Use of a fluorescent probe to assess the activities of candidate agents against intracellular forms of Encephalitozoon microsporidia

Antimicrobial Agents and Chemotherapy

Volumn 41, Issue 2, 1997, Pages 337-344

  Leitch, Gordon J ^a   Scanlon, Mary ^a   Shaw, Andrew ^a   Visvesvara, Govinda S ^b   Wallace, Sara ^b  

^a MOREHOUSE SCHOOL OF MEDICINE   (United States)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBENDAZOLE; ANTIPROTOZOAL AGENT; CALCEIN; FLUORESCENT DYE; FUMAGILLIN; HYDROGEN PEROXIDE; ITRACONAZOLE; METRONIDAZOLE; VERAPAMIL;

ANIMAL CELL; ANTIPROTOZOAL ACTIVITY; ARTICLE; CELL VACUOLE; CELL VIABILITY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DRUG SCREENING; FLUORESCENCE; HOST CELL; MICROSPORA; MONKEY; NONHUMAN; PRIORITY JOURNAL;

EID: 0031054654     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.41.2.337     Document Type: Article

References (33)

1. Beauvais, B., C. Sarfati, S. Challier, and F. Derouin. 1994. In vitro model to assess effect of antimicrobial agents on Encephalitozoon cuniculi. Antimicrob. Agents Chemother. 38:2440-2448.
2. Blanshard, C., D. S. Ellis, S. P. Dowell, G. Tovey, and B. G. Gazzard. 1993. Electron microscopic changes in Enterocytozoon bieneusi following treatment with albendazole. J. Clin. Pathol. 46:898-902.
3. Cali, A., D. P. Kotler, and J. M. Orenstein. 1993. Septata intestinalis, N.G., N.Sp., an intestinal microsporidian associated with chronic diarrhea and dissemination in AIDS patients. J. Eukaryot. Microbiol. 40:101-112.
4. Canning, E. U., and J. Lom. 1986. The microsporidia of vertebrates. Academic Press, London, England.
5. Curray, A., and E. U. Canning. 1993. Human microsporidiosis. J. Infect. 27:229-236.
6. De Beule, K. 1996. Itraconazole: pharmacology, clinical experience and future development. Int. J. Antimicrob. Agents 6:175-181.
7. DeGroote, M. A., G. S. Visvesvara, M. L. Wilson, N. J. Pieniazek, S. B. Slomeda, A. J. Da Silva, G. J. Leitch, R. T. Bryan, and R. Reeves. 1995. Polymerase chain reaction and culture confirmation of disseminated Encephalitozoon cuniculi in a patient with AIDS. J. Infect. Dis. 171:1375-1378.
8. Desportes, I., Y. Le Charpentier, A. Galian, F. Bernard, B. Cochand-Priollet, A. Lavergne, P. Ravisse, and R. Modigliani. 1985. Occurrence of a new microsporidian: Enterocytozoon bieneusi n.g.; n. sp., in the enterocytes of a human patient with AIDS. J. Protozool. 30:250-254.
9. Didier, E. S., P. J. Didier, D. N. Frieberg, S. M. Stenson, J. M. Orenstein, R. W. Yee, F. O. Tio, R. M. Davis, C. Vossbrink, N. Millichamp, and J. A. Shadduck. 1991. Isolation and characterization of a new species of microsporidian, Encephalitozoon hellem (n. sp.), from three AIDS patients with keratoconjunctivitis. J. Infect. Dis. 163:617-621.
10. Didier, E. S., P. W. Varner, P. J. Didier, A. M. Aldras, N. J. Millichamp, M. Murphy-Corb, R. Bohm, and J. A. Shadduck. 1994. Experimental microsporidiosis in immunocompetent and immunodeficient mice and monkeys. Folia Parasitol. (Ceske Budejovice) 41:1-11.
11. Dieterich, D. T., A. L. Lew, D. P. Kotler, M. A. Poles, and J. M. Orenstein. 1994. Treatment with albendazole for intestinal disease due to Enterocytozoon bieneusi in patients with AIDS. J. Infect. Dis. 169:178-182.
12. Dore, G. J., D. J. Marriott, M. C. Hing, J. L. Harkness, and A. S. Field. 1995. Disseminated microsporidiosis due to Septata intestinalis in nine patients infected with the human immunodeficiency virus: response to therapy with albendazole. Clin. Infect. Dis. 21:70-76.
13. Eeftinck Schattenkerk, J. K. M., T. van Gool, R. J. van Ketel, J. F. W. M. Bartelsman, K. L. Kuiken, W. J. Terpstra, and P. Reiss. 1991. Clinical significance of small intestinal microsporidiosis in HIV-1 infected individuals. Lancet 337:895-898.
