Purification, characterization and conformational analysis of a haemorrhagin from the venom of Agkistrodon acutus

Toxicon

Volumn 35, Issue 2, 1997, Pages 283-292

  Zhu, Zhongliang ^a   Gong, Weimin ^a   Zhu, Xueyong ^a   Teng, Maikun ^a   Niu, Liwen ^a  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

[No Author keywords available]

Indexed keywords

SNAKE VENOM; TOXIN;

ANIMAL MODEL; ARTICLE; BLEEDING; CIRCULAR DICHROISM; CONTROLLED STUDY; DRUG PURIFICATION; DRUG STRUCTURE; DRUG TOXICITY; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LETHALITY; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SECONDARY STRUCTURE; RABBIT;

AGKISTRODON; ANIMALS; CIRCULAR DICHROISM; CROTALID VENOMS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MICE; RABBITS;

AGKISTRODON; ANIMALIA; DEINAGKISTRODON ACUTUS; ORYCTOLAGUS CUNICULUS; SERPENTES;

EID: 0031081675     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(96)00122-5     Document Type: Article

References (15)

1. Bjarnason, J. B. and Fox, J. W. (1994) Hemorrhagic metalloproteinases from snake venoms. Pharmac. Ther. 62, 325-372.
2. Bjarnason, J. B. and Fox, J. W. (1995) Snake venom metalloendopeptidases: reprolysins. Meth. Enzymol. 248, 345-368.
3. Bjarnason, J. B. and Tu, A. T. (1978) Hemorrhagic toxins from western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin. Biochemistry 17, 3395-3404.
4. Chen, Y. H., Yang, J. T. and Chau, K. H. (1974) Determination of the helix and β-form of proteins in aqueous solution by circular dichroism. Biochemistry 13, 3350-3359.
5. Hite, L. A., Jia, L. G., Bjarnason, J. B. and Fox, J. W. (1994) cDNA sequence for four snake venom metalloproteinases: structure, classification and their relationship to mammalian reproductive proteins. Arch. Biochem. Biophys. 38, 182-191.
6. Kondo, H., Kondo, S., Ikezawa, H., Murata, R. and Ohsake, A. (1960) Studies on the quantitative method for determination of hemorrhagic activity of Habu snake venom. Jpn. J. med. Sci. Biol. 13, 43-51.
7. Litchfield, J. R. and Wilcoxon, F. (1949) A simplified method of evaluating dose-effect experiment. J. Pharmac. exp. Ther. 96, 99-113.
8. Mori, N., Nikai, T. and Sugihara, H. (1984) Purification of a proteinase (Ac-5) and characterization of hemorrhagic toxins from the venom of the hundred-pace snake (Agkistrodon acutus). Toxicon 22, 451-461.
9. Nikai, T., Sugihara, H. and Tanaka, T. (1977) Enzymochemical studies on snake venoms - II. Purification of lethal protein Ac1-proteinase in the venom of Agkistrodon acutus. J. pharm. Soc. Jpn 97, 507-514.
10. Ovadia, M. (1978) Isolation and characterization of three hemorrhagic factors from the venom of Vipera palaestinae. Toxicon 16, 479-487.
11. Ovadia, M. (1987) Isolation and characterization of a hemorrhagic factor from the venom of the snake Atractaspis engaddensis (Atractasps didae). Toxicon 25, 621-630.
12. Van der Walt, S. J. and Joubert, F. J. (1971) Studies on puff adder (Bitis arietans) venom: I. Purification and properties of protease A. Toxicon 9, 153-161.
13. Xu, X., Wang, C., Liu, J. and Lu, Z. (1981) Purification and characterization of hemorrhagic components from Agkistrodon acutus (hundred pace snake) venom. Toxicon 19, 633-644.
14. Zhang, J. J., Chen, Z. X., He, Y. Z. and Xu, X. (1984) Effect of calcium on proteolytic activity and conformation of hemorrhagic toxin I from five pace snake (Agkistrodon acutus) venom. Toxicon 22, 931-935.
15. Zhu, Z., Gong, W., Niu, L., Teng, M. and He, H. (1996) Crystallization and preliminary X-ray diffraction study of hemorrhagin IV from snake venom of Agkistrodon acutus. Acta cryst. D52, 407-408.