Direct examination of subgingival plaque from a diseased periodontal site using confocal laser scanning microscopy (CLSM)

New Microbiologica

Volumn 20, Issue 2, 1997, Pages 155-159

  Assmus B ^a   Hausner, M ^a   Grenier, D ^b   Schloter, M ^a   Hartmann, A ^a   Goldner, M ^b  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

CLSM; Immunofluorescence; P. gingivalis; Periodontal disease; Polyclonal antiserum; Subgingival plaque

Indexed keywords

BACTERIUM ANTIBODY;

ARTICLE; BACTERIAL INFECTION; CONFOCAL MICROSCOPY; GINGIVA; HUMAN; IMMUNOLOGY; METHODOLOGY; MICROBIOLOGY; PERIODONTAL DISEASE; PORPHYROMONAS GINGIVALIS; TOOTH PLAQUE;

ANTIBODIES, BACTERIAL; BACTEROIDACEAE INFECTIONS; DENTAL PLAQUE; GINGIVA; HUMANS; MICROSCOPY, CONFOCAL; PERIODONTAL DISEASES; PORPHYROMONAS GINGIVALIS;

EID: 0031111698     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. AMANN, R.I., LUDWIG, W., and SCHLEIFER, K.-H. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews, 59, 143-169.
2. GERSDORF, H., PELZ, K., and GÖBEL, U.B. (1993). Fluorescence in situ hybridization for direct visualization of Gram-negative anaerobes in subgingival plaque samples. FEMS Immunology and Medical Microbiology, 6, 109-114.
3. GOLDNER, M., COQUIS-RONDON, M., and CARLIER, J.P. (1991). Demonstration by confocal laser scanning microscopy of invasive potential with Bacteroides fragilis. Microbiologica, 14, 71-75.
4. GOLDNER, M., COQUIS-RONDON, M., and CARLIER, J.P. (1993). Effect of growth of Bacteroides fragilis at different redox levels on potential pathogenicity in a Hela cell system: Demonstration by confocal laser scanning microscopy. Zentralblatt für Bakteriologie, 278, 529-540.
5. GONZALEZCABEZAS, C., Li, Y., NOBLITT, T.W., GREGORY, R.L., KAFRAWY, A.H., and STOOKEY, G.K. (1995). Detection of mutans streptococci in secondary carious lesions using immunofluorescent techniques and confocal laser scanning microscopy. Caries Research, 29, 198-203.
6. GORMAN, S.P., MAWHINNEY, W.M., and ADAIR, C.G. (1993). Confocal laser scanning microscopy of adherent microorganisms, biofilms and surfaces. In: Denyer, S.P., Gorman, S.P., Sussman, M. Eds, Microbial Biofilms: Formation and Control, The Society for Applied Bacteriology Technical Series No. 30. Chapter 5, pp. 95-107. Blackwell Scientific Publications, Oxford (UK).
7. HAFFAJEE, A.D., and SOCRANSKY, S.S. (1994). Microbial etiological aspects of destructive periodontal disease. Periodontology 2000, 5, 78 - 111.
8. LAMONT, R.J., CHAN, A., BELTON, C.M., IZUTSU, K.T., VASEL, D., and WEINBERG, A. (1995). Porphyromonas gingivalis invasion of gingival epithelial cells. Infection and Immunity, 63, 3878-3885.
9. MADIANOS, P.N., PAPAPANOU, P.N., NANNMARK, U., DAHLEN, G., and SANDROS, J. (1996). Porphyromonas gingivalis FDC381 multiplies and persists within human oral epithelial cells in vitro. Infection and Immunity, 64, 660-664.
10. MANZ, W., AMANN, R.I., LUDWIG, W., WAGNER, M., and SCHLEIFER, K.-H. (1992). Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Systematic and Applied Microbiology, 15, 593-600.
11. MAYRAND, D., MOUTON, C., and GRENIER, D. (1991). Chemical and biological properties of cell surface components of oral black-pigmented Bacteroides species. In: Hamadas, S., Holt, S.C., and McGhee, J.R. Eds, Periodontal disease: Pathogens and host immune responses. pp. 99-115. Quintessence Publishing Co. (Tokyo).
12. MCFETERS, G.A., YU, F.P., PYLE., B.H., and STEWART, P.S. (1995). Physiological assessment of bacteria using fluorochromes. Journal of Microbiological Methods, 21, 1-13.
13. SCHLOTER, M., BORLINGHAUS, R., BODE, W., and HARTMANN, A. (1993). Direct identification and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal laser microscopy. Journal of Microscopy, 171, 173-177.
14. SLOTS. J., and RAMS, T.E. (1993). Pathogenicity. In: Shah, H.N., Mayrand, D.D., and Genco, R.J. Eds, Biology of the species Porphyromonas gingivalis. pp. 127-138. CRC Press (Boca Raton).
15. SREENIVASAN, P.R., MEYER, D.H., and FIVES-TAYLOR, P.M. (1993). Requirements for invasion of epithelial cells by Actinobacillus actinomycetemcomitans. Infection and Immunity, 63, 3878-3885.
16. SYED, S.A., and LOESCHE, W.J. (1972). Survival of human dental plaque flora in various transparent media. Applied Microbiology, 24, 638-644.
17. WELLS, S., and JOHNSON, I. (1994). Fluorescent labels for confocal microscopy. In: Stevens, J.K. et al. (Cell Biology Ser.) Eds, Three-dimensional confocal microscopy: Volume investigation of biological specimens. Chapter 5. pp. 101-115 . Academic Press, New York (USA).
18. WILLIAMS. R.C. (1990). Periodontal disease. New England Journal of Medicine, 322, 373-382.