14. Garvey, M. J., P. G. Ambrose, and J. L. Ulmer. 1995. Topical fumagillin in the treatment of microsporidial keratoconjunctivitis in AIDS. Ann. Pharmacother. 29:872-874.
15. Hartskeerl, R. A., T. van Gool, A. R. J. Schuitema, E. S. Didier, and W. J. Terpstra. 1995. Genetic and immunological characterization of the microsporidian, Septata intestinalis Cali, Kotler, and Orenstein, 1993; reclassification to Encephalitozoon intestinalis. Parasitology 110:277-285.
16. He, Q., G. J. Leitch, G. S. Visvesvara, and S. Wallace. 1996. Effects of nifedipine, metronidazole, and nitric oxide donors on spore germination and cell culture infection of the microsporidia Encephalitozoon hellem and Encephalitozoon intestinalis. Antimicrob. Agents Chemother. 40:179-185.
17. Homolya, L., Z. Hollo, U. A. Germann, I. Pastan, M. M. Gottesman, and B. Sarkadi. 1993. Fluorescent cellular indicators are extruded by the multidrug resistance protein. J. Biol. Chem. 268:21493-21496.
18. Kurosawa, M., M. Okabe, N. Hara, K. Kawamura, S. Suzuki, K. Sakurada, and M. Asaka. 1996. Reversal effect of itraconazole on adriamycin and etoposide resistance in human leukemia cells. Ann. Hematol. 72:17-21.
19. Leitch, G. J., Q. He, S. Wallace, and G. S. Visvesvara. 1993. Inhibition of spore polar filament extrusion of the microsporidium, Encephalitozoon hellem, isolated from an AIDS patient. J. Eukaryot. Microbiol. 40:711-717.
20. Leitch, G. J., M. Scanlon, G. S. Visvesvara, and S. Wallace. 1995. Calcium and hydrogen ion concentrations in the parasitophorous vacuoles of epithelial cells infected with the microsporidian Encephalitozoon hellem. J. Eukaryot. Microbiol. 42:445-451.
21. Leitch, G. J., G. S. Visvesvara, and Q. He. 1993. Inhibition of microsporidian spore germination. Parasitol. Today 9:422-424.
22. Liu, T. P., and G. R. Myrick. 1989. Deformities in the spore of Nosema apis as induced by itraconazole. Parasitol. Res. 75:498-502.
23. Orenstein, J. M. 1991. Microsporidiosis in the acquired immunodeficiency syndrome. J. Parasitol. 77:843-864.
24. 2+ influx. J. Cell Biol. 100:1834-1838.
25. Poole, C. A., N. H. Brookes, and G. M. Glover. 1993. Keratocyte networks visualized in the living cornea using vital dyes. J. Cell Sci. 106:685-691.
26. Shadduck, J. A. 1980. Effect of fumagillin on in vitro multiplication of Encephalitozoon cuniculi. J. Protozool. 27:202-208.
27. Shadduck, J. A. 1989. Human microsporidiosis and AIDS. Rev. Infect. Dis. 11:203-207.
28. Steinberg, T. H., A. S. Newman, J. A. Swanson, and S. C. Silverstein. 1987. Macrophages possess probenecid-inhibitable organic anion transporters that remove fluorescent dyes from the cytoplasmic matrix. J. Cell Biol. 105:2695-2702.
29. Visvesvara, G. S., A. J. da Silva, G. P. Croppo, N. J. Pieniazek, G. J. Leitch, D. Ferguson, H. de Moura, S. Wallace, S. B. Slemenda, I. Tyrrell, D. F. Moore, and J. Meador. 1995. In vitro culture and serologic and molecular identification of Septata intestinalis isolated from urine of a patient with AIDS. J. Clin. Microbiol. 33:930-936.
30. Visvesvara, G. S., G. J. Leitch, H. de Moura, S. Wallace, R. Weber, and R. T. Bryan. 1991. Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS. J. Protozool. 38:S105-S111.
31. Visvesvara, G. S., G. J. Leitch, N. J. Pieniazek, A. J. Da Silva, S. Wallace, S. B. Slemenda, R. Weber, D. A. Schwartz, L. Gorelkin, C. M. Wilcox, and R. T. Bryan. 1995. Short-term in vitro culture and molecular analysis of the microsporidian, Enterocytozoon bieneusi. J. Eukaryot. Microbiol. 42:506-510.
32. Wall, D. M., X. F. Hu, J. R. Zalcberg, and J. D. Parkin. 1991. Rapid functional assay for multidrug resistance in human tumor cell lines using the fluorescent indicator Fluo-3. J. Natl. Cancer Inst. 83:206-207.
33. Weber, R., and R. T. Bryan. 1994. Microsporidial infections in immunodeficient and immunocompetent patients. Clin. Infect. Dis. 19:517-521.