The expression of bacterial nitroreductase in transgenic mice results in specific cell killing by the prodrug CB1954

Gene Therapy

Volumn 4, Issue 2, 1997, Pages 93-100

  Drabek, D ^a   Guy, J ^a   Craig, R ^b   Grosveld, F ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b KEELE UNIVERSITY   (United Kingdom)

Author keywords

CB1954; Locus control region; Nitroreductase; Transgene

Indexed keywords

5 (1 AZIRIDINYL) 2,4 DINITROBENZAMIDE; BACTERIAL ENZYME; NITROREDUCTASE; PRODRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENE; CANCER; CELL KILLING; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FIBROBLAST; GENE THERAPY; INTRAPERITONEAL DRUG ADMINISTRATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN; T LYMPHOCYTE; THYMUS; TRANSGENIC MOUSE;

ANIMALIA; BACTERIA (MICROORGANISMS); CUCUMBER MOSAIC VIRUS; ESCHERICHIA; ESCHERICHIA COLI B; EUKARYOTA; MUS MUSCULUS;

EID: 0031058115     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300366     Document Type: Article

References (31)

1. Khan A, Ross WCJ. Tumour growth inhibitory nitrophenylaziridines and related compounds: structure-activity relationships. Chem Biol Interact 1969; 1: 27-47.
2. Cobb LM. Toxicity of the selective antitumour agent 5-aziridino-2,4 dimtrobenzamide in rat. Toxicol Appl Pharamcol 1970; 17: 231-238.
3. Knox RJ, Friedlos F, Marchbank T, Roberts JJ. Bioactivation of CB1954 reaction of the active 4-hydroxylamino derivative with thioesters to form the ultimate DNA-DXA interstrand crosslinking species. Biochem Pharmacol 1991; 42: 1691-1697.
4. Boland MP, Knox RJ, Roberts JJ. The differences in kinetics of rat and human DT diaphorase result in a differential sensitivity of derived cell lines to CB1954 (5-(aziridin-1yl)-2,4-dinitrobenzamide). Biochem Pharmacol 1991; 41: 867-875.
5. Venitt S. The differential response of resistant and sensitive strains of Escherichia coli to the cytotoxic effects of 5-aziridino-2,4-dinitrobenzamide (CB 1954). Chem Biol Interac 1971; 3: 177-191.
6. Venitt S, Crofton-Sleigh C. The toxicity and mutagenicity of the anti-tumour drug 5-aziridino-2,4-dinitrobenzamide (CB1954) is greatly reduced in a nitroreductase deficient strain of E. coli. Mutagenesis 1987; 2: 371-381.
7. Anlezark GM et al. The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)-I. Biochem Pharmacol 1992; 44: 2289-2295.
8. Knox RJ et al. The bioactivation of 5-(aziridin-l-yl)-2,4-dinitrobenzamide (CB1954)-II. Biochem Pharmacol 1992; 44: 2297-2301.
9. Bagshawe KD. Antibody-directed enzyme prodrug therapy (ADEPT). Biochem Soc Transac 1990; 18: 750-751.
10. Michael NP, Brehm JK, Anlezark GM, Minton NP. Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its overexpression in Escherichia coli K12. FEMS Microbiol Lett 1994; 124: 195-202.
11. Grosveld F, Blom van Assendelft G, Greaves DR, Kollias G. Position-independent, high-level expression of the human β-globin gene in transgenic mice. Cell 1987; 51: 975-985.
12. Greaves DR, Wilson FD, Lang G, Kioussis D. Human CD2 3′-flanking sequences confer high-level, T cell-specific, position-independent gene expression in transgenic mice. Cell 1989; 56: 979-986.
13. Bonifer C, Vidal M, Grosveld F, Sippel AE. Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice. EMBO J 1990; 9: 2843-2848.
14. Friend WC et al. Cell-specific expression of high levels of human SlOOjS in transgenic mouse brain is dependent on gene dosage. J Neurosci 1992; 12: 4337-4346.
15. Carson S, Wiles MV. Far upstream regions of class II MHC Ea are necessary for position-independent, copy-dependent expression of Ea transgene. Nucleic Acids Res 1993; 21: 2065-2072.
16. Walters MC et al. Transcriptional enhancers act in ris to suppress position-effect variegation. Genes Dev 1996; 10: 185-195.
17. Workman P et al. CB1954 revisited. I: disposition kinetics and metabolism. Cancer Chemother Pharmacol 1986; 16: 1-8.
18. Workman P et al. CB1954 revisited. II: toxicity and antitumour activity. Cancer Chemother Pharmacol 1986; 16: 9-14.
19. Sheard CE, Double JA, Berenbaum MC. The sensitivity to chemotherapeutic agents of a rat tumour grown in immunosuppressed mice. Br J Cancer 1971; 25: 838-844.
20. Friedlos F, Quinn J, Knox RJ, Roberts JJ. The properties of total adducts and interstrand crosslinks in the DNA of cells treated with CB 1954. Biochem Pharmacol 1992; 43: 1249-1254.
21. Knox RJ, Friedlos F, Jarman M, Roberts JJ. A new cytotoxic, DNA interstrand crosslinking agent, 5-(aziridin-1-yl)-4-hydroxylamino-2-nitrobenzamide, is formed from 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954) by a nitroreductase enzyme in Walker carcinoma cells. Biochem Pharmacol 1988; 37: 4661-4669.
22. Bridgewater JA et al. Expression of the bacterial nitroreductase enzyme in mammalian cells renders them selectively sensitive to killing by the prodrug CB1954 Eur J Cancer 1995; 31: 2362-2370.
23. Lang G et al. Deletion analysis of the human CD2 gene locus control region in transgenic mice. Nucleic Acids Res 1991; 19: 5851-5856.
24. Zhurnabekov T, Corbella P, Tolaini M, Kioussis D. Improved version of a human CD2 minigene based vector for T cell-specific expression in transgenic mice. J Immunol Meth 1995; 185: 133-140.
25. Hickman JA. Apoptosis induced by anticancer drugs. Cancer Metas Rev 1992; 11: 121-139.
26. Knox RJ, Friedlos F, Boland MP. The bioactivation of CB1954 and its use as a prodrug in antibody-directed enzyme prodrug therapy (ADEPT). Cancer Metas Rev 1993; 12: 195-212.
27. De Bruijn MFTR et al. Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens. Eur J Immunol 1994; 24: 2279-2284.
28. Antoniou M. Induction of erythroid-specific expression in marine erythroleukemia (MED cell lines. In: Murray EJ (ed). Methods in Molecular Biology: Gene Transfer and Expression Protocols. The Humana Press: Clifton, NJ, 1991, pp 421-434.
29. Ellis J et al. A dominant chromatin-opening activity in 5′ hyper- sensitive site 3 of the human β-globin locus control region. EMBO J 1996; 15: 562-568.
30. Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503-517.
31. Slieker WAT, de Rijk-de Bruijn MFTR, Leenen PJM, Ewijk W. ER-MP12 antigen, a new surface marker on mouse bone marrow cells with thymus-repopulating ability. I. Intrathymic repopulating ability of ER-MP12-positive bone marrow cells. Int Immunol 1993; 5: 1093-1098.