Multiple transcription start sites of the carrot dihydrofolate reductase-thymidylate synthase gene, and sub-cellular localization of the bifunctional protein

Plant Molecular Biology

Volumn 33, Issue 4, 1997, Pages 709-722

  Luo, Meizhong ^a,c   Orsi, Roberta ^a   Patrucco, Emanuela ^a   Pancaldi, Simonetta ^b   Cella, Rino ^b  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF FERRARA   (Italy)

^c CLEMSON UNIVERSITY   (United States)

Author keywords

Daucus carota; dihydrofolate reductase thymidylate synthase; immunogold labelling; microsatellite; plastid localisation; rapid amplification of 5' cDNA end (RACE)

Indexed keywords

DIHYDROFOLATE REDUCTASE; MESSENGER RNA; MULTIENZYME COMPLEX; PLANT RNA; PROTEIN PRECURSOR; THYMIDYLATE SYNTHASE; THYMIDYLATE SYNTHASE DIHYDROFOLATE REDUCTASE; THYMIDYLATE SYNTHASE-DIHYDROFOLATE REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; CARROT; CELL COMPARTMENTALIZATION; COMPARATIVE STUDY; DINUCLEOTIDE REPEAT; ENZYMOLOGY; GENE; GENETIC POLYMORPHISM; GENETIC TRANSCRIPTION; GENETICS; IMMUNOELECTRON MICROSCOPY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASTID; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL COMPARTMENTATION; DAUCUS CAROTA; DINUCLEOTIDE REPEATS; GENES, PLANT; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PLASTIDS; POLYMORPHISM, GENETIC; PROTEIN PRECURSORS; RNA, MESSENGER; RNA, PLANT; SEQUENCE ANALYSIS; TETRAHYDROFOLATE DEHYDROGENASE; THYMIDYLATE SYNTHASE; TRANSCRIPTION, GENETIC;

DAUCUS CAROTA;

EID: 0031105834     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005798207693     Document Type: Article

References (48)

