An explanation and the clinical significance of the failure of microcolumn tests to detect weak ABO and other antibodies

Transfusion Medicine

Volumn 7, Issue 1, 1997, Pages 47-53

  Phillips, P ^a   Voak, D ^b   Knowles, S ^c   Campbell, G ^b   Crane, B ^c   Downie, D M ^b   Haigh, T ^d   Whitton, C ^a  

^a NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

^b East Anglian Blood Centre   (United Kingdom)

^c South Thames Blood Centre   (United Kingdom)

^d Royal Infirmary   (United Kingdom)

Author keywords

clinical significance; DiaMed BioVue; microcolumn antiglobulin technology; missed ABO and K incompatibilities

Indexed keywords

EID: 0031104754     PISSN: 09587578     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3148.1997.d01-79.x     Document Type: Article

References (20)

1. BCSH. (1991) Standard Haematology Practices (ed. Roberts, B.). Blackwell Scientific Publications, Oxford, UK.
2. Cummins, D. & Downham, B. (1994) Failure of DiaMed ID microtyping system to detect major ABO incompatibility. Lancet, 343, 1649.
3. Economidou, J., Hughes-Jones, N.C. & Gardner, B. (1967) Quantitative measurements concerning A and B antigenic sites. Vox Sanguinis, 12, 321-328.
4. Greenbury, C.L., Moore, D.H. & Nunn, L.A.C. (1963) Reaction of 7S and 19S components of immune rabbit antisera with human group A and AB red cells. Immunology, 6, 412-433.
5. Judd, W.L. (1991) Are there better ways than the crossmatch to demonstrate ABO incompatibility? (Editorial). Transfusion, 31, 192-194.
6. Merry, A.H., Thomson, E.E., Lagar, J., Howell, P., Voak, D., Downie, D. & Stratton, F. (1984) Quantitation of antibody binding to erythrocytes in LISS. Vox Sanguinis, 47, 125-132.
7. Mollison, P.L., Engelfriet, C.P. & Contreras, M. (1987) Blood Transfusion in Clinical Medicine, 8th edn. Blackwell Scientific Publications, Oxford.
8. Molthan, L. & Strohm, P. (1981) Hemolytic transfusion reaction due to anti-Kell undetectable by low-ionic-strength solutions. American Journal of Clinical Pathology, 75, 629-631.
9. Park, H. (1985) Limitations of the immediate spin test. (Letter). Transfusion, 25, 588.
10. Phillips, P.K., Whitton, C.M. & Lavin, F. (1992) The use of the antiglobulin 'gel-test' for antibody detection. Tranfusion Medicine, 2, 111-113.
11. Pinkerton, P.H., Chan, R., Ward, J. & Coovadia, A.S. (1993a) Sensitivity of column agglutination technology in detecting unexpected red cell antibodies. Tranfusion Medicine, 3, 201-205.
12. Pinkerton, P.H., Ward, J., Chan, R. & Coovadia, A.S. (1993b) An evaluation of a gel technique for antibody screening compared with a conventional tube method. Transfusion Medicine, 3, 275-279.
13. Poole, G.D., Evans, R.G., Voak, D., Scott, M.L., Chapman, J. & Phillips, P.K. (1996) Evaluation of six systems for the detection of red cell antibodies. Medical Device Agency, Department of Health, Elephant and Castle, London.
14. Steane, E.A., Steane, S.M. Montgomery, S.R. & Pearson, J.R. (1985) A proposal for compatibility testing incorporating the manual hexadimethrine bromide (Polybrene) test. Transfusion, 25, 540-544.
15. Voak, D. (1972) Observations on the rare phenomenon of anti-A prozone and the non-specific blocking of Haemagglutination die to C1 complement fixation by IgG anti-A antibodies. Vox Sanguinis, 24, 408-419.
16. Voak, D. (1994) Validation of new technology for antibody detection. Vox Sanguinis, 67, suppl. 3. 113-115.
17. Voak, D., Downie, D.M., Darnborough, J., Haigh, T. &Fairham, S.A. (1980) A low ionic strength media for rapid antibody detection: optimum conditions and quality control. Medical and Laboratory Sciences, 37, 107-118.
18. Voak, D., Downie, M., Haigh, T. & Cook, N. (1992) Improved antiglobulin tests to detect difficult antibodies: detection of anti-Kell by LISS. Medical and Laboratory Sciences, 39, 363-370.
19. Voak, D., Downie, D.M., Moore, B.P.L., Ford, D.S., Engelfriet, C.P. & Case, J. (1986) Quality control of anti-human globulin tests: Use of replicate tests to improve performance. Biotest Bulletin, 1, 41-52.
20. Voak, D., Downie, D.M., Moore, B.P.L., Ford, D.S., Engelfriet, C.P. & Case, J. (1988) Replicate tests for the detection and correction of errors in anti-human globulin (AHG) test: optimum conditions and quality control. Haematologica, 21, 3-16.