A simple procedure to determine the biological titer of recombinant retroviral vectors

Gene Therapy

Volumn 4, Issue 2, 1997, Pages 150-155

  Tavoloni, N ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

G418 resistance; Packaging cells; Transduction efficiency; Vector decay; X gal; galactosidase

Indexed keywords

VIRUS VECTOR;

ANIMAL CELL; ARTICLE; GENE TARGETING; GENETIC TRANSDUCTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; RETROVIRUS; TARGET CELL; VIRUS CELL INTERACTION; VIRUS TITRATION;

ANIMALIA; UNIDENTIFIED RETROVIRUS;

EID: 0031058178     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300370     Document Type: Article

References (13)

1. Crystal RG. Transfer of genes to humans: early lessons and obstacles to success. Science 1995; 270: 404-410.
2. Davis HL et al. Plasmid DNA is superior to viral vectors for direct gene transfer into adult mouse skeletal muscle. Hum Gene Ther 1993; 4: 733-740.
3. Miller DG, Edwards RH, Miller AD. Cloning of the cellular receptor for amphotropic murine retroviruses reveals homology to that for gibbon ape leukemia virus. Proc Natl Acad Sci USA 1994; 91: 78-82.
4. van Zeijl M et al. A human amphotropic retrovirus receptor is a second member of the gibbon ape leukemia virus receptor family Proc Natl Acad Sci USA 1994; 91: 1168-1172.
5. Eiden MV, Farrell KB, Wilson CA. Substitution of a single amino acid residue is sufficient to allow the human amphotropic murine leukemia virus receptor to also function as a gibbon ape leukemia virus receptor. J Virol 1996; 70: 1080-1085.
6. Marsh M, Helenius A. Viral entry into animal cells. Adv Viral Res 1989, 36: 107-151.
7. Tavoloni N, Rudenholz A. Variable transduction efficiency of murine leukemia retroviral vectors on mammalian cells: role of cellular glycosylation. Virology (in press)
8. Klement V, Rowe WP, Hartley JW, Pugh WE. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc Natl Acad Sci USA 1969; 63: 753-758.
9. Morgan JR et al. Retrovirus infection: effect of time and target cell number. J Virol 1995; 69: 6994-7000.
10. Paul RW et al. Increased viral titer through concentration of viral harvests from retroviral packaging lines. Hum Gene Ther 1993; 4: 609-615.
11. Russel DW, Berger MS, Miller DA. The effects of human serum and cerebrospinal fluid on retroviral vectors and packaging cell lines. Hum Gene Ther 1995; 6: 635-641.
12. Le Doux JM, Morgan JR, Snow RG, Yarmush ML. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J Virol 1996; 70: 6468-6473.
13. Cepko C. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. In: Conn PM (ed). Methods in Neurosciences, Vol. 1. Academic Press: New York, 1989, pp 367-392.