The role of Gi-proteins and β-adrenoceptors in the age-related decline of contraction in guinea-pig ventricular myocytes

Journal of Molecular and Cellular Cardiology

Volumn 29, Issue 2, 1997, Pages 439-448

  Ferrara, Nicola ^b   Böhm, Michael ^a   Zolk, Oliver ^a   O'Gara, Peter ^b   Harding, Sian E ^b  

^a UNIVERSITY HOSPITAL OF COLOGNE   (Germany)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

Author keywords

Adenosine; Ageing; Cardiomyocyte; G protein; Guinea pig

Indexed keywords

ADENOSINE; ADENOSINE DIPHOSPHATE; BETA ADRENERGIC RECEPTOR; CALCIUM ION; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOPRENALINE; PERTUSSIS TOXIN;

AGING; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GUINEA PIG; HEART MUSCLE CELL; HEART VENTRICLE WALL; MUSCLE FIBER CONTRACTION; MUSCLE RELAXATION; NONHUMAN; PRIORITY JOURNAL; SARCOMERE LENGTH; STATISTICAL ANALYSIS; VELOCITY;

EID: 0031080127     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1996.0397     Document Type: Article

References (34)

1. BAZAN A, VAN DE VELDE E, FRAEYMAN N, 1994. Effect of age on beta-receptors, Gs alpha-and Gi alpha-proteins in rat heart. Biochem Pharmacol 48: 479-486.
2. BÖHM M, GIERSCHIK P, JAKOBS KH, PIESKE B, SCHNABEL P, UNGERER M, ERDMANN E, 1990. Increase of Gi alpha in human hearts with dilated but not ischemic cardiomyopathy. Circulation 82: 1249-1265.
3. BÖHM M, LARISCH K, ERDMANN E, CAMPS M, JAKOBS K, GIERSCHIK P, 1991. Failure of [32P]ADP-ribosylation by pertussis toxin to determine Gi alpha content in membranes from various human tissues. Improved radioimmunological quantification using the 125I-labelled C-terminal decapeptide of retinal transducin. Biochem J 277: 223-229.
4. BÖHM M, GIERSCHIK P, KNORR A, LARISCH K, WEISMANN K, ERDMANN E, 1992. Desensitization of adenylate cyclase and increase of Gi alpha in cardiac hypertrophy due to acquired hypertension. Hypertension 20: 103-112.
5. BÖHM M, DORNER H, HTUN P, LENSCHE H, PLATT D, ERDMANN E, 1993a. Effects of exercise on myocardial adenylate cyclase and Gi alpha expression in senescence. Am J Physiol 264: H805-H814.
6. BÖHM M, UNGERER M, ERDMANN E, 1993b. Adenosine receptors in the human heart: pharmacological characterisation in nondiseased and cardiomyopathic tissue. Drug Dev Res 28: 268-276.
7. BÖHM M, ESCHENHAGEN T, GIERSCHIK P, LARISCH K, LENSCHE H, MENDE U, SCHMITZ W, SCHNABEL P, SCHOLZ H, STEINEATH M, ERDMANN E, 1994a. Radioimmunochemical quantification of Gia in right and left ventricles from patients with ischemic and dilated cardiomyopathy and predominant left ventricular failure. J Mol Cell Cardiol 26: 133-149.
8. BÖHM M, REIGER B, SCHWINGER RHG, ERDMANN E, 1994b. cAMP concentrations, cAMP dependent protein kinase activity, and phospholamban in non-failing and failing myocardium. Cardiovasc Res 28: 1713-1719.
9. BÖHM M, KIRCHMAYR R, GIERSCHIK P, ERDMANN E, 1995. Increase of myocardial inhibitory G-proteins in catecholamine-refractory septic shock or in septic multi-organ failure. Am J Med 98: 183-186.
10. BRODDE OE, ZERKOWSKI HR, SCHRANZ D, BROEDE-SITZ A, MICHEL-REHER M, SCHÄFER-BEISENBUSCH E, PIOTROWSKI JA, OELERT H, 1995. Age-dependent changes in the beta-adrenoceptor-G-protein(s)-adenylyl cyclase system in human right atrium. J Cardiovasc Pharmacol 26: 20-26.
11. BROWN LA, HARDING SE, 1992. The effect of pertussis toxin on β-adrenoceptor responses in isolated cardiac myocytes from noradrenaline-treated guinea-pigs and patients with cardiac failure. Br J Pharmacol 106: 115-122.
12. CLARK RB, KUNKEL MW, FRIEDMAN J, GOKA TJ, JOHNSON JA, 1988. Activation of cAMP-dependent protein kinase is required for heterologous desensitization of adenylyl cyclase in S49 wild-type lymphoma cells. Proc Natl Acad Sci USA 85: 1442-1446.
13. DANIELSEN W, VON DER LEYEN H, MEYER W, NEUMANN J, SCHMITZ W, SCHOLZ H, STARBATTY J, STEIN B, DÖRING V, KALMAR P, 1989. Basal and isoprenaline-stimulated cAMP content in failing versus nonfailing human cardiac preparations. J Cardiovasc Pharmacol 14:171-173.
14. DAVIES CH, FERRARA N, HARDING SE, 1995. Beta-adrenoceptor function changes with age of subject in myocytes from non-failing ventricle. Cardiovasc Res 31: 152-156.
15. DEL MONTE F, O'GARA P, POOLE-WILSON PA, YACOUB MH, HARDING SE, 1995. Cell geometry and contractile abnormalities of myocytes from failing human left ventricle. Cardiovasc Res 30: 281-290.
