Biocatalysis of chlorophyllase in ternary micellar systems using pheophytins as substrates

Journal of Chemical Technology and Biotechnology

Volumn 68, Issue 3, 1997, Pages 315-323

  Samaha, Hiba ^a   Kermasha, Selim ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Biocatalysis; Chlorophyllase; Micellar systems; Pheophytins; Ternary

Indexed keywords

ALGAE; AROMATIC COMPOUNDS; ENZYMES; HYDROCHLORIC ACID; MICELLES; ORGANIC SOLVENTS; SURFACE ACTIVE AGENTS;

BIOCATALYSIS; CHLOROPHYLLASE; HEXANE; PHEOPHYTINS; TERNARY MICELLAR SYSTEMS;

BIOCATALYSTS;

HEXANE; HYDROLASE; MEMBRANE ENZYME; MEMBRANE LIPID; PHEOPHYTIN; POLYSORBATE; POLYSORBATE 20; POLYSORBATE 40; POLYSORBATE 60; POLYSORBATE 80; SORBITAN; SORBITAN TRIOLEATE; SURFACTANT; TROMETAMOL;

ALGA; ARTICLE; CATALYSIS; COMPETITIVE INHIBITION; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INCUBATION TIME; PH; SPECTROPHOTOMETRY; TEMPERATURE;

EID: 0031105317     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4660(199703)68:3<315::AID-JCTB654>3.0.CO;2-9     Document Type: Article

References (31)

1. Bacon, M. F. & Holden, M., Chlorophyllase of sugar-beet leaves. Phytochem., 9 (1970) 115-25.
2. Holden, M., Analytical methods-chlorophylls. In Chemistry and Biochemistry of Plant Pigments, Vol. 2, 2nd edn, ed T. W. Goodwin. Academic Press, New York, 1976, pp. 1-37.
3. Ellsworth, R. K., Studies on chlorophyllase. I. Hydrolytic and esterification activities of chlorophyllase from wheat seedlings. Photosynthetica, 5 (1971) 226-32.
4. Martinek, K., Levashov, A. V., Klyachko, N., Khmelnitski, Y. L. & Berezin, I. V., Micellar enzymology (Review). Eur. J. Biochem., 155 (1986) 453-68.
5. Ismail, A. A., Wafik Gouda, M. & Motawi, M. M., Micellar solubilization of barbiturates I: Solubilization of certain barbiturates in polysorbates of varying hydrocarbon chain length. J. Pharm. Sci., 59 (1970) 220-4.
6. Attwood, D. & Florence, A. T., Aspects of surfactant toxicity. In Surfactant Systems, eds D. Atwood & A. T. Florence. Chapman and Hall Ltd., New York, 1983, pp. 614-97.
7. Myers, D., In Surfactant Science and Technology. VCH Publishers, New York, 1988, pp. 153-82.
8. Khamessan, A., Kermasha, S. & Mollimard, L., Optimization of chlorophyllase-catalyzed hydrolytic activity in a micellar ternary system. Biotechnol. Appl. Biochem., 22 (1995) 327-43.
9. Khamessan, A. & Kermasha, S., Biocatalysis of chlorophyllase in micellar ternary system containing Spans. J. Biotechnol., 45 (1996) 253-64.
10. Khamessan, A., Kermasha, S., Marsot, P. & Ismail, A. A., Biocatalysis of chlorophyllase from Phaeodactylum tricornutum in biphasic organic system. J. Chem. Tech. Biotechnol., 60 (1994) 73-81.
11. Khamessan, A., Kermasha, S. & Marsot, P., Effect of polar organic solvent on the biocatalysis of chlorophyllase in biphasic organic system. Biosci. Biotech. Biochem., 58 (1994) 1947-52.
12. Kuroki, M., Shioi, Y. & Sesa, T., Purification and properties of soluble chlorophyllase from tea leaf sprouts. Plant Cell Physiol., 22 (1981) 717-25.
13. Lambers, J. W. J. & Terpstra, W., Inactivation of chlorophyllase by negatively charged plant membrane lipids. Biochim. Biophys. Acta, 831 (1985) 225-35.
14. Sandermann, H., Regulation of membrane enzyme by lipids. Biochim. Biophys. Acta, 515 (1978) 209-37.
15. 2+. Biochim. Biophys. Acta, 746 (1983) 23-31.
16. Levadoux, W. L., Kalmokoff, M. L., Pickard, M. D. & Groofwassink, J. W. D., Pigment removal from canola oil using chlorophyllase. J. Am. Oil Chem. Soc., 64 (1987) 139-44.
17. Daun, J. K. & Thorsteinson, C. T., Determination of chlorophyll pigments in crude and degummed canola oil by hplc and spectrophotometry. J. Am. Oil Chem. Soc., 66 (1989) 1124-8.
18. Endo, Y., Thorsteinson, C. T. & Daun, J. K., Characterization of chlorophyll pigments present in canola seed, meal and oil. J. Am. Oil Chem. Soc., 69 (1992) 564-8.
19. Kermasha, S., Khalyfa, A., Marsot, P., Alli, I. & Fournier, R., Biomass production, purification and characterization of chlorophyllase from alga (Phaeodactylum tricornutum). Biotechnol. Appl. Biochem., 14 (1992) 142-59.
20. Hartree, E. F., Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal. Biochem., 48 (1972) 422-7.
21. Khalyfa, A., Kermasha, S. Alli, I., Extraction, purification and characterization of chlorophylls from spinach leaves. J. Agric. Food Chem., 40 (1992) 215-20.
22. Fraser, M. S. & Frankl, G., Detection of chlorophyll derivatives in soybean oil by hplc. J. Am. Oil Chem. Soc., 62 (1985) 113-21.
23. Canjura, F. L. & Schwartz, S. J., Separation of chlorophyll compounds and their polar derivatives by high-performance liquid chromatography. J. Agric. Food Chem., 39 (1991) 1102-5.
24. Overbeek, J. T. G., De Bruyen, P. L. & Verhoeckx, F., Microemulsion. In Surfactant, ed. T. F. Tadros. Academic Press, London, 1984, pp. 111-32.
25. Ottewill, R. H., Introduction. In Surfactant, ed. T. F. Tadros. Academic Press, London, 1984, pp. 1-18.
26. O'Connor, C. J., Interfacial catalysis by microphases in apolar media. In Interfacial Phenomena in Apolar Media, eds H. Eicke & G. D. Parfitt. Marcel Dekker, Inc., New York, 1987, pp. 187-255.
27. Terpstra, W., A study of properties and activity of chlorophyllase in photosynthetic membranes. Z. Planzenphysiol., 85 (1977) 139-46.
28. Khamessan, A., Kermasha, S. & Marsot, P., Biocatalysis of chlorophyllase from Phaeodactylum tricornutum in organic solvent media. Process Biochem., 30 (1994) 159-68.
29. Khamessan, A., Kermasha, S. & Marsot, P., Biocatalysis of chlorophyllase from algae Phaeodactylum tricornutum in water/miscible-organic-solvent system. Biotechnol. Appl. Biochem., 18 (1993) 285-98.
30. Daood, H. G., Czinkotai, B., Hoschke, A. & Biacs, P., High-performance liquid chromatography of chlorophylls and carotenoids from vegetables. J. Chromatogr., 472 (1989) 296-302.
31. Strain, H. H., Cope, B. T. & Svec, W. A., Analytical procedures for the isolation, identification, estimation and investigation of the chlorophylls. Methods Enzymol., 23 (1971) 452-76.