphorylation of the neuron-specific membrane-associated protein SCG 10

Protein Expression and Purification

Volumn 9, Issue 3, 1997, Pages 363-371

  Antonsson, Bruno ^a   Lütjens, Robert ^a   Di Paolo, Gilbert ^a   Kassel, Daniel ^a,b   Allet, Bernard ^a   Bernard, Alain ^a   Catsicas, Stefan ^a   Grenningloh, Gabriele ^a  

^a GLAXOSMITHKLINE   (United States)

^b Formerly CombiChem Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ESCHERICHIA COLI;

EID: 0031127608     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0710     Document Type: Article

References (38)

1. Stein R., Mori N., Matthews K., Lo L.-C., Anderson D. J. The NGF-inducible SCG10 mRNA encodes a novel membrane-bound protein present in growth cones and abundant in developing neurons. Neuron. 1:1988;463-476.
2. Anderson D. J. Development and plasticity of a neuronal crest-derived neuroendocrine sublinage. The Assembly of the Nervous System. 1989;Alan R. Liss, New York. p. 17-36.
3. Anderson D. J., Axel R. Molecular probes for the development and plasticity of neural crest derivatives. Cell. 42:1985;649-662.
4. Himi T., Okazaki T., Wang H., McNeill T. H., Mori N. Differential localization of SCG10 and p19/stathmin messenger RNAs in adult rat brain indicates distinct roles for these growth-associated proteins. Neuroscience. 60:1994;907-926.
5. McNeill T. H., Cheng H. W., Rafols J. A., Mori N. Neuroplasticity and Parkinson's disease. Progress in Parkinson Research II. 1992;Plenum Press, New York. p. 299-323.
6. Schubart U. K., Banerjee M. D., Eng J. Homology between the cdnas encoding phosphoprotein p19 and SCG10 reveals a novel mammalian gene family preferentially expressed in developing brain. DNA. 8:1989;389-398.
7. Sobel A. Stathmin: A relay phosphoprotein for multiple signal transduction? Trends Biochem. Sci. 16:1991;301-305.
8. Sobel A., Boutterin M.-C., Beretta L., Chneiweiss H., Doye V., Peyro-Saint-Paul H. Intracellular substrates for extracellular signaling: Characterization of a ubiquitous, neuron-enriched phosphoprotein (stathmin). J. Biol. Chem. 264:1989;3765-3772.
9. Koppel J., Boutterin M. C., Doye V., Peyro-Saint-Paul H., Sobel A. Developmental tissue expression and phylogenetic conservation of stathmin, a phosphoprotein associated with cell regulations. J. Biol. Chem. 265:1990;3703-3707.
10. Belmont L. D., Mitchison T. J. Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules. Cell. 84:1996;623-631.
11. Schubart U. K. Regulation of protein phosphorylation in hamster insulinoma cells. J. Biol. Chem. 257:1982;12231-12238.
12. Feuerstein N., Cooper H. L. Rapid protein phosphorylation induced by phorbol ester in HL-60 cells. J. Biol. Chem. 258:1983;10786-10793.
13. Sobel A., Tashijian A. H. Distinct patterns of cytoplasmic protein phosphorylation related to regulation of synthesis and release of prolactin by GH cells. J. Biol. Chem. 258:1983;10312-10324.
14. Pasmantier R., Danoff A., Fleischer N., Schubart U. K. P19, a hormonally regulated phosphoprotein of peptide hormone-producing cells: Secretagogue-induced phosphorylation in att-20 mouse pituitary tumor cells and in rat and hamster insulinoma cells. Endocrinology. 119:1986;1229-1238.
15. Beretta L., Boutterin M.-C., Drouva S. V., Sobel A. Phosphorylation of a group of proteins related to physiological, multihormonal regulations of the various cell types in the anterior pituary gland. Endocrinology. 125:1989;1358-1364.
16. Cooper H. L., McDuffie E., Braverman R. Human peripheral lymphocyte growth regulation and response to phorbol esters is linked to synthesis and phosphorylation of the cytosolic protein, prosolin. J. Immunol. 143:1989;956-963.
17. Doye V., Boutterin M. C., Sobel A. Phosphorylation of stathmin and other proteins related to nerve growth factor-induced regulation of pc12 cells. J. Biol. Chem. 265:1990;11650-11655.
18. Marklund U., Brattsand G., Shingler V., Gullberg M. Serine 25 of oncoprotein 18 is a major cytosolic target for the mitogen-activated protein kinase. J. Biol. Chem. 268:1993;15039-15047.
19. cdc2. J. Biol. Chem. 268:1993;20076-20084.
20. I. A. Leighton, P. Curmi, D. G. Campbell, P. Cohen, A. Sobel, 1993, The phosphorylation of stathmin by map kinase, Mol. Cell. Biochem., 127-128, 151, 156.
21. Brattsand G., Marklund U., Nylander K., Roos G., Gullberg M. Cell-cycle-regulated phosphorylation of oncoprotein 18 on ser16, ser25 and ser38. Eur. J. Biochem. 220:1994;359-368.
22. cdc2. J. Biol. Chem. 269:1994;10312-10318.
23. Marais R. M., Parker P. J. Purification and characterisation of bovine brain protein kinase C isotypes alpha, beta and gamma. Eur. J. Biochem. 182:1989;129-137.
24. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor.
25. Allet B., Payton M., Mattaliano R., Gronenborn A. M., Clore G. M., Wingfield P. T. Purification and characterization of the DNA-binding protein Ner of bacteriophage Mu. Gene. 65:1988;259-268.
26. Bernard A. R., Radford K., Payton M. A. Current Protocols in Protein Science. 1995;Wiley, New York.
27. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
28. Provencher S. W., Gloeckner J. Estimation of globular protein secondary structure from circular dichromism. Biochemistry. 20:1981;33-37.
29. Bobba A., Cavatorta P., Attimonelli M., Riccio P., Masotti L., Quagliarello E. Estimation of protein secondary structure from circular dichromism spectra: A critical examination of the CONTIN program. Protein Seq. Data Anal. 3:1990;7-10.
30. Cohen P. The structure and regulation of protein phosphatases. Annu. Rev. Biochem. 58:1989;453-508.
31. Witters L. A. Protein phosphorylation and dephosphorylation. Curr. Opin. Cell Biol. 2:1990;212-220.
32. Curmi P. A., Maucuer A., Asselin S., Lecourtois M., Chaffotte A., Schmitter J.-M., Sobel A. Molecular characterization of human stathmin expressed inEscherichia coli: Biochem. J. 300:1994;331-338.
33. Antonsson B., Montessuit S., Di Paolo G., Lutjens R., Grenningloh G. Expression, purification and characterization of a highly soluble N-terminal truncated form of the neuron-specific membrane-associated phosphoprotein SCG10. Protein Exp. Purif. 9:1997;295-300.
34. Maucuer A., Moreau J., Mechali M., Sobel A. Stathmin gene family: Phylogenetic conservation and developmental regulation in Xenopus. J. Biol. Chem. 268:1993;16420-16429.
35. Doye V., le Gouvello S., Dobransky T., Chneiwiss H., Beretta L., Sobel A. Expression of transfected stathmin cDNA reveals novel phosphorylated forms associated with developmental and functional cell regulation. Biochem. J. 287:1992;549-554.
36. c-src. Mol. Cell. Biol. 3:1983;1157-1162.
37. Horne W. C., Neff L., Chatterjee D., Lomri A., Levy J. B., Baron R. Osteoclasts express high levels of pp60c-src in association with intracellular membranes. J. Cell Biol. 119:1992;1003-1013.
38. 2. J. Biol. Chem. 272:1997;5175-5182.