In experimental diabetes the decrease in the eye of lens carnitine levels is an early important and selective event

Experimental Eye Research

Volumn 64, Issue 2, 1997, Pages 195-201

  Pessotto, P ^a   Liberati, R ^a   Petrella, O ^a   Romanelli, L ^b   Calvani, M ^a   Peluso, G ^c  

^a Sigma Tau SpA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c CNR   (Italy)

Author keywords

carnitine; diabetes; lens; rabbit; rat

Indexed keywords

ALLOXAN; CARNITINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DIABETES MELLITUS; LENS; MALE; NONHUMAN; PRIORITY JOURNAL; RABBIT; RAT;

EID: 0031080421     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.1996.0188     Document Type: Article

References (50)

1. Angelini, C., Martinuzzi, A. and Vergani, L. (1992). Treatment with L-Carnitine of the infantile and adult form of primary carnitine deficiency. In L-Carnitine and its role in medicine : from function to therapy. Eds. Ferrari, R., Di Mauro, S. and Sherwood, G. Pp. 139-53. Academic Press: London.
2. Aoshima, S., Fujisawa, S. and Kobayashi, A. (1993). Changes in the subcellular distribution of free carnitine and its acyl derivatives in diabetic rat hearts following treatment with L-carnitine. Jpn. Heart J. 34, 763-72.
3. Arduini, A., Mancinelli, G., Radatti, G., Dottori, S., Molajoni, F. and Ramsay, R. R. (1992). Role of carnitine and carnitine palmitoyltransferase as integral components of the pathway for membrane phospholipid fatty acid turnover in intact human erythrocytes. J. Biol. Chem. 267, 12673-81.
4. Beswick, H. T. and Harding, J. J. (1987). Conformational changes induce in lens α-and γ-crystallins by modification with glucose 6-phosphate. Biochem. J. 246, 761-9.
5. Bhuyan, K. C. and Bhuyan, D. K. (1984). Molecular mechanism of cataractogenesis (3): toxic metabolites of oxygen as initiators of lipid peroxidation and cataracts. Curr. Eye Res. 3, 67-81.
6. Bieber, L. L. (1988). Carnitine. Ann. Rev. Biochem. 57, 261-83.
7. Bing, R. J. (1965). Cardiac metabolism. Physiol. Rev. 45, 171-213.
8. Blakytny, R. and Harding, J. J. (1992). Prevention of cataract in diabetic rats by aspirin, paracetamol (acetaminophen) and ibuprofen. Exp. Eye Res. 54, 509-18.
9. Brass, E. P. (1992). Carnitine transport. In L-Carnitine and its role in medicine, from function to therapy. (Eds Ferrari, R., Di Mauro, S. and Sherwood, G.). Pp. 21-36. Academic Press: London.
10. Bremer, J. (1962). Carnitine in intermediary metabolism. Reversible acetylation of carnitine by mitochondria. J. Biol. Chem. 237, 2228-31.
11. Bucala, R., Manabe, S., Urban, R. C. and Cerami, A. (1985). Nonenzymatic modification of lens crystallins by prednisolone induces sulfhydryl oxidation and aggregate formation: in vitro and in vivo studies. Exp. Eye Res. 41, 353-63.
12. Cederblad, G. and Lindstedt, S. A. (1972). A method for the determination of carnitine in the picomole range. Clin. Chim. Acta 37, 235-43.
13. Dhalla, N. S., Dixon, I. M. C., Shah, K. R. and Ferrari, R. (1992). Beneficial effects of L-carnitine and derivatives on heart membranes in experimental diabetes. In L-carnitine and its role in medicine : from function to therapy (Eds Ferrari, R., Di Mauro, S. and Sherwood, G.). Pp. 411-26. Academic Press: London.
