gpt-gus Fusion gene for selection and marker in recombinant poxviruses

BioTechniques

Volumn 22, Issue 2, 1997, Pages 276-278

  Cao, JingXin ^a   Upton, Chris ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA DETERMINATION; GENE EXPRESSION; GENE FUSION; GENE ISOLATION; MARKER GENE; POXVIRUS; PROTEIN ANALYSIS; PROTEIN PURIFICATION; RECOMBINANT GENE;

POXVIRIDAE;

EID: 0031057606     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm17     Document Type: Article

References (11)

1. Carroll, M.W. and B. Moss. 1995. E. coli β-glucuronidase (GUS) as a marker for recombinant vaccinia viruses. BioTechniques 19:352-356.
2. Chakrabarti, S., K. Brechling and B. Moss. 1985. Vaccinia virus expression vector: co-expression of β-galactosidase gene provides visual screening of recombinant virus plaques. Mol. Cell. Biol. 5:3403-3409.
3. Earl, P.L. and B. Moss. 1995. Expression of proteins in mammalian cells using vaccinia virus expression system. Chapter 16, Units 15-18. Current Protocols in Molecular Biology. John Wiley & Sons, New York.
4. Falkner, F.G. and B. Moss. 1988. Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J. Virol. 62:1849-1854.
5. Franke, C.A., C.M. Rice, J.H. Strauss and D.E. Hruby. 1985. Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants. Mol. Cell. Biol. 5:1918-1924.
6. Gallie, D.R., V. Walbot and, J.N. Feder. 1992. GUS as a useful reporter gene in animal cells. p. 181-188. In S.R. Gallagher (Ed.), GUS Protocols: Using GUS Gene as a Reporter of Gene Expression. Academic Press, San Diego.
7. Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. 5:387-405.
8. Jefferson, R.A., M. Klass, N. Wolf and D. Hirsh. 1987. Expression of chimeric genes in Caenorhabiditis elegans. J. Mol. Biol. 193:41-46.
9. Mulligan, R. and P. Berg. 1981. Selection for animal cells that express the E. coli gene coding for xanthine-guanine phophoribosyl transferase. Proc. Natl. Acad. Sci. USA 78:2072-2076.
10. Panicali, D., A. Grzelecki and C. Huang. 1986. Vaccinia virus vectors utilizing the β-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression. Gene 47:193-199.
11. Tartaglia, J., S. Pincus and E. Paoletti. 1990. Poxvirus-based vectors as vaccine candidates. Crit. Rev. Immunol. 10:13-30.