Selective 15N labeling and direct observation by NMR of the active-site glutamine of Fe-containing superoxide dismutase

Journal of Biomolecular NMR

Volumn 9, Issue 2, 1997, Pages 201-206

  Vance, Carrie K ^a   Kang, Young M ^a   Miller, Anne Frances ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

15N; Glutamine; Selective labeling; Superoxide dismutase

Indexed keywords

GLUTAMINE; IRON; ISOENZYME; MANGANESE; NITROGEN; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE;

ARTICLE; BINDING SITE; CHEMISTRY; COMPARATIVE STUDY; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY;

BINDING SITES; CLONING, MOLECULAR; GLUTAMINE; IRON; ISOENZYMES; MAGNETIC RESONANCE SPECTROSCOPY; MANGANESE; NITROGEN ISOTOPES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SUPEROXIDE DISMUTASE;

EID: 0031064318     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018662421878     Document Type: Article

References (28)

1. Bertini, I. and Luchinat, C. (1986) NMR of Paramagnetic Molecules in Biological Systems, Benjamin Cummings, New York, NY, U.S.A.
2. Bertini, I., Luchinat, C. and Piccioli, M. (1994) Prog. NMR Spectrosc., 26, 91-139.
3. Beyer, J.W.F., Reynolds, J.A. and Fridovich, I. (1989) Biochemistry, 28, 4403-4409.
4. Carlioz, A. and Touati, D. (1986) EMBO J., 5, 623-630.
5. Carlioz, A., Ludwig, M.L., Stallings, W.C., Fee, J.A., Steinman, H.M. and Touati, D. (1988) J. Biol. Chem., 263, 1555-1562.
6. Cheng, H. and Markley, J.L. (1995) Annu. Rev. Biophys. Biomol. Struct., 24, 209-237.
7. Emptage, M.H., Xavier, A.V., Wood, J.M., Alsaadi, B.M., Moore, G.R., Pitt, R.C., Williams, R.J.P., Ambler, R.P. and Bartsch, R.G. (1981) Biochemistry, 20, 58-64.
8. Inubishi, T. and Becker, F.D. (1983) J. Magn. Reson., 51, 128-133.
9. Kiefer, L.L., Paterno, S.A. and Fierke, C.A. (1995) J. Am. Chem. Soc., 117, 6831-6837.
10. Lah, M.S., Dixon, M.M., Pattridge, K.A., Stallings, W.C., Fee, J.A. and Ludwig, M.L. (1995) Biochemistry, 34, 1646-1660.
11. Levy, G.C. and Lichter, R.L. (1979) Nitrogen-15 Nuclear Magnetic Resonance Spectroscopy, Wiley, New York, NY, U.S.A.
12. Metzler, D.E. (1977) Biochemistry: The Chemical Reactions of Living Cells, Academic Press, New York, NY, U.S.A.
13. Miller, A.-F., Papastavros, M.Z. and Redfield, A.G. (1992) Biochemistry, 31, 10208-10216.
14. Miller, A.-F. and Sorkin, D.L. (1997) Comm. Mol. Cell. Biophys., in press.
15. Ming, L.-J., Lynch, J.B., Holz, R.C. and Que Jr., L. (1994) Inorg. Chem., 33, 83-87.
16. Morris, G.A. and Freeman, R. (1979) J. Am. Chem. Soc., 101, 760-762.
17. Muchmore, D.C., McIntosh, L.P., Russell, C.B., Anderson, D.E. and Dahlquist, F.W. (1989) Methods Enzymol., 177, 44-73.
18. Oh, B.-H. and Markley, J.L. (1990) Biochemistry, 29, 4012-4017.
19. Ose, D.E. and Fridovich, I. (1979) Arch. Biochem. Biophys., 194, 360-364.
20. Parker, J. and Friesen, J.D. (1980) Mol. Gen. Genet., 177, 439-445.
21. Parker, M.W. and Blake, C.C.F. (1988) FEBS Lett., 229, 377-382.
22. Slykhouse, T.O. and Fee, J.A. (1976) J. Biol. Chem., 251, 5472-5477.
23. Sorkin, D.L. and Miller, A.-F. (1997) Biochemistry, in press.
24. Stallings, W.C., Pattridge, K.A., Strong, R.K. and Ludwig, M.L. (1984) J. Biol. Chem., 259, 10695-10699.
25. Stoddard, B.L., Howell, P.L., Ringe, D. and Petsko, G.A. (1990) Biochemistry, 29, 8885-8893.
26. Tate, S., Tate, N.U., Ravera, M.W., Jaye, M. and Inagaki, F. (1992) FEBS Lett., 297, 39-42.
27. Unger, S.W., Le Comte, J.T.J. and La Mar, G.N. (1985) J. Magn. Reson., 64, 521-526.
28. Yamakura, F., Kobayashi, K., Ue, H. and Konno, M. (1995) Eur. J. Biochem., 227, 700-706.