14. Di Mattio, J. and Zadunaisky, J. A. (1981). Glucose transport into the ocular compartments of the rat. Exp. Eye Res. 32, 517-32.
15. Fogle, P. J. and Bieber, L. L. (1979). Effect of streptozotocin on carnitine and carnitine acyltransferases in rat heart, liver, and kidney. Biochem. Medicine 22, 119-26.
16. Forloni, G., Angeretti, N. and Smiroldo, S. (1994). Neuro-protective activity of acetyl-L-carnitine: studies in vitro. J. Neuroscience Res. 37, 92-96.
17. Fritz, I. B. (1959). Action of carnitine on long chain fatty acid oxidation by liver. Am. J. Physiol. 197, 297-304.
18. Harding, J. J. and Van Heyningen, R. (1988). Drugs, including alcohol, that act as risk factors for cataract, and possible protection against cataract by aspirin-like analgesics and cyclopenthiazide. Br. J. Ophtalmol. 72, 809-14.
19. Harding, J. J. (1985). Nonenzymatic covalent posttranslational modification of proteins in vivo. Adv. Prot. Chem. 37, 247-334.
20. Harding, J. J. and Rixon, K. C. (1980). Carbamylation of lens proteins: a possible factor in cataractogenesis in some tropical countries. Exp. Eye Res. 31, 567-71.
21. Huby, R. and Harding, J. J. (1988). Non-enzymic glycosylation (glycation) of lens proteins by galactose and protection by aspirin and reduced glutathione. Exp. Eye Res. 47, 53-9.
22. Hülsmann, W. C., Siliprandi, D., Ciman, M. and Siliprandi, N. (1964). Effect of carnitine on the oxidation of α-oxoglutarate to succinate in the presence of acetoacetate or pyruvate. Biochem. Biophys. Acta 93, 166-8.
23. Hülsmann, W. C., Peschechera, A. and Arrigoni-Martelli, E. (1994). Carnitine and cardiac interstitium. Cardioscience 5, 67-72.
24. Kinoshita, J. H. (1974). Mechanisms initiating cataract formation. Invest. Ophthalmol. 13, 713-24.
25. Lamprecht, W. and Heinz, F. (1984). Pyruvate. In Methods of enzymatic analysis, 3rd ed. (Ed. Bergmeyer, H. U.) Vol. VI. Pp. 570-7. Verlag Chemie: Weinheim.
26. Lopaschuk, G. D. (1992). Effects of carnitine and carnitine acyltransferase inhibition on energy substrate utilization in the intact heart. In L-Carnitine and its role in medicine: from function to therapy (Eds Ferrari, R., Di Mauro, S. and Sherwood, G.). Pp. 403-10. Academic Press: London.
27. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). A reference base and system for analysis of muscle constituents. J. Biol. Chem. 182, 501-8.
28. Malone, J. I., Benford, S. A. and Malone, J., Jr. (1993). Taurine prevents galactose-induced cataracts. J. Diabetic Complications 7, 44-8.
29. Markwell, M. A. K., Haas, S. M., Bieber, L. L. and Tolbert, N. E. (1978). A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87, 206-10.
30. McGarry, J. D. and Foster, D. W. (1976). An improved and simplified radioisotopic assay for the determination of free and esterified carnitine. J. Lipid Res. 17, 277-81.
31. Mellamby, J. and Williamson, D. H. (1974). Acetoacetate. In Methods of enzymatic analysis, 2nd ed. (Ed. Bergmeyer, H. U.). Vol. 4. Pp. 1840-3. Verlag Chemie: Weinheim.
32. Noll, E. (1984). L-(+)-Lactate. In Methods of enzymatic analysis, 3rd ed. (Ed. Bergmeyer, H. U.). Vol. VI. Pp. 582-8. Verlag Chemie: Weinheim.
33. Paul, H. S. and Adibi, S. A. (1978). Effect of carnitine on branched-chain amino acid oxidation by liver and skeletal muscle. Am. J. Physiol. 234, 494-9.
