Differential expression of low Mr GTP-binding proteins in human megakaryoblastic leukemia cell line, MEG-01, and their possible involvement in the differentiation process

Thrombosis and Haemostasis

Volumn 77, Issue 2, 1997, Pages 368-375

  Nagata, Koh Ichi ^a   Okano, Yukio ^a   Nozawa, Yoshinori ^a  

^a GIFU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; MESSENGER RNA; PHORBOL 13 ACETATE 12 MYRISTATE; TRITON X 100;

ARTICLE; CELL DIFFERENTIATION; CELL MEMBRANE; CONTROLLED STUDY; CYTOSKELETON; CYTOSOL; GENE AMPLIFICATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LEUKEMIA CELL LINE; MEGAKARYOBLAST; MEGAKARYOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0031058752     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0038-1655970     Document Type: Article

References (49)

1. Valencia A, Chardin P, Wittinghofer A, Sander C. The ras protein family: evolutionary tree and role of conserved amino acids. Biochemistry 1991; 30: 4637-48.
2. Takai Y, Kaibuchi K, Kikuchi A, Kawata M. Small GTP-binding proteins. Int Rev Cytol 1992; 133: 187-230.
3. Barbacid M. ras Genes. Ann Rev Biochem 1987; 56: 779-827.
4. Kaziro Y, Itoh H, Kozasa T, Nakafuku M, Satoh T. Structure and function of signal-transducing GTP-binding proteins. Ann Rev Biochem 1991; 60: 349-400.
5. Ogura M, Morishima Y, Ohno R, Kato Y, Hirabayashi N, Nagura H, Saito H. Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome. Blood 1985; 66: 1384-92.
6. Ogura M, Morishima Y, Okumura M, Hotta T, Takamoto S, Ohno R, Hirabayashi N, Nagura H, Saito H. Functional and morphological differentiation induction of a human megakaryoblastic leukemia cell line (MEG-01s) by phorbol diesters. Blood 1988; 72: 49-60.
7. C-SRC to the cytoskeleton during platelet aggregation. EMBO J 1992; 11: 855-64.
8. Nagata K, Itoh H, Katada T, Takenaka K, Ui M, Kaziro Y, Nozawa Y. Purification, identification, and characterization of two GTP-binding proteins with molecular weights of 25,000 and 21,000 in human platelet cytosol: one is the rap1/smg21/Krev-1 protein and the other is a novel GTP-binding protein. J Biol Chem 1989; 264: 17000-5.
9. Nagata K, Nozawa Y. Subcellular localization of a low Mr GTP-binding protein, c25KG, in resting and stimulated human platelets. Biochem Biophys Res Commun 1993; 195: 1081-8.
10. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1988; 162: 156-9.
11. Gyllensten UB, Erlich HA. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci USA 1988; 85: 7652-6.
12. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
13. Nagata K, Hattori T, Hachiya T, Takahashi T, Nozawa Y. Purification and characterization of a low Mr GTP-binding protein, c25KG, from human platelet membranes. Biochem Biophys Acta 1992; 1160: 193-8.
14. Bradford MM. A rapid and simple method for the quantitation of quantities of protein utilizing the principle of protein-dye. Analyt Biochem 1976; 72: 248-54.
15. Nagata K, Nozawa Y. Purification and characterization of two GTP-binding proteins of 22 kDa from human platelet membranes. FEBS Let 1988; 237: 113-7.
16. Ohmori T, Kikuchi A, Yamamoto K, Kim S, Takai Y. Small molecular weight GTP-binding proteins in human platelet membranes: Purification and characterization of a novel GTP-binding protein with a molecular weight of 22,000. J Biol Chem 1989; 246: 1877-81.
17. Nagata K, Satoh T, Itoh H, Kozasa T, Okano Y, Doi T, Kaziro Y, Nozawa Y. The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library. FEBS Lett 1990; 275: 29-32.
18. Farrell FX, Ohmstede CA, Reep BR, Lapetina EG. cDNA sequence of a new ras-related gene (rap2b) isolated from human platelets with sequence homology to rap2. Nucleic Acids Res 1990; 18: 4281.
19. Karniguian A, Zahraoui A, Tavitian A. Identification of small GTP-binding proteins in human platelets: Thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins. Proc Natl Acad Sci USA 1993; 90: 7647-51.
20. Nemoto Y, Namb T, Teru-uchi T, Ushikubi F, Morii N, Narumiya S. A rho gene product in human platelets: I Identification of the platelet substrate for botulinum C3 ADP-ribosyltransferase as rhoA protein. J Biol Chem 1992; 267: 20916-20.
21. Narumiya S, Morii N. rho gene products, botulinom C3 exoenzyme and cell adhesion. Cell Signal 1993; 5: 9-19.
22. Morii N, Teru-uchi T, Tominaga T, Kumagai N, Kozaki S, Ushikubi F, Narumiya S. A rho gene product in human platelets: II effects of the ADP-ribosylation by botulinum C3 ADP-ribosyltransferase on platelet aggregation. J Biol Chem 1992; 267: 20921-6.
