Expression of functional eIF-4E(human) : Purification, detailed characterization, and its use in isolating eIF-4E binding proteins

Protein Expression and Purification

Volumn 9, Issue 1, 1997, Pages 53-60

  Hagedorn, Curt H ^a,b   Spivak Kroizman, Taly ^a   Friedland, Diana E ^c   Goss, Dixie J ^c   Xie, Yiping ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CITY UNIVERSITY OF NEW YORK   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; MAMMALIA; POLIOVIRUS;

EID: 0031078409     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0661     Document Type: Article

References (51)

1. 1. Shatkin, A. J. (1985) mRNA cap binding proteins: Essential factors for initiating translation. Cell 40, 223-224.
2. 2. Sonenberg, N. (1994) Regulation of translation and cell growth by eIF-4E. Biochimie 76, 839-846.
3. 3. Rhoads, R. E., Joshi, B., and Minich, W. B. (1994) Participation of initiation factors in the recruitment of mRNA to ribosomes. Biochimie 76, 831-838.
4. 4. Hershey, J. W. (1994) Expression of initiation factor genes in mammalian cells. Biochimie 76, 847-852.
5. 5. Sonenberg, N., Morgan, M. A., Merrick, W. C., and Shatkin, A. J. (1978) A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5′-terminal cap in mRNA. Proc. Natl. Acad. Sci. USA 75, 4843-4847.
6. 7G-cap binding protein with different effects on capped mRNA translation in extracts of uninfected and poliovirus-infected HeLa cells. J. Biol. Chem. 256, 7691-7694.
7. 7. Ray, B. K., Lawson, T. G., Kramer, J. C., Cladaras, M. H., Grifo, J. A., Abramson, R. D., Merrick, W. C., and Thach, R. E. (1985) ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors. J. Biol. Chem. 260, 7651-7658.
8. 8. Jaramillo, M., Dever, T. E., Merrick, W. C., and Sonenberg, N. (1991) RNA unwinding in translation: Assembly of helicase complex intermediates comprising eukaryotic initiation factors eIF-4E and eIF-4B. Mol. Cell. Biol. 11, 5992-5997.
9. 9. Joshi, B., Yan, R., and Rhoads, R. E. (1994) In vitro synthesis of human protein synthesis initiation factor 4γ and its localization of 43 and 48 S initiation complexes. J. Biol. Chem. 269, 2048-2055.
10. 10. Buckley, B., and Ehrenfeld, E. (1987) The cap binding protein complex in uninfected and poliovirus-infected HeLa cells. J. Biol. Chem. 262, 13599-13606.
11. 11. Hellen, C. U., Facke, M., Krausslich, H. G., Lee, C. K., and Wimmer, E. (1991) Characterization of poliovirus 2A proteinase by mutational analysis: Residues required for autocatalytic activity are essential for induction of cleavage of eukaryotic initiation factor 4F polypeptide p220. J. Virol. 65, 4226-4231.
12. 12. Rychlik, W., Gardner, P. R., Vanaman, T. C., and Rhoads, R. E. (1986) Structural analysis of the messenger RNA cap-binding protein. Presence of phosphate, sulfhydryl, and disulfide groups. J. Biol. Chem. 261, 71-75.
13. 13. Duncan, R., Milburn, S. C., and Hershey, J. W. B. (1987) Regulated phosphorylation and low abundance of HeLa cell initiation factor eIF-4E suggest a role in translational control. J. Biol. Chem. 262, 380-388.
14. 14. McMullin, E. L., Haas, D.W., Abramson, R. D., Thach, R. E., Merrick, W. C., and Hagedorn, C. H. (1988) Identification of a protein kinase activity in rabbit reticulocytes that phosphorylates the mRNA cap binding protein. Biochem. Biophys. Res. Commun. 153, 340-346.
15. 15. Marino, M. W., Pfeffer, L. M., Guidon, P. T., Jr., and Donner, D. B. (1989) Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells. Proc. Natl. Acad. Sci. USA 86, 8417-8421.
16. 16. Morley, S. J., and Traugh, J. A. (1990) Differential stimulation of phosphorylation of initiation factors eIF-4E, eIF-4B, eIF-3 and ribosomal protein S6 by insulin and phorbol esters. J. Biol. Chem. 265, 10611-10616.