34. Paulson, D. J., Schmidt, M. J., Traxler, J. S., Ramacci, M. T. and Shug, A. L. (1984). Improvement of myocardial function in diabetic rats after treatment with L-carnitine. Metabolism 33, 358-63.
35. Perry, R. E., Swamy, M. S. and Abraham, E. C. (1987). Progressive changes in lens crystallin glycation and high-molecular-weight aggregate formation leading to cataract development in streptozotocin-diabetic rats. Exp. Eye Res. 44, 269-82.
36. Pessotto, P., Valeri, P. and Arrigoni-Martelli, E. (1994). The presence of L-Carnitine in ocular tissues of the rabbit. J. Ocul. Pharmacol. 10, 643-51.
37. Philipson, B. (1969). Distribution of protein within the normal rat lens. Invest. Ophtalmol. 8, 258-70.
38. Qin, W., Smith, J. B. and Smith, D. L. (1993). Reaction of aspirin with cysteinyl residues of lens γ-crystallins; a mechanism for the proposed anticataract effect of aspirin. Biochim. Biophys. Acta 1181, 103-10.
39. Rao, G. N. and Cotlier, E. (1988). Aspirin prevents the nonenzymatic glycosylation and carbamylation of the human eye lens crystallins in vitro. Biochem. Biophys. Res. Comm. 151, 991-6.
40. Rebouche, C. J. and Engel, A. G. (1984). Kinetic compartmental analysis of carnitine, metabolism in the human carnitine deficiency syndromes: evidence for alterations in tissue carnitine transport. J. Clin. Invest. 73, 857-67.
41. Reddi, A. S., Jyothirmayi, G. N., De Angelis, B., Frank, O. and Baker, H. (1991). Effect of short and long-term diabetes on carnitine and myo-inositol in rats. Comp. Biochem. Physiol. 98, 39-42.
42. Riley, M. L. and Harding, J. J. (1993). The reaction of malondialdehyde with lens proteins and the protective effect of aspirin. Biochim. Biophys. Acta 1158, 107-12.
43. Stevens, V. J., Rouzer, C. A., Monnier, V. M. and Cerami, A. (1978). Diabetic cataract formation: potential role of glycosylation of lens crystallins. Proc. Natl. Acad. Sci. U.S.A. 75, 2918-22.
44. Swamy, M. S. and Abraham, E. C. (1989). Inhibition of lens crystallin glycation and high molecular weight aggregate formation by aspirin in vitro and in vivo. Invest. Ophthalmol. Vis. Sci. 30, 1120-6.
45. Visioli, O., Pasini, E., De Giuli, F. and Ferrari, R. (1992). Molecular mechanism of action of L-Carnitine in treatment of myocardial disorders at the experimental level. In L-carnitine and its role in medicine: from function to therapy (Eds Ferrari, R., Di Mauro, S. and Sherwood, G.). Pp. 237-63. Academic Press: London.
46. Wieland, O. H. (1984). Glycerol. U.V-method. In Methods of enzymatic analysis, 3rd ed. (Ed. Bergmeyer, H. U.). Vol. VI. Pp. 504-10. Verlag Chemie: Weinheim.
47. Williamson, D. H. and Mellamby, J. (1974). D-(-)-3-Hydroxybutyrate. In Methods of enzymatic analysis, 2nd ed. (Ed. Bergmeyer, H. U.). Vol. 4. Pp. 1836-9. Verlag Chemie: Weinheim.
48. Williamson, J. R., Chang, K., Frangos, M., Hasan, K. S., Ido, Y., Kawamura, T., Nyengaard, J. R., Van Den Enden, M., Kilo, C. and Tilton, R. G. (1993). Hyperglycemic pseudohypoxia and diabetic complications. Diabetes 42, 801-13.
49. Yeh, L. A. and Ashton, M. A. (1990). The increase in lipid peroxidation in diabetic rat lens can be reversed by oral sorbinil. Metabolism 39, 619-22.
50. Zhao, Z. H., Watschinger, B., Brown, C. D., Beyer, M. M. and Friedman, E. A. (1987). Variations of susceptibility to alloxan induced diabetes in the rabbit. Horm. Metabol. Res. 19, 534-7.