Analysis of protein structure in intact cells: Crosslinking in vivo between introduced cysteines in the transmembrane domain of a bacterial chemoreceptor

Protein Science

Volumn 6, Issue 2, 1997, Pages 315-322

  Hughson, Andrew G ^a   Lee, Geoffrey F ^a,b   Hazelbauer, Gerald L ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b GENENTECH INC   (United States)

Author keywords

bacterial chemotaxis; helical bundles; transmembrane helices; transmembrane receptors

Indexed keywords

1,10 PHENANTHROLINE COPPER; CYSTEINE;

ARTICLE; CHEMORECEPTOR; ESCHERICHIA COLI; MEMBRANE BINDING; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

EID: 0031056669     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060206     Document Type: Article

References (34)

1. Bourret RA, Borkovich KA, Simon MI. 1991. Signal transaction pathways involving protein phosphorylation in prokaryotes. Annu Rev Biochem 60:401-441.
2. Bowie JU, Pakula AA, Simon MI. 1995. The three-dimensional structure of the aspartate receptor from Escherichia coli. Acta Cryst D51:145-154.
3. Burrows GG, Newcomer ME, Hazelbauer GL. 1989. Purification of receptor protein Trg by exploiting a property common to chemotactic transducers of Escherichia coli. J Biol Chem 264:17309-17315.
4. Carreaga CL, Falke JJ. 1992. Thermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping. J Mol Biol 226:1219-1235.
5. Chervitz SA, Falke JJ. 1995. Lock on/off disulfides identify the transmembrane signaling helix of the aspartate receptor. J Biol Chem 270:24043-24053.
6. Chervitz SA, Lin CM, Falke JJ. 1995. Transmembrane signaling by the aspartate receptor: Engineered disulfides reveal static regions of the subunit interface. Biochemistry 54:9722-9733.
7. Duché D, Parker MW, González-Manas JM, Pattus F, Baty D. 1994. Uncoupled steps of the colicin A pore formation demonstrated by disulfide bond engineering. J Biol Chem 269:6332-6339.
8. Falke JJ, Koshland DE Jr. 1987. Global flexibility in a sensory receptor: A site-directed crosslinking approach. Science 237:1597-1600.
9. Falke JJ, Dernburg AF, Sternberg DA, Zalkin N, Milligan DL, Koshland DE Jr. 1988. Structure of a bacterial sensory receptor. A site-directed sulfhydryl study. J Biol Chem 265:14850-14858.
10. Francis G, Brennan L, Ferenci T. 1991. Affinity-chromatographic purification of sixteen cysteine-substituted maltoporin variants: Thiol reactivity and cross-linking in an outer membrane protein of Escherichia coli. Biochim Biophys Acta 1067:89-96.
11. Gegner JA, Graham DR, Roth AF, Dahlquist FW. 1992. Assembly of an MCP receptor, CheW, and kinase CheA complex in the bacterial chemotaxis signal transduction pathway. Cell 70:975-982.
12. Hazelbauer GL, Mesibov RE, Adler J. 1969. Escherichia coli mutants defective in chemotaxis towards specific chemicals. Proc Natl Acad Sci USA 64:1300-1307.
13. Hazelbauer GL. 1992. Bacterial chemoreceptors. Curr Opinion Struct Biol 2:505-510.
14. Iida A, Harayama S, Iino T, Hazelbauer GL. 1984. Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12. J Bacteriol 158:674-682.
15. Karnik SS, Khorana HG 1990. Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187. J Biol Chem 265:17520-17524.
16. Lee GF, Burrows GG, Lebert MR, Dutton DP, Hazelbauer GL. 1994. Deducing the organization of a transmembrane domain by disulfide crosslinking: The bacterial chemoreceptor Trg. J Biol Chem 269:29920-29927.
17. Lee GF, Dutton DP, Hazelbauer GL. 1995a. Identification of functionally important helical faces on transmembrane segments by scanning mutagenesis. Proc Natl Acad Sci USA 92:5416-5420.
18. Lee GF, Lebert MR, Lilly AL, Hazelbauer GL. 1995b. Transmembrane signaling characterized in bacterial chemoreceptors by using sulfhydryl crosslinking in vivo. Proc Natl Acad Sci USA 92:3391-3395.
19. Lee MH, Ohta T, Walker GC. 1994. A monocysteine approach for probing the structure and interactions of the UmuD protein. J Bacteriol 176:4825-4837.
20. Lynch BA, Koshland DE Jr. 1991. Disulfide crosslinking studies of the transmembrane regions of the aspartate sensory receptor of Escherichia coli. Proc Natl Acad Sci USA 88:10402-10406.
21. Maruyama IN, Mikawa YG, Maruyama HI. 1995. A model for transmembrane signaling by the aspartate receptor based on random-cassette mutagenesis and site-directed disulfide crosslinking. J Mol Biol 253:530-546.
22. Milburn MV, Privé GG, Milligan DL, Scott WG, Yeh J, Jancarik J, Koshland DE Jr, Kim S-H. 1991. Three-dimensional structures of the ligand-binding domain of the bacterial aspartate receptor with and without a ligand. Science 254:1342-1347.
23. Milligan DL, Koshland DE Jr. 1988. Site-directed crosslinking. Establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis. J Biol Chem 263:6268-6275.
24. Morgan DG, Baumgartner JW, Hazelbauer GL. 1993. Proteins antigenically related to methyl-accepting chemotaxis proteins of Escherichia coli detected in a wide range of bacterial species. J Bacteriol 175:133-140.
25. Ordal GW, Adler J. 1974. Isolation and complementation of mutants in galactose taxis and transport. J Bacteriol 117:509-516.
26. Pakula AA, Simon MI. 1991. Random cysteine disulfide crosslinking in the analysis of protein structure. Methods 3:175-181.
27. Pakula AA, Simon MI. 1992. Determination of transmembrane protein structure by disulfide crosslinking: the Escherichia coli Tar receptor. Proc Natl Acad Sci USA 89:4144-4148.
28. Park C, Hazelbauer GL. 1986. Mutations specifically affecting ligand interaction of the Trg chemosensory transducer. J Bacteriol 167:101-109.
29. Schuster SC, Swanson RV, Alex LA, Bourret RB, Simon MI. 1993. Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance. Nature 365:343-347.
30. Scott WG, Stoddard BL. 1994. Transmembrane signaling and the aspartate receptor. Structure 2:877-887.
31. Stoddard BL, Bui JD, Koshland DE Jr. 1992. Structure and dynamics of transmembrane signaling by the Escherichia coli aspartate receptor. Biochemistry 31:11978-11983.
32. Whitley P, Nilsson L, von Heijne G. 1993. Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry 32:8534-8539.
33. Yeh JI, Biemann HP, Pandit J, Koshland DE Jr, Kim S-H. 1993. The three-dimensional structure of the ligand-binding domain of a wild-type bacterial chemotaxis receptor. Structural comparison to the crosslinked mutant forms and conformational changes upon ligand binding. J Biol Chem 268:9787-9792.
34. + transport and ATP synthesis in the Escherichia coli ATP synthase. Cys-Cys crosslinking of F1 subunit epsilon to the polar loop of F0 subunit c. J Biol Chem 270:24609-24614.