23. Aepfelbacher M, Vauti F, Weber PC, Glomset JA. Spreading of differentiating human monocytes is associated with a major increase in membrane-bound CDC42. Proc Natl Acad Sci USA 1994; 91: 4263-7.
24. Van der Meulen J, B hullar RP, Chancellor-Maddison KA. Association of a 24-KDa GTP-binding protein, Gn24, with human platelet α-granule membranes. FEBS Lett 1991; 291: 122-6.
25. Witte DP, Harris RE, Jenski LJ, Lampkin BC. Megakaryoblastic leukemia in an infant: establishment of a megakaryocytic tumor cell line in athymic nude mice. Cancer 1986; 58: 238-44.
26. Adachi M, Ryo R, Yoshida A, Sugano W, Yasunaga M, Saigo K, Yamaguchi N, Sato T, Sano K, Kaibuchi K, Takai T. Induction of smg p21/rap1A/Krev-1 p21 gene expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line. Oncogene 1992; 7: 323-9.
27. Pizon V, Lerosey I, Chardin P, Tavitian A. Nucleotide sequence of a human cDNA encoding a ras-related protein (raplB). Nucleic Acid Res 1988; 16: 7719.
28. Nagata K, Nagao S, Nozawa Y. Low Mr GTP-binding proteins in human platelets; cyclic AMP-dependent protein kinase phosphorylates m22KG(I) in membrane but not c21KG in cytosol. Biochem Biophys Res Commun 1989; 160: 235-42.
29. Kawata M, Kikuchi A, Hoshijima M, Yamamoto K, Hashimoto E, Yamamura H, Takai Y. Phosphorylation of smg p21, a ras-like GTP-binding protein, by cyclic AMP-dependent protein kinase in a cell-free system and in response to prostaglandin E1 in intact human platelets. H Biol Chem 1989; 264: 15688-95.
30. Fischer TH, Gatling MC, McCormick F, Duffy CM, White II GC. Incorporation of Rap1B into the platelet cytoskeleton is dependent on thrombin activation and extracellular calcium. J Biol Chem 1994; 269: 17257-61.
31. Bokoch GM. Biology of the Rap proteins, members of the ras superfamily of GTP-binding proteins. Biochem J 1993; 289: 17-24.
32. Madaule P, Axel R. A novel ras-related gene family. Cell 1985; 41: 31-40.
33. Didsbury J, Weber RF, Bokoch GM, Evans T, Snyderman R. rac, a novel ras-related family of proteins that are borulinum toxin substrates. J Biol Chem 1989; 264: 16378-82.
34. Shinjo K, Koland JG, Hart MJ, Narasimhan V, Johnson DJ, Evans T, Cerione R. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein C DC42. Proc Natl Acad Sci USA 1990; 87: 9853-7.
35. Munemitsu S, Innis MA, Clark R, McCormick F, Ullrich A, Polakis P. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene C DC42. Mol Cell Biol 1990; 10: 5977-82.
36. Vincent S, Jeanteur P, Fort P. Growth-regulated expression of rhoG, a new member of the ras homolog gene family. Mol Cell Biol 1992; 12: 3138-48.
37. Drivas GT, Shih A, Coutavas E, Rush MG, D'Eustachio P. Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell Une. Mol Cell Biol 1990; 10: 1793-8.
38. Paterson HF, Self AJ, Garrett MD, Just I, Aktories K, Hall A. Microinjection of p21 rho induces rapid changes in cell morphology. J Cell Biol 1990; 111: 1001-7.
39. Ridley AJ, Paterson HF, Johnson CL, Diekman n D, Hall A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 1992; 70: 401-10.
40. Knaus UG, Heyworth PG, Evans T, Carnutte JT, Bokoch GM. Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac2. Science 1991; 254: 1512-5.
41. Diekmann D, Abo A, Johnston C, Segal AW, Hall A. Interaction of Rac with p67 phox and regulation of phagocytic NADPH oxidase activity. Science 1994; 265: 531-3.
42. Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 1992; 70: 389-99.
43. Nagata K, Suzuki T, Shibagaki Y, Mizumoto K, Okano Y, Kaziro Y, Nozawa Y. Characterization and site-directed mutagenesis of a low Mr GTP-binding protein, ram p25, expressed in Escherichia coli. J Biol Chem 1992; 267: 16000-6.
44. Nagata K, Okano Y, Suzuki T, Nozawa Y. Evidence for the presence of a low Mr GTP-binding protein, ram p25, in human platelet membranes. Platelets 1993; 4: 268-74.
45. Nuoffier C, Balch WE. GTPases: multi-functional molecular switches regulating vesicular traffic. Annu Rev Biochem 1994; 63: 949-90.
46. Matsumoto T, Beach D. Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell 1991; 66: 347-60.
47. Nagata K, Takemasa T, Alam S, Hattori T, Watanabe Y, Nozawa Y. Cloning of cDNAs encoding a cell-cycle-regulatory GTP-binding low-Mr (GBLM) protein, Ran/TC4, from micronucleated Tetrahymena thermophila and amicronucleated Tetrahymena pyriformis. Gene 1994; 144: 123-5.
48. Mindong R, Drivas G, D'Eustachio P, Rush MH. Ran/TC4: a small GTP-binding protein that regulates DNA synthesis. J Cell Biol 1993; 120: 313-23.
49. Moore MS, Blobel G. The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 1993; 365: 661-4.