1. Albani D, Parisi B, Carbonera D, Cella R: Dihydrofolate reductase from Daucus carota cell suspension cultures: purification, molecular and kinetic characterization. Plant Mol Biol 5: 363-372 (1985).
2. Altman LG, Schneider BG, Papermaster DS: Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy. J Histochem Cytochem 32: 1217-1223 (1984).
3. Appling DR: Compartmentation of folate-mediated one-carbon metabolism in eukaryotes. FASEB J 5: 2645-2651 (1991).
4. Braghetti A, Piazzi G, Lanfranco L, Mondello C: Multiple DNA-protein interactions at the CpG island of the human pseudoautosomal gene MIC 2. Somat Cell Mol Genet 19(1): 51-63 (1993).
5. Caelles C, Ferrer A, Balcells L, Hegardt FG, Boronat A: Isolation and structural characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl coenzyme A reductase. Plant Mol Biol 13: 627-638 (1989).
6. Cella R, Albani D, Carbonera D, Etteri L, Maestri E, Parisi B: Selection of methotrexate-resistant cell lines in Daucus carota: biochemical analysis and genetic characterization by protoplast fusion. J Plant Physiol 127: 135-146 (1987).
7. Cella R, Balestrazzi A, Carbonera D, Nano R, Rezzani R: Cellular localisation of dihydrofolate reductase-thymidylate synthase in carrot cells. Eur J Bas Appl Histochem 35: 61-71 (1991).
8. Cella R, Carbonera D, Orsi R, Ferri G, Iadarola: Proteolytic and partial sequencing studies of the bifunctional dihydrofolate reductase-thymidylate synthase from Daucus carota. Plant Mol Biol 16: 975-982 (1991).
9. Cella R, Parisi B: Dihydrofolate reductase and thymidylate synthase in plants: an open problem. Physiol Plant 88: 509-521 (1993).
10. Cheng S-H, Cline K, DeLisle AJ: An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5′ ends. Plant Physiol 106: 303-311 (1994).
11. Chibbar RN, van Huystee R: Immunochemical localisation of peroxidase in cultured peanut cells. J Plant Physiol 123: 477-486 (1986).
12. Cossins EA: One carbon metabolism. In: Davies DD (ed) The Biochemistry of Plants, Vol. 2, pp. 365-418. Academic Press, New York, ISBN 0-12-675402-0, (1980).
13. Cossins EA: Folate biochemistry and the metabolism of one-carbon units. In: Davies DD (ed) The Biochemistry of Plants, Vol. 11, pp. 317-353. Academic Press, San Diego, CA ISBN 0-12-675411-X (1987).
14. Craig S, Moore PJ, Dunahay TG: Immunogold localisation of intra- and extra-cellular protein and polysaccharides of plant cells. Scanning Microscopy 1: 1431-1437 (1987).
15. Della Porta SL, Woo JE, Hicks JB: A plant DNA minipreparation: version II. Plant Mol Biol Rep 2: 1-19 (1983).
16. Frohman MA: Amplifications: a forum for PCR users, no. 5, pp. 11-15. Perkin Elmer Cetus Corp. (1990).
17. Frohman MA, Dush MK, Martin GR: Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998-9002 (1988).
18. Groneborn AM, Clore GM: Characterization of the DNA binding region recognized by dihydrofolate reductase from Lactobacillus casei. J Biol Chem 258: 11256-11259 (1983).
19. Hearne CM, Ghosh S, Todd JA: Microsatellites for linkage analysis of genetic traits. Trends Genet 8: 288-294 (1992).
20. Gavel I, von Heijne G: A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett 261: 455-458 (1990).
21. Horisberger M: Colloidal gold: a cytochemical marker for light and fluorescent microscopy and for transmission and scanning microscopy. Scann Electr Micr 2: 9-31 (1981).
22. Ivanetich KM, Santi DV: Bifunctional thymidylate synthase-dihydrofolate reductase in protozoa. FASEB J 4: 1591-1597 (1990).
23. Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643-6653 (1987).
24. Kapler GM, Zhang K, Beverley SM: Sequence and S1 nuclease mapping of the 5′ region of the dihydrofolate reductase-thymidylate synthase gene of Leishmania major. Nucl Acids Res 15: 3369-3383 (1987).
25. Karnovsky MJ: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27: 137-138 (1965).
26. Lazar G, Zhang H, Goodman HM: The origin of the bifunctional dihydrofolate reductase-thymidylate synthase isogenes of Arabidopsis thaliana. Plant J 3: 657-668 (1993).
27. Luo M-Z, Cella R: A reliable amplification technique with single-sided specificity for the isolation of 5′ gene-regulating regions. Gene 140: 59-62 (1994).
28. Luo M-Z and Cella R: Analysis of the structure of the 5′ end of the gene coding for carrot dihydrofolate reductase-thymidylate synthase. In: Terzi M, Cella R, Falavigna A (eds) Current Issues in Plant Molecular and Cellular Biology, pp. 583-588. Kluwer Academic Publishers, Dordrecht (1995).
29. Luo M-Z, Piffanelli P, Rastelli L, Cella R: Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota. Plant Mol Biol 22: 427-435 (1993).
30. Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6(1): 43-48 (1987).
31. Marchuk D, Drumm M, Saulino A, Collins FS: Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucl Acids Res 19: 1154 (1991).
32. Mitchell PJ, Carothers AM, Han JH, Harding JD, Kas E, Venolia L, Chasin LA: Multiple transcription start sites, DNase I-hypersensitive sites, and an opposite-strand exon in the 5′ region of the CHO dhfr gene. Mol Cell Biol 6: 425-440 (1986).
33. Morgante M, Olivieri AM: PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175-182 (1993).
34. Noguchi H, Reddy GPV, Pardee AB: Rapid incorporation of label from ribonucleotides diphosphates into DNA by cell-free high molecular weight fraction from animal cell nuclei. Cell 32: 443-451 (1983).
35. Norris SR, Meyer SE, Callis J: The intron of Arabidopsis thaliana polyubiquitin genes in conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol Biol 21: 895-906 (1993).
36. Ogden RC, Adams DA: Guide to molecular cloning. Meth Enzymol 152: 61-87 (1987).
37. Reddy GPV, Pardee AB: Multienzyme complex for metabolic channeling in mammalian DNA replication. Proc Natl Acad Sci USA 77: 3312-3316 (1980).
38. Reichard P: Interaction between deoxyribonucleotide and DNA synthesis. Annu Rev Biochem 57: 349-374 (1988).
39. Roth J, Bendayan M, Carleman E, Villinger W, Garavito M: Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 29: 663-671 (1981).
40. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
41. Schuster DM, Buchman JW, Rasthchian A: A simple and efficient method for amplification of cDNA ends using 5′ RACE. Focus 14(2): 46-52 (1992).
42. Stein R, Sciary-Galili N, Razin A, Cedar H: Pattern of methylation of two genes coding for housekeeping functions. Proc Natl Acad Sci USA 80: 2422-2426 (1983).
43. Taylor GR, Lagosky PA, Storms RK, Haynes RH: Molecular characterization of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae. J Biol Chem 262: 5298-5307 (1987).
44. Timmers ACJ, Kieft H, Schel JHN: An immunofluorescence study on calmodulin during somatic and zygotic embryogenesis of carrot (Daucus carota L.). Acta Bot Neerl 44: 19-32 (1995).
45. Toth I, Lazar G, Goodman HM: Purification and immunochemical characterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild carrot cells. EMBO J 6: 1853-1858 (1987).
46. von Heijne G, Steppuhn J, Herrmann R: Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535-545 (1989).
47. von Heijne G, Hira T, Klogsen R-B, Steppuhn J, Brace B, Keegstra K, Herrmann R: CHLPEP-A database of chloroplast transit peptides. Plant Mol Biol Rep 9: 104-126 (1991).
48. Zelikson R, Luzzatti M: Mithocondrial and cytoplasmic distribution in Saccharomyces cerevisiae of enzymes involved in folate-coenzyme-mediated one-carbon-group transfer. Eur J Biochem 79: 285-292 (1977).