Comparison between cytomegalovirus promoter and elongation factor-1α promoter-driven constructs in the establishment of cell lines expressing hepatitis C virus core protein

Journal of Virological Methods

Volumn 64, Issue 1, 1997, Pages 73-80

  Tokushige, Katsutoshi ^a   Moradpour, Darius ^a   Wakita, Takaji ^a   Geissler, Michael ^a   Hayashi, Naoaki ^b   Wands, Jack R ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

elongation factor 1 promoter; HCV nucleocapsid protein stable cell lines; human hepatocellular carcinoma

Indexed keywords

CORE PROTEIN; ELONGATION FACTOR 1ALPHA;

ARTICLE; CELL LINE; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOