Development and use of specific polymerase reaction for the detection of an organism resembling Ehrlichia sp. in white-tailed deer

Journal of Wildlife Diseases

Volumn 33, Issue 2, 1997, Pages 246-253

  Little, Susan E ^a   Dawson, Jacqueline E ^b   Lockhart, J Mitchell ^a   Stallknecht, David E ^a   Warner, Cynthia K ^a   Davidson, William R ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

Author keywords

Ehrlichia chaffeensis; Ehrlichia sp.; Ehrlichiosis; Epizootiology; Odocoileus virginianus; Serology; White tailed deer

Indexed keywords

BACTERIAL DNA; BACTERIUM ANTIBODY; PRIMER DNA; RIBOSOME DNA; RNA 16S;

AGAR GEL ELECTROPHORESIS; ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; CHEMISTRY; DEER; EHRLICHIA; EHRLICHIOSIS; GENETICS; IMMUNOLOGY; INFESTATION; ISOLATION AND PURIFICATION; MICROBIOLOGY; PARASITE VECTOR; POLYMERASE CHAIN REACTION; PREVALENCE; TICK; UNITED STATES;

ANIMALS; ANTIBODIES, BACTERIAL; ARACHNID VECTORS; DEER; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; EHRLICHIA; EHRLICHIOSIS; ELECTROPHORESIS, AGAR GEL; POLYMERASE CHAIN REACTION; PREVALENCE; RNA, RIBOSOMAL, 16S; SOUTHEASTERN UNITED STATES; TICK INFESTATIONS; TICKS;

EID: 0031114296     PISSN: 00903558     EISSN: None     Source Type: Journal    
DOI: 10.7589/0090-3558-33.2.246     Document Type: Article

References (23)

1. ANDERSON, B. E., J. W. SUMNER, J. E. DAWSON, T. TZIANABOS, C. GREENE, J. G. OLSON, D. B. FISHBEIN, M. OLSEN-RASMUSSEN, B. P. HOLLOWAY, E. H. GEORGE, AND A. AZAD. 1992. Detection of the etiologic agent of human ehrlichiosis by PCR. Journal of Clinical Microbiology 30: 775-780.
2. _, K. SIMS, J. OLSON, J. CHILDS, J. PIESMAN, C. HAPP, G. MAUPIN, AND B. JOHNSON. 1993. Amblyomma americanum: A potential vector of human ehrlichiosis. American Journal of Tropical Medicine and Hygiene 49: 239-244.
3. BAKKEN, J. S., J. S. DUMLER, S. M. CHEN, M. R. ECKMAN, L. L. VAN ETTA, AND D. H. WALKER. 1994. Human granulocytic ehrlichiosis in the upper Midwest United States. Journal of the American Medical Association 272: 212-218.
4. CHEN, S. M., J. S. DUMLER, J. S. BAKKEN, AND D. H. WALKER. 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. Journal of Clinical Microbiology 32: 589-595.
5. DAWSON, J. E., B. E. ANDERSON, D. B. FISHBEIN, J. L. SANCHEZ, C. S. GOLDSMITH, K. H. WILSON, AND C. W. DUNTLEY. 1991a. Isolation and characterization of an Ehrlichia sp. from a patient with human ehrlichiosis. Journal of Clinical Microbiology 29: 2741-2745.
6. _, Y. RIKIHISA, S. A. EWING, AND D. B. FISHBEIN. 1991b. Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates. The Journal of Infectious Diseases 163: 564-567.
7. _, J. E. CHILDS, K. L. BIGGIE, C. MOORE, D. STALLKNECHT, J. SHADDOCK, J. BOUSEMAN, E. HOFMEISTER, AND J. G. OLSON. 1994a. White-tailed deer as a potential reservoir of Ehrlichia spp. Journal of Wildlife Diseases 30: 162-168.
8. _, D. E. STALLKNECHT, E. W. HOWERTH, C. WARNER, K. BIGGIE, W. R. DAVIDSON, J. M. LOCKHART, V. F. NETTLES, J. G. OLSON, AND J. E. CHILDS. 1994b. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal of Clinical Microbiology 32: 2725-2728.
9. _, C. K. WARNER, V. BAKER, S. A. EWING, D. E. STALLKNECHT, W. R. DAVIDSON, A. A. KOCAN, J. M. LOCKHART, AND J. G. OLSON. 1996. Ehrlichia-like 16S rRNA sequence from wild white-tailed deer (Odocoileus virginianus). The Journal of Parasitology. 82: 52-58.
10. DUMLER, J. S., K. M. ASANOVICH, J. S. BAKKEN, P. RICHTER, R. KIMSEY, AND J. E. MADIGAN. 1995. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. Journal of Clinical Microbiology 33: 1098-1103.
11. ENG, T. R., J. R. HARKESS, D. B. FISHBEIN, J. E. DAWSON, C. N. GREENE, M. A. REDUS, F. T. SATALOWICH. 1990. Epidemiologic, clinical, and laboratory findings of human ehrlichiosis in the United States, 1988. Journal of the American Medical Association 264: 2251-2258.
12. EWING, S. A., J. E. DAWSON, A. A. KOCAN, R. W. BARKER, C. K. WARNER, R. J. PANCIERA, J. C. FOX, K. M. KOCAN, AND E. F. BLOUIN. 1995. Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae). Journal of Medical Entomology 32: 368-374.
13. GOODMAN, J. L., C. NELSON, B. VITALE, J. E. MADIGAN, J. S. DUMLER, T. J. KURTH, AND U. G. MUNDERLOH. 1996. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. The New England Journal of Medicine 334: 209-215.
14. LOCKHART, J. M., W. R. DAVIDSON, J. E. DAWSON, AND D. E. STALLKNECHT. 1995. Temporal association of Amblyomma americanum with the presence of Ehrlichia chaffeensis reactive antibodies in white-tailed deer. Journal of Wildlife Diseases 31: 119-124.
15. _, W. R. DAVIDSON, D. E. STALLKNECHT, AND J. E. DAWSON. 1996. Site-specific geographic association between Amblyomma americanum (Acari: Ixodidae) infestations and Ehrlichia chaffeensis-reactive (Rickettsiales: Ehrlichieae) antibodies in white-tailed deer. Journal of Medical Entomology 33: 153-158.
16. MADIGAN, J. E., P. J. RICHTER, R. B. KIMSEY, J. E. BARLOUGH, J. S. BAKKEN, AND J. S. DUMLER. 1995. Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. The Journal of Infectious Diseases 172: 1141-1144.
17. MAGNARELLI, L. A., K. C. STANFORD, T. N. MATHER, M. YEH, K. D. HORN, AND J. S. DUMLER. 1995. Hemocytic rickettsia-like organisms in ticks: Serologic reactivity with antisera to ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR. Journal of Clinical Microbiology 33: 2710-2714.
18. NYINDO, M., I. KAKOMA, AND R. HANSEN. 1991. Antigenic analysis of four species of the genus Elirlichia by use of protein immunoblot. American Journal of Veterinary Research 52: 1225-1230.
19. OTT, R. L. 1993. An introduction to statistical methods and data analysis, 4th ed. Duxbury Press, Belmont, California, pp. 354-434.
20. PANCHOLI, P., C. P. KOLBERT, P. D. MITCHELL, K. D. REED, J. S. DUMLER, J. S. BAKKEN, S. R. TELFORD, AND D. H. PERSING. 1995. Ixodes dammini as a potential vector of human granulocytic. ehrlichiosis. The Journal of Infectious Diseases 172: 1007-1012.
21. SHANKARAPPA, B., S. K. DUTTA, AND B. L. MATTINGLY-NAPIER. 1992. Antigenic and genomic relatedness among Ehrlichia risticii, Ehrlichia sennetsu, and Ehrlichia canis. International Journal of Systematic Bacteriology 42: 127-132.
22. STRICKLAND, R. K., R. R. GERRISH, J. L. HOURRIGAN, AND G. O. SCHUBERT. 1976. Ticks of veterinary importance. Agricultural Handbook No. 485, Animal and Health Plant Inspection Service, U.S. Department of Agriculture, Washington, D.C., 122 pp.
23. _, _, AND J. S. SMITH. 1981. Arthropods. In Diseases and parasites of white-tailed deer, W. R. Davidson (ed.). Tall Timbers Research Station, Tallahassee, Florida, pp. 363-389.