Immunohistochemical double staining of microwave enhanced and nonenhanced nuclear and cytoplasmic antigens

Biotechnic and Histochemistry

Volumn 72, Issue 1, 1997, Pages 10-15

  Bohle, Rainer M ^a   Bonczkowitz, Matthias ^a   Altmannsberger, Hans M ^b   Schulz, Andreas ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b KRANKENHAUS NORDWEST   (Germany)

Author keywords

antigen retrieval; double staining; formalin fixation; immunohistochemistry; microwave; paraffin embedment

Indexed keywords

ANTIGEN ANTIBODY COMPLEX; ANTIGEN DETECTION; ARTICLE; CELL COMPARTMENTALIZATION; CELL POPULATION; CELL PROLIFERATION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MICROWAVE RADIATION; TISSUE SECTION; TUMOR DIFFERENTIATION;

EID: 0031055668     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299709082205     Document Type: Article

References (23)

1. Allred, D. C., Clark, G. M., Mokina, R., Tandon, A. K., Schnitt, S. J., Gilchrist, K. W., Osborne, C. K., Tormey, D. C. and McGuire, W. L. 1992. Overexpression of HER-2/neu and its relationship with other prognostic factor changes during the progression of in situ to invasive breast cancer. Hum. Pathol. 23: 974-979.
2. Bell, P. B., Jr., Rundquist, I., Svensson, I. and Collins, V. P. 1987. Formaldehyde sensitivity of GFAP epitope, removed by the extraction of the cytoskeleton with high salt. J. Histochem. Cytochem. 35: 1375-1380.
3. Catoretti, G., Pileri, S., Parravicini, C., Becker, M. H. G., Poggi, S., Gerdes, J. and Rilke, F. 1993. Antigen unmasking of formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171: 83-98.
4. Cordell, J. L., Falini, B., Erber, W. N., Ghosh, A. K., Abdulaziz, Z., Macdonald, S., Pulford, K. A. F., Stein, H. and Mason, D. Y. 1984. Immunoenzymatic labeling of monoclonal antibodies using immunocomplexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32: 219-229.
5. Falini, B., Abdulaziz, Z., Gerdes, J., Canino, S., Ciani, C., Cordell, J. L., Knight, P. M., Stein, H., Grignani, F., Martinelli, M. F. and Mason, D. Y. 1986. Description of a sequential staining procedure for double immunoenzymatic staining of pairs of antigens using monoclonal antibodies. J. Immunol. Methods 93: 265-273.
6. Gerdes, J., Becker, M. H. G., Key, G. and Catoretti, G. 1992. Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol. 168: 85-87.
7. Giorno, R. 1984. A comparison of two immunoperoxidase staining methods based on the avidin-biotin interaction. Diagn. Immunol. 2: 161-166.
8. Hiort, O., Kwan, P. W. L. and DeLellis, R. A. 1988. Immunohistochemistry of estrogen receptor protein in paraffin sections. Am. J. Clin. Pathol. 90: 559-563.
9. Malik, N. J. and Daymon, M. E. 1982. Improved double immunoenzyme labeling using alkaline phosphatase and horseradish peroxidase. J. Clin. Pathol. 35: 1092-1094.
10. Mason, D. Y. 1983. Double immunoenzymatic labelling. In: Immunocytochemistry. Practical Applications in Pathology and Biology. Polak, M. and Van Norden, S., Eds. Wright PSG, Boston, Bristol, London, pp. 113-128.
11. Nakane, I. K. 1968. Simultaneous localization of multiple tissue antigens using the peroxidase-labelled antibody method: a study on pituitary glands of the rat. J. Histochem. Cytochem. 16: 557-558.
12. Perrot-Applanat, M., Groyer-Picard, M. T., Lorenzo, F., Jolivet, A., Vu-Hai, M. T., Pallud, C., Spyratos, F. and Milgrom, E. 1987. Immunocytochemical study with monoclonal antibodies to progesterone receptor in human breast tumours. Cancer Res. 47: 2652-2661.
13. Rindi, G., Buffa, R., Sessa, F., Tortora, O. and Solcia, E. 1986. Chromogranin A, B and C immunoreactivities of mammalian endocrine cells. Histochemistry 85: 19-28.
14. Sadi, M. V., Walsh, P. C. and Barrack, E. R. 1991. Immunohistochemical study of androgen receptors in metastatic prostate cancer, comparison of receptor content and response to hormonal therapy. Cancer 67: 3057-3064.
15. Shi, S. R., Key, M. E. and Kalra, K. L. 1991. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue. J. Histochem. Cytochem. 39: 741-748.
16. Shi, S. R., Cote, C., Kalra, K. L., Taylor, C. R. and Tandon, A. K. 1992. A technique for retrieving antigens in formal in-fixed, routinely acid decalcified, colloidin-embedded human temporal bone sections for immunohistochemistry. J. Histochem. Cytochem. 40: 787-792.
17. Shi, S. R., Chaiwun, B., Young, L., Cote, R. J. and Taylor, C. R. 1993. Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J. Histochem. Cytochem. 41: 1599-1604.
18. Sternberger, L. A. and Joseph, S. A. 1979. The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal. J. Histochem. Cytochem. 27: 1424-1429.
19. Valnes, K. and Brandtzaeg, P. 1984. Paired indirect immunoenzyme staining with primary antibodies from the same species. Application of horse-radish peroxidase and alkaline phosphatase as sequential labels. Histochem. J. 16: 477-487.
20. Van der Loos, C. M., Das, P. K., van den Oord, J. J. and Houthoff, H. J. 1989. Multiple immunoenzyme staining techniques. Use of fluoresceinated, biotinlylated and unlabeled monoclonal antibodies. J. Immunol. Methods 117: 45-52.
21. Van der Loos, C. M., Becker, A. E. and van den Oord, J. J. 1993. Practical suggestions for successful immunoenzyme double-staining experiments. Histochem. J. 25: 1-13.
22. Wilbur, D. C., Willis, J., Mooney, R. A., Fallon, M. A., Moynes, R. and di Sant'Agnese, P. A. 1992. Estrogen and progesterone receptor detection in archival, formalin-fixed paraffin-embedded tissue from breast carcinoma: a comparison of immunohistochemistry with dextran coated charcoal assay. Mod. Pathol. 5: 79-84.
23. Wolf, H. K. and Dittrich, K. L. 1992. Detection of proliferation nuclear cell antigen in diagnostic histopathology. J. Histochem. Cytochem. 40: 1269-1273.