Formation of intracellular free radicals in guinea pig airway epithelium during in vitro exposure to ozone

Toxicology and Applied Pharmacology

Volumn 143, Issue 1, 1997, Pages 96-101

  Chen, Lung Chi ^a   Qu, Qingshan ^a  

^a NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIETHYLDITHIOCARBAMIC ACID; DIMETHYL SULFOXIDE; FORMIC ACID; FREE RADICAL; OZONE; REACTIVE OXYGEN METABOLITE; SODIUM AZIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOCHEMISTRY; DOSE RESPONSE; DRUG COMPARISON; EPITHELIUM CELL; FLUORESCENCE; GUINEA PIG; IN VITRO STUDY; MALE; NONHUMAN; RESPIRATORY EPITHELIUM;

ANIMALIA; CAVIA PORCELLUS; LETHRINIDAE; SUS SCROFA;

EID: 0031105999     PISSN: 0041008X     EISSN: None     Source Type: Journal    
DOI: 10.1006/taap.1996.8073     Document Type: Article

References (30)

1. Adler, K. B., Schwarz, J. E., Whitcutt, M. J., and Wu, R. (1987). A new chamber system for maintaining differentiated guinea pig respiratory epithelial cells between air and liquid phase. BioTechniques 5, 462-465.
2. Adler, K. B., Cheng, P. W., and Kim, K. (1990). Characterization of guinea pig tracheal epithelial cells maintained in biphasic organotypic culture: Cellular composition and biochemical analysis of released glycoconjugates. Am. J. Respir. Cell Mol. Biol. 2, 145-154.
3. Cheek, J. M., Buckpitt, A. R., Li, C., Tarkington, B. K., and Plopper, C. G. (1994). Ozone injury to alveolar epithelium in vitro does not reflect loss of antioxidant defenses. Toxicol. Appl. Pharmacol. 125(1), 59-69.
4. Chen, L. C., Fang, C. P., Qu, Q. S., Fine, J. M., and Schlesinger, R. B. (1993). A novel system for the in vitro exposure of pulmonary cells to acid sulfate aerosols. Fundam. Appl. Toxicol. 20, 170-176.
5. Cohen, D. S., Palmer, E., Welch, W. J., and Sheppard, D. (1991). The response of guinea pig airway epithelial cells and alveolar macrophages to environmental stress. Am. J. Respir. Cell Mol. Biol. 5(2), 133-143.
6. Cueto, R., Squadrito, G. L., Bermudez, E., and Pryor, W. A. (1992). Identification of heptanal and nonanal in bronchoalveolar lavage from rats exposed to low levels of ozone. Biochem. Biophys. Res. Commun. 188, 129-134.
7. Devlin, R. B., McKinnon, K. P., Noah, T., Becker, S., and Koren, H. S. (1994). Ozone-induced release of cytokines and fibronectin by alveolar macrophages and airway epithelial cells. Am. J. Physiol. 266(6), L612-619.
8. Driscoll, K. E., and Schlesinger, R. B. (1988). Alveolar macrophage-stimulated neutrophil and monocyte migration: Effects of in vitro ozone exposure. Toxicol. Appl. Pharmacol. 93, 312-318.
9. Emmendorffer, A., Hecht, M., Lohmann-Matthes, M. L., and Roesler, J. (1990). A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123. J. Immunol. Methods 131, 269-275.
10. Grose, E. C., Gardner, D. E., and Miller, F. J. (1980). Response of ciliary epithelium to ozone and sulfuric acid. Environ. Res. 22, 377-385.
11. Harkema, J. R., Plopper, C. G., Hyde, D. M., St George, J. A., Wilson, D. W., and Dungworth, D. L. (1987). Response of the Macaque nasal epithelium to ambient levels of ozone. A morphologic and morphometric study of the transitional and respiratory epithelium. Am. J. Pathol. 128(1), 29-44.
12. Last, J. A., Jennings, M. D., Schwartz, L. W., and Cross, C. E. (1977). Glycoprotein secretion by tracheal explants cultured from rats exposed to ozone. Am. Rev. Respir. Dis. 116, 695-703.
13. Leikauf, G. D., Zhao, Q., Zhou, S., and Santrock, J. (1993). Ozonolysis products of membrane fatty acids activate eicosanoid metabolism in human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 9, 594-602.
14. Lippmann, M. (1989). Health effects of ozone, a critical review. AWMA 39, 672-685.
15. Mustafa, M. G. (1990). Biochemical basis of ozone toxicity. Free Radical Biol. Med. 9, 245-265.
16. Nikula, K. J., Wilson, D. W., Giri, S. M., Plopper, C. G., and Dungworth, D. L. (1988). The response of the rat tracheal epithelium to ozone exposure: Injury, adaptation, and repair. Am. J. Pathol. 131(2), 373-384.
17. Pryor, W. A., and Church, D. F. (1991). Aldehydes, hydrogen peroxide, and organic radicals as mediators of ozone toxicity. Free Radical Biol. Med. 11, 41-46.
18. Pryor, W. A. (1992). How far does ozone penetrate into the pulmonary air/ tissue boundary before it reacts? Free Radical. Biol. Med. 12, 93.
19. Pryor, W. A. (1994). Mechanisms of radical formation from reactions of ozone with target molecules in the lung. Free Radical Biol. Med. 17(5), 451-465.
20. Pryor, W. A., Wang, K., and Bermudez, E. (1992). Cholesterol ozonation products as biomarkers for ozone exposure in rats. Biochem. Biophys. Res. Commun. 188, 618-623.
21. Rojanasakul, Y., Wang, L., Malanga, C. J., Ma, J. Y. C., Banks, D. E., and Ma, J. K. H. (1993). Altered calcium homeostasis and cell injury in silica-exposed alveolar macrophages. J. Cell. Physiol. 154, 310-316.
22. 2 in cultured endothelial cells. Arch. Biochem. Biophys. 302, 348-355.
23. Santrock, J., Gorski, R. A., and O'Gara, J. F. (1992). Products and mechanisms of the reaction of ozone with phospholipids in unilamella phospholipid vesicles. Chem. Res. Toxicol. 5, 134-141.
24. Stephens, R. J., Sloan, M. F., Evans, M. J., et al. (1974a). Alveolar type I cell response to exposure to ozone for short period. Exp. Mol. Pathol. 20, 11-23.
25. Stephens, R. J., Sloan, M. F., Evans, M. J., et al. (1974b). Early response of lung to low levels of ozone. Am. J. Pathol. 74, 31-58.
26. Tepper, J. S., Costa, D. L., Lehmann, J. R., Weber, M. F., and Hatch, G. E. (1989). Unattended structural and biochemical alterations in the rat lung during functional adaptation to ozone. Am. Rev. Respir. Dis. 140, 490-501.
27. USEPA (1989). OAQPS Stuff Paper: Review of the national ambient air quality standard for ozone - Assessment of scientific and technical information. US EPA June 1.
28. Valentine, R. (1985). An in vitro system for exposure of lung cells to gases: Effects of ozone on rat macrophages. J. Toxicol. Environ. Health 16, 115-126.
29. Whitcutt, M. J., Adler, K. B., and Wu, R. (1988). A biphasic chamber system for maintaining polarity of differentiation of cultured respiratory tract epithelial cells. In Vitro 24, 420-428.
30. Wilson, D. W., Plopper, C. G., and Dungworth, D. L. (1984). The response of the macqque tracheobronchial epithelium to acute ozone injury. A quantitative ultrastructural and autoradiographic study. Am. J. Pathol. 116, 193-206.