17. 17. Donaldson, R. W., Hagedorn, C. H., and Cohen, S. (1991) Epidermal growth factor or okadaic acid stimulates phosphorylation of eukaryotic initiation factor 4F. J. Biol. Chem. 266, 3162-3166.
18. 18. Bu, X., and Hagedorn, C. H. (1991) Platelet-derived growth factor stimulates phosphorylation of the 25 kDa mRNA cap binding protein (eIF-4E) in human lung fibroblasts. FEBS Lett. 283, 219-222.
19. 19. Huang, J., and Schneider, R. J. (1991) Adenovirus inhibition of cellular protein synthesis involves inactivation of cap binding protein. Cell 65, 271-280.
20. 20. Frederickson, R. M., Mushynski, W. E., and Sonenberg, N. (1992) Phosphorylation of translation initiation factor eIF-4E is induced in a ras-dependent manner during nerve growth factor mediated PC12 cell differentiation. Mol. Cell. Biol. 12, 1239-1247.
21. 21. Bu, X., Bondurant, M. C., and Hagedorn, C. H. (1996) Erythropoietin stimulates phosphorylation of eIF-4E and identification of a 37 kDa phosphoprotein that specifically binds mRNA caps in erythroblasts. Exp. Hematol. 24, 423-428.
22. 22. Rychlik, W., Rush, J. S., Rhoads, R. E., and Waechter, C. J. (1990) Increased rate of phosphorylation-dephosphorylation of the translational initiation factor eIF-4E correlates with the induction of protein and glycoprotein biosynthesis in activated B lymphocytes. J. Biol. Chem. 265, 19467-19471.
23. 23. Bu, X., Haas, D. W., and Hagedorn, C. H. (1993) Novel phosphorylation sites of eukaryotic initiation factor-4F and evidence that phosphorylation stabilizes interactions of the p25 and p220 subunits. J. Biol. Chem. 268, 4975-4978.
24. 24. Morley, S. J., Rau, M., Kay, J. E., and Pain, V. M. (1993) Increased phosphorylation of eukaryotic initiation factor 4α during early activation of T lymphocytes correlates with increased initiation factor 4F complex formation. Eur. J. Biochem. 218, 39-48.
25. 25. Makkinje, A., Xiong, H., Li, M., and Damuni, Z. (1995) Phosphorylation of eukaryotic protein synthesis initiation factor 4E by insulin-stimulated protamine kinase. J. Biol. Chem. 270, 14824-14828.
26. 26. Tuazon, P. T., Merrick, W. C., and Traugh, J. A. (1989) Comparative analysis of phosphorylation of translational initiation and elongation factors by seven protein kinases. J. Biol. Chem. 264, 2773-2777.
27. 27. Haas, D. W., and Hagedorn, C. H. (1992) Protein kinase C phosphorylates both serine and threonine residues of the mRNA cap binding protein eIF-4E. Second Messengers Phosphoproteins 14, 55-63.
28. 28. Bu, X., and Hagedorn, C. H. (1992) Phosphoprotein phosphatase 2A dephosphorylates eIF-4F and does not alter binding to the mRNA cap. FEBS Lett. 301, 15-18.
29. 29. Minich, W. B., Balasta, M. L., Goss, D. J., and Rhoads, R. E. (1994) Chromatographic resolution of in vivo phosphorylated and nonphosphorylated eukaryotic translation initiation factor eIF-4E: Increased cap affinity of the phosphorylated form. Proc. Natl. Acad. Sci. USA 91, 7668-7672.
30. 30. Lin, T-A., Kong, X., Haystead, T. A. J., Pause, A., Belsham, G., Sonenberg, N., and Lawrence, J. C. (1994) PHAS-I as a link between mitogen-activated protein kinase and translation initiation. Science 266, 653-656.
31. 31. Pause, A., Belsham, G. J., Gingas, A-C., Donze, O., Lin, T-A., Lawrence, J. C., and Sonenberg, N. (1994) Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5′-cap function. Nature 371, 762-767.'
32. 32. Graves, L. M., Bornfeldt, K. E., Argast, G. M., Krebs, E.G., Kong, X., Lin, T. A., and Lawrence, J. C. (1995) cAMP-and rapamycin-sensitive regulation of the association of eukaryotic initiation factor 4E and the translational regulator PHAS-I in aortic smooth muscle cells. Proc. Natl. Acad. Sci. USA 92, 7222-7226.