16. ESCHENHAGEN T, MENDE U, DIEDERICH M, NOSE M, SCHMITZ W, SCHOLZ H, SCHULTE AM, ESCH J, WARNHOLTZ A, SCHÄFER H, 1992. Long term beta-adrenoceptor-mediated up-regulation of Gi alpha and G(o) alpha mRNA levels and pertussis toxin-sensitive guanine nucleotide-binding proteins in rat heart. Mol Pharmacol 42: 773-783.
17. FELDMAN AM, CATES AE, VEAZEY WB, HERSHBERGER RE, BRISTOW MR, BAUGHMAN KL, BAUMGARTNER WA, VAN DOP C, 1988. Increase of the 40,000-mol wt pertussis toxin substrate (G protein) in the failing human heart. J Clin Invest 82: 189-197.
18. FELDMAN AM, JACKSON DG, BRISTOW MR, CATES AE, VAN DOP C, 1991. Immunodetectable levels of the inhibitory guanine neucleotide-binding regulatory proteins in failing human heart: discordance with measurements of adenylate cyclase activity and levels of pertussis toxin substrate. J Mol Cell Cardiol 23: 439-452.
19. FERRARA N, O'GARA P, WYNNE DG, BROWN LA, DEL MONTE F, POOL-WILSON PA, HARDING SE, 1995. Decreased contractile responses to isoproterenol in isolated cardiac myocytes from ageing guinea-pigs. j Mol Cell Cardiol 27: 1141-1150.
20. GALBO H, RICHTER EA, HOLST JJ, CHRISTENSEN NJ, 1977. Diminished hormonal responses to exercise in trained rats. J Appl Physiol 43: 953-958.
21. GIERSCHIK P, CODINA J, SIMONS C, BIRNBAUMER L, SPIEGEL AM, 1985. Antisera against a guanine nucleotide binding protein from retina cross-react with the β-subunit of the adenylyl cyclase-associated guanine nucleotide binding protein, Ns and Ni. Proc Natl Acad Sci USA 82: 727-731.
22. GOLDSMITH P, GIERSCHIK P, MILLIGAN G, UNSON CG, VINITSKY R, MALECH HI, SPIEGEL AM, 1987. Antibodies direct against synthetic peptides distinguish between GTP-binding proteins in neutrophils and brain. J Biol Chem 262: 14683-14688.
23. GUDMUNDSDÖTTIR E, BENEDIKTSDÖTTIR VE, GUDBJARNASON S, 1991. Combined effects of age and dietary fat on beta 1-receptors and Ca2 + channels in rat hearts. Am J Physiol 260: H66-H72.
24. HARDING SE, JONES SM, O'GARA P, DEL MONTE F, VESCOVO G, POOLE-WILSON PA, 1992. Isolated ventricular myocytes from failing and non-failing human heart; the relation of age and clinical status of patients to isoproterenol response. J Mol Cell Cardiol 24: 549-564.
25. HARDING SE, DAVIES CH, WYNNE DG, POOLE-WILSON PA, 1994. Contractile characteristics and response to agonists in myocytes from failing human heart. Eur Heart J 15 (Suppl D): 35-36.
26. LÄMMLI UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
27. LOWRY OH, ROSENBROUGH NJ, FARR AL, RANDALL RJ, 1951. Protein measurement with the Folin phenol reagent. j Biol Chem 193: 265-270.
28. MÜLLER FU, BOHELER KR, ESCHENHAGEN T, SCHMITZ W, SCHOLZ H, 1993. Isoprenaline stimulates gene transcription of the inhibitory G protein alpha-subunit Gi alpha-2 in rat heart. Circ Res 72: 696-700.
29. REGITZ-ZAGROSEK V, HERTRAMPF R, STEFFEN C, HIDEBRANDT A, FLECK E, 1994. Myocardial cyclic AMP and norepinephrine content in human heart failure. Eur Heart J 15 (Suppl. D): 7-13.
30. REITHMANN C, GIERSCHIK P, MÜLLER U, WERDAN K, JAKOBS KH, 1990. Pseudomonas exotoxin A presents beta-adrenoceptor-induced upregulation of Gi protein alpha-subunits and adenylyl cyclase desensitization in rat heart muscle cells. Mol Pharmacol 37: 631-638.
31. RODEHEFFER RJ, GERSTENBLITH G, BECKER LC, FLEG JL, WEISFELDT ML, LAKATTA EG, 1984. Exercise cardiac output is maintained with advancing age in healthy human subjects: cardiac dilatation and increased stroke volume compensate for a diminished heart rate. Circulation 69: 203-213.
32. VON DER LEYEN H, MENDE U, MEYER W, NEUMANN J, NOSE M, SCHMITZ W, SCHOLZ H, STARBATTY J, STEIN B, WENZLAFF H, DÖRING V, KALMAR P, HAVERICH A, 1991. Mechanism underlying the reduced positive inotropic effects of the phosphodiesterase III inhibitors pimobendan, adibendan and saterinone in failing as compared to nonfailing human cardiac muscle preparations. Naunyn Schmiedebergs Arch Pharmacol 344: 90-100.
33. WHITE M, RODEN R, MINOBE W, KHAN MF, LARRABEE P, WOLLMERING M, PORT JD, ANDERSON F, CAMPBELL D, FELDMAN AM, et al, 1994. Age-related changes in beta-adrenergic neuroeffector systems in the human heart. Circulation 90: 1225-1238.
34. ZIEGLER MG, LAKE CR, KOPIN IJ, 1976. Plasma noradrenaline increases with age. Nature 261: 333-335.