Expression of the Arabidopsis G-protein GPα1: Purification and characterisation of the recombinant protein

Plant Molecular Biology

Volumn 33, Issue 4, 1997, Pages 723-728

  Wise, Alan ^c   Thomas, Paul G ^b   Carr, T Hedley ^a   Murphy, Gillian A ^a   Millner, Paul A ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b JEALOTT S HILL RESEARCH STATION   (United Kingdom)

^c UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

affinity chromatography; dnaY; G protein; GP 1; GTP S binding; metal ions

Indexed keywords

ARGININE TRANSFER RNA; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARABIDOPSIS; ARTICLE; ESCHERICHIA COLI; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM;

ARABIDOPSIS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); PLANT PROTEINS; RECOMBINANT PROTEINS; RNA, TRANSFER, ARG;

ARABIDOPSIS; ESCHERICHIA COLI;

EID: 0031105805     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005732423622     Document Type: Article

References (31)

1. Bourne HR., Sanders DA, McCormick F: The GTPase super-family: a conserved switch for diverse cell functions. Nature 348: 125-132 (1990).
2. Strosberg AD: Structure/function relationship of proteins belonging to the family of receptors coupled to GTP-binding proteins. Eur J Biochem 196: 1-10 (1991).
3. Conklin BR, Bourne HR: Structural elements of Gα subunits that interact with Gβγ, receptors and effectors. Cell 73: 631-641 (1993).
4. Freissmuth M, Casey PJ, Gilman AG: G proteins control diverse pathways of transmembrane signalling. FASEB J 3: 2125-2131 (1989).
5. Birnbaumer L, Abramowitz J, Brown AM: Receptor-effector coupling by G-proteins. Biochim Biophys Acta 1031: 163-224 (1990).
6. White IR, Wise A, Finan PM, Clarkson J, Millner PA: GTP-binding proteins in higher plant cells. In: Cooke DT, Clarkson DT (eds) Transport and Receptor Proteins of Plant Membranes, pp. 185-192. Plenum Press, New York (1992).
7. Millner PA: Molecular characteristics and cellular roles of guanine nucleotide binding proteins in plants. In: Hall MA, Libbenga K (eds) New Comprehensive Biochemistry: Biochemistry and Molecular Biology of Plant Hormones. Elsevier, Amsterdam, in press (1994).
8. Ma H, Yanofsky MF, Meyerowitz EM: Molecular cloning and characterization of GPA1, a G protein α-subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci USA 87: 3821-3825 (1990).
9. Ma H, Yanofsky MF, Huang H: Isolation and sequence analysis of TGA1 cDNA encoding a tomato G protein α-subunit. Gene 107: 189-195 (1991).
10. Ishikawa A, Tsubouchi H, Iwasaki Y, Asahi T: Molecular cloning and characterization of a cDNA for the a subunit of a G protein from rice. Plant Cell Physiol 36: 353-359 (1995).
11. Weiss CA, Garnaat CW, Mukai K, Hu Y, Ma H: Isolation of cDNAs encoding guanine nucleotide-binding protein β-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1). Proc Natl Acad Sci USA 91: 9554-9558 (1994).
12. Huang H, Weiss CA, Ma H: Regulated expression of the Arabidopsis G-protein α-subunit gene GPA1. Int J Plant Sci 155: 3-14 (1994).
13. + channel current in guard cells of fava bean. Plant Cell 3: 1037-1044 (1991).
14. Neuhaus G, Bowler C, Kern R, Chua N-H: Calcium/ calmodulin-dependent and -independent phytochrome signal transduction pathways. Cell 73: 937-952 (1993).
15. Deng XW, Matsui M, Wei N, Wagner D, Chu AM, Feldman KA, Quail PH: COP1, an Ambidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain. Cell 71: 791-801 (1992).
16. Garcia GM, Mar PK, Mullin DA, Walker JR, Prather NE: The E. coli dnaY gene encodes an arginine transfer RNA. Cell 45: 453-459 (1986).
17. Pfeuffer E, Pfeuffer T: Preparation and application of GTP-agarose matrices. Meth Enzymol 195: 171-177 (1991).
18. Pantoliano MW, Holmquist B, Riordan JF: Affinity Chromatographic purification of angitensin converting enzyme. Biochemistry 23: 1037-1042 (1984).
19. Cuatrecasas P, Parikh I: Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose. Biochemistry 11: 2291-2299 (1972).
20. Finan PM, White IR, Redpath SH, Findlay JBC, Millner PA: Molecular cloning, sequence determination and heterologous expression of nucleoside diphosphate kinase from Pisum sativum. Plant Mol Biol 25: 59-67 (1994).
21. Tabor S, Richardson CC: A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci US 82: 1074-1078 (1985).
22. Studier FW, Moffatt BA: Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189: 113-130 (1986).
23. Northup JK, Smigel MD, Gilman AG: The guanine nucleotide activating site of the regulatory component of adenylate cyclase. J Biol Chem 257: 11416-11423 (1982).
24. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85 (1985).
25. Hill HD, Straka JG: Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents. Anal Biochem 170: 203-208 (1988).
26. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 (1970).
27. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354 (1979).
28. White IR, Zamri I, Wise A, Millner PA: Use of synthetic peptides to study G-proteins and protein kinases within plant cells. SEB Semin Ser 53: 91-108 (1993).
29. Brinkmann U, Mattes RE, Buckel P: High level expression of recombinant genes in E. coli is dependent on the availability of the dnaY gene product. Gene 85: 109-114 (1989).
30. Wise A, Thomas PG, White IR, Millner PA: Isolation of a putative receptor from Zea mays microsomal membranes that interacts with the G-protein GPα1. FEBS Lett 356: 233-237 (1994).
31. Goody RS, Fresh M, Wittinghofer A: Affinity of guaninenucleotide binding-proteins for their ligands: facts and artifacts. Trends Biochem Sci 16: 327-328 (1991).