Variations in the relative mRNA levels of actins and myosin heavy chains do not produce corresponding differences in their proteins in the adult human heart

Journal of Molecular and Cellular Cardiology

Volumn 29, Issue 3, 1997, Pages 895-905

  Coumans, Joëlle V F ^a   Yeoh, Thomas ^a   Seeto, Reginald K ^a   Keogh, Anne ^b   Brennan, Karen ^c   Gunning, Peter ^c   Hardeman, Edna ^c   Dos Remedies, Cristobal G ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b ST VINCENT S HOSPITAL   (Australia)

^c CHILDREN'S MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

Contractile proteins; Dilated cardiomyopathy; Electrophoresis; Human heart analysis; Regulation of protein expression

Indexed keywords

ACTIN; CONTRACTILE PROTEIN; MESSENGER RNA; MYOSIN HEAVY CHAIN;

ADULT; ARTICLE; CLINICAL ARTICLE; FEMALE; HEART DILATATION; HEART FAILURE; HEART LEFT VENTRICLE; HUMAN; HUMAN TISSUE; MALE; PRIORITY JOURNAL; SARCOMERE;

EID: 0031106192     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1996.0317     Document Type: Article

References (34)

1. ALONSO S, GARNER I, VANDEKERCKHOVE J, BUCKINGHAM M, 1990. Genetic analysis of the interaction between cardiac and skeletal actin gene expression in striated muscle of the mouse. J Mol Biol 211: 727-738.
2. BEALL CJ, SEPANSKI MA, FYRBERG EA, 1989. Genetic dissection of Drosophila myofibril formation: effects of actin and myosin heavy chain null alleles. Gene Dev 3: 131-140.
3. BOHELER KR, CARRIER L, DE LA BASTIE D, ALLEN PD, KOMAJDA M, MERCADIER JJ, SCHWARTZ K, 1991. Skeletal actin mRNA increases in the human heart during ontogenic development and is the major isoform of control and failing adult hearts. J Clin Invest 88: 323-330.
4. BRADFORD MM, 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
5. BRENNAN KJ, HARDEMAN EC, 1993. Quantitative analysis of the human alpha-skeletal actin gene in transgenic mice. J Biol Chem 268: 719-725.
6. BUCHER EA, MAISONPIERRE PC, KONIECZNY SF, EMERSON CJ, 1988. Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles. Mol Cell Biol 8: 4134-4142.
7. BUGAISKY LB, ANDERSON PG, HALL RS, BISHOP SP, 1990. Differences in myosin isoform expression in the subepicardial and subendocardial myocardium during cardiac hypertrophy in the rat. Circ Res 66: 1127-1132.
8. CARVATTI M, MINTY A, ROBERT B, MONTARRAS D, WEYDERT A, COHEN A, DAUBAS P, BUCKINGHAM M, 1982. Regulation of muscle gene expression. The accumulation of messenger RNAs coding for muscle-specific proteins during myogenesis in a mouse cell line. J Mol Biol 160: 59-76.
9. CHOMCZYNSKI P, SACCHI N, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
10. CLEVELAND DW, LOPATA MA, MACDONALD RJ, COWAN NJ, RUTTER WJ, KIRSCHNER MW, 1980. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. Cell 20: 95-105.
11. DAVIES MJ, 1984. The cardiomyopathies: a review of terminology, pathology and pathogenesis. Histopathology 8: 363-393.
12. DENHARDT DT, 1966. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23: 641-646.
13. DEVLIN RB, EMERSON CJ, 1978. Coordinate regulation of contractile protein synthesis during myoblast differentiation. Cell 13: 599-611.
14. DEVLIN RB, EMERSON CJ, 1979. Coordinate accumulation of contractile protein mRNAs during myoblast differentiation. Dev Biol 69: 202-216.