33. 33. Joshi, B., Cai, A-L., Keiper, B. D., Minich, W. B., Mendez, R., Beach, C. M., Stepinski, J., Stolarski, R., Darzynkiewicz, E., and Rhoads, R. E. (1995) Phosphorylation of eukaryotic protein synthesis initiation factor 4E at ser-209. J. Biol. Chem. 270, 14597-14603.
34. 34. Whalen, S. G., Gingras, A-C., Amankwa, L., Mader, S., Branton, P. E., Aebersold, R., and Sonenberg, N. (1996) Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins. J. Biol. Chem. 271, 11831-11837.
35. 35. Stirewalt, W. S., Low, R. B., and Slaiby, J. M. (1985) Insulin sensitivity and responsiveness of epitrochlearis and soleus muscles from fed and starved rats. Recognition of differential changes in insulin sensitivities of protein synthesis and glucose incorporation into glycogen. Biochem. J. 227, 355-362.
36. 36. Ashford, A. J., and Pain, V. N. (1986) Insulin stimulation of growth in diabetic rats. Synthesis and degradation of ribosomes and total tissue protein in skeletal muscle and heart. J. Biol. Chem. 261, 4066-4070.
37. 37. Baker, B. F., Miraglia, L., and Hagedorn, C. H. (1992) Modulation of eukaryotic initiation factor-4E binding to 5′-capped oligoribonucleotides by modified anti-sense oligonucleotides. J. Biol. Chem. 267, 11495-11499.
38. 38. Edery, I., Altmann, M., and Sonenberg, N. (1988) High-level synthesis in Escherichia coli of functional cap-binding eukaryotic initiation factor eIF-4E and affinity purification using a simplified cap-analog resin. Gene 74, 517-525.
39. 39. Edery, I., Chu, L. L., Sonenberg, N., and Pelletier, J. (1995) An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture). Mol. Cell. Biol. 15, 3363-3371.
40. 40. Friedland, D. E., Shoemaker, M. T., Xie, Y., Wang, Y., Hagedorn, C. H., and Goss, D. J. (1996) Identification of the cap-binding domain of human recombinant eukaryotic initiation factor 4E using a photoaffinity analog. Protein Sci. in Press.
41. 41. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89.
42. 42. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning - A Laboratory Manual." Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
43. 43. Faloona, F., and Mullis, K. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155, 335-350.
44. 44. Rychlik, W., Domier, L. L., Gardner, P. R., Hellmann, G. M., and Rhoads, R. E. (1987) Amino acid sequence of the mRNA cap-binding protein from human tissues. Proc. Natl. Acad. Sci. USA 84, 945-949.
45. 45. Haas, D. W., and Hagedorn, C. H. (1991) Casein kinase I phosphorylates the 25-kDa mRNA cap-binding protein. Arch. Biochem. Biophys. 284, 84-89.
46. 7GpppG to human protein synthesis initiation factor 4E. Biochemistry 28, 8078-8083.
47. 47. Carberry, S. E., Darzynkiewich, E., Stepinsky, J., Tahara, S. M., Rhoads, R. E., and Goss, D. J. (1990) A spectroscopic study of the binding of N-7-substituted cap analogues to human protein synthesis initiation factor 4E. Biochemistry 29, 3337-3341.
48. 48. Hagedorn, C. H., Tettelbach, W. H., and Panella, H. L. (1990) Development and characterization of polyclonal antibodies against a conserved sequence in the catalytic domain of protein kinases. FEB Lett. 264, 59-62.
49. 49. Stern, B. D., Wilson, M., and Jagus, R. (1993) Use of nonreducing SDS-PAGE for monitoring renaturation of recombinant protein synthesis initiation factor, eIF-4 alpha. Protein Expression Purif. 4, 320-327.
50. 50. Mick, S. J., Abramson, R. D., Ray, B. K., Merrick, W. C., Thach, R. E., and Hagedorn, C. H. (1988) Induction of eIF-4E phosphorylation by the addition of L-pyrroline-5-carboxylic acid to rabbit retculocyte lysate. FEBS Lett. 236, 484-488.
51. 51. Minich, W. B., Balasta, M. L., Goss, D. J., and Rhoads, R. E. (1994) Chromatographic resolution of in vivo phosphorylated and nonphosphorylated eukaryotic translation initiation factor eIF-4E: Increased cap affinity of the phosphorylated form. Proc. Natl. Acad. Sci. USA 91, 7668-7672.