Isolation of catalase-negative Listeria monocytogenes strains from listeriosis patients and their rapid identification by anti-p60 antibodies and/or PCR

Journal of Clinical Microbiology

Volumn 35, Issue 1, 1997, Pages 179-183

  Bubert, Andreas ^a   Riebe, Joachim ^b   Schnitzler, Norbert ^c   Schönberg, Arno ^d   Goebel, Werner ^a   Schubert, Peter ^e  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b Gemeinschaftspraxis Labormedizin   (Germany)

^c RWTH AACHEN UNIVERSITY   (Germany)

^d Bundesinstitut F G   (Germany)

^e MERCK KGAA   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CATALASE; PROTEIN P60; UNCLASSIFIED DRUG;

ADULT; AGED; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CASE REPORT; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GERMANY; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; LISTERIA MONOCYTOGENES; LISTERIOSIS; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); LISTERIA MONOCYTOGENES; LISTERIOSIS MONOCYTOGENES; POSIBACTERIA;

EID: 0031059926     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.1.179-183.1997     Document Type: Article

References (26)

1. Bille, J., B. Catimel, E. Bannerman, C. Jacquet, M.-N. Yersin, I. Caniaux, D. Monget, and J. Recourt. 1992. API Listeria, a new and promising one-day system to identity Listeria isolates. Appl. Environ. Microbiol. 58:1857-1860.
2. Bubert, A., M. Kuhn, W. Goebel, and S. Köhler. 1992. Structural and functional properties of the p60 proteins from different Listeria species. J. Bacteriol. 174:8166-8171.
3. Bubert, A., P. Schubert, S. Köhler, R. Frank, and W. Goebel. 1994. Synthetic peptides derived from the Listeria monocytogenes p60 protein as antigens for the generation of polyclonal antibodies specific for secreted cell-free L. monocytogenes p60 proteins. Appl. Environ. Microbiol. 60:3120-3127.
4. Bubert, A., P. Schubert, S. Köhler, and W. Goebel. 1995. Specific immunological detection of all Listeria monocytogenes serotypes using antibodies raised against p60-derived synthetic peptides. p. 65-70. In Proceedings of the XII International Symposium on Problems of Listeriosis. PROMACO Conventions Pty. Ltd., Perth, Western Australia, Australia.
5. Bubert, A., S. Köhler, and W. Goebel. 1992. The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction. Appl. Environ. Microbiol. 58:2625-2632.
6. Deneer, H., and I. Boychuk. 1991. Species-specific identification of Listeria monocytogenes by DNA amplification. Appl. Environ. Microbiol. 57:606-609.
7. Furrer, B., U. Candrian, C. Hoefelein, and J. Luethy. 1991. Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. J. Appl. Bacteriol. 70:372-379.
8. Gentschev, I., Z. Sokolovic, S. Köhler, G. F. Krohne, H. Hof, J. Wagner, and W. Goebel. 1992. Identification of p60 antibodies in human sera and presentation of this listerial antigen on the surface of attenuated salmonellae by the HlyB-HlyD secretion system. Infect. Immun. 60:5091-5098.
9. Haas, A., K. Brehm, J. Kreft, and W. Goebel. 1991. Cloning, characterization, and expression in Escherichia coli of a gene encoding Listeria seeligeri catalase, a bacterial enzyme highly homologous to mammalian catalases. J. Bacteriol. 173:5159-5167.
10. Hof, H., T. Nichterlein, and M. Kretschmar. 1994. When are Listeria in foods a health risk. Trends Food Sci. Technol. 5:185-190.
11. Köhler, S., M. Leimeister-Wächter, T. Chakraborty, F. Lottspeich, and W. Goebel. 1990. The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect. Immun. 58: 1943-1950.
12. Kuhn, M., and W. Goebel. 1989. Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells. Infect. Immun. 57:55-61.
13. Kuhn, M., and W. Goebel. 1995. Molecular studies on the virulence of Listeria monocytogenes. Genet. Eng. 17:31-51.
14. Kyhse-Andersen, J. 1984. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J. Biochem. Biophys. Methods 10:203-209.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
16. Leblond-Francillard, M., J.-L. Gaillard, and P. Berche. 1989. Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo. Infect. Immun. 57:2569-2573.
17. McKellar, R. C. 1994. Use of the CAMP test for identification of Listeria monocytogenes. Appl. Environ. Microbiol. 60:4219-4225.
18. Ruhland, G. H., M. Hellwig, G. Wanner, and F. Fiedler. 1993. Cell-surface localisation of Listeria-specific protein p60-detection of Listeria cells by indirect immunofluorescence. J. Gen. Microbiol. 139:609-616.
19. Schubert, P., K. Kramer, and A. Bubert. 1996. Evaluation and application of Listeria monocytogenes specific antibodies, abstr. B-51, p. 44. In Abstracts of the International Association of Milk and Food Environmental Sanitarians 1996.
20. Schuchat, A., B. Swaminathan, and C. V. Broome. 1991. Epidemiology of human listeriosis. Clin. Microbiol. Rev. 4:169-183.
21. Seeliger, H. P. R., and D. Jones. 1986. Listeria, p. 1235-1245. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's manual of systematic bacteriology. The Williams & Wilkins Co., Baltimore, Md.
22. Seeliger, H. P. R., and K. Höhne. 1979. Serotyping of Listeria monocytogenes and related species. Methods Microbiol. 13:31-49.
23. Swartz, M. A., D. F. Welch, R. D. Narayanan, and R. A. Greenfield. 1991. Catalase-negative Listeria monocytogenes causing meningitis in an adult. Am. J. Clin. Pathol. 96:130-133.
24. Welch, D. F. 1987. Role of catalase and superoxide dismutase in the virulence of Listeria monocytogenes. Ann. Inst. Pasteur Microbiol. 138:241-284.
25. Wernars, K., K. Heuvelman, S. Notermans, E. Domann, M. Leimeister-Wächter, and T. Chakraborty. Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp. Appl. Environ. Microbiol. 58:765-768.
26. Wuenscher, M., S. Köhler, A. Bubert, U. Gerike, and W. Goebel. 1993. The iap gene of Listeria monocytogenes is essential for cell viability and its gene product p60 has bacteriolytic activity. J. Bacteriol. 175:3491-3501.