15. DURAND JB, ABCHEE AB, ROBERTS R, 1995. Molecular and clinical aspects of inherited cardiomyopathies. Ann Med 27: 311-317.
16. FERRANS VJ, 1989. Pathologic anatomy of the dilated cardiomyopathies. Am J Cardiol 64: 9C-11C.
17. GARFINKEL LI, PERIASAMY M, NADAL-GINARD B, 1982. Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin. J Biol Chem 257: 11078-11086.
18. GARNER I, SASSOON D, VANDEKERCKHOVE J, ALONSO S, BUCKINGHAM ME, 1989. A development study of the abnormal expression of alpha-cardiac and alpha-skeletal actins in the striated muscle of a mutant mouse. Dev Biol 134: 236-245.
19. GUNNING P, PONTE P, BLAU H, KEDES L, 1983. Alpha-skeletal and alpha-cardiac actin genes are coexpressed in adult human skeletal muscle and heart. Mol Cell Biol 3: 1985-1995.
20. GUNNING P, PONTE P, KEDES L, HICKEY RJ, SKOULTCHI AI, 1984. Expression of human cardiac actin in mouse L cells: a sarcomeric actin associates with a nonmuscle cytoskeleton. Cell 36: 709-715.
21. KEELING PJ, GANG Y, SMITH G, SEO H, BENT SE, MURDAY V, CAFORIO AL, MCKENNA WJ, 1995. Familial dilated cardiomyopathy in the United Kingdom. Br Heart J 73: 417-421.
22. LAEMMLI UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
23. LITTEN RZ, MARTIN BJ, BUCHTHAL RH, NAGAI R, Low RB, ALPERT NR, 1985. Heterogeneity of myosin isozyme content of rabbit heart. Circ Res 57: 406-414.
24. MAYER NJ, RUBIN SA, 1995. The molecular and cellular biology of heart failure. Curr Opin Cardiol 10: 238-245.
25. MEDFORD RM, NGUYEN HT, NADAL-GINARD B, 1983. Transcriptional and cell cycle-mediated regulation of myosin heavy chain gene expression during muscle cell differentiation. J Biol Chem 258: 11063-11073.
26. MESTRONI L, KRAIJINOVIC M, SEVERINI GM, FALASCHI A, GIACCA M, CAMERINI F, 1994. Molecular genetics of dilated cardiomyopathy. Herz 19: 97-104.
27. PAGANI ED, ALOUSI AA, GRANT AM, OLDER TM, DZIUBAN SW, ALLEN PD, 1988. Changes in myofibrillar content and Mg-ATPase activity in ventricular tissues from patients with heart failure caused by coronary artery disease, cardiomyopathy, or mitral valve insufficency. Circ Res 63: 380-385.
28. PALMITER RD, 1974. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysemes and messenger ribonucleic acid. Biochemistry 13: 3606-3615.
29. SAMBROOK J, FRITSCH EF, MANIATIS T, 1989. Molecular cloning: A laboratory Manual. New York, Cold Spring Harbor Laboratory Press.
30. SHANI M, ZEVIN SD, SAXEL O, CARMON Y, KATCOFF D, NUDEL U, YAFFE D, 1981. The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis. Dev Biol 86: 483-492.
31. SOMERVILLE LL, WANG K, 1987. In vivo phosphorylation of titin and nebulin in frog skeletal muscle. Biochem Biophys Res Commun 147: 986-992.
32. SWYNGHEDAUW B, 1986. Developmental and functional adaptation of contractile proteins in cardiac and skeletal muscles. Physiol Rev 66: 710-771.
33. TRAHAIR T, YEOH T, CARTMILL T, KEOGH A, SPRATT P, CHANG V, DOS REMEDIOS CG, GUNNING P, 1993. Myosin light chain gene expression associated with disease states of the human heart. J Mol Cell Cardiol 25: 577-585.
34. WADE R, SUTHERLAND C, GAHLMANN R, KEDES L, HARDEMAN E, GUNNING P, 1990. Regulation of contractile protein gene family mRNA pool sizes during myogenesis. Dev Biol 142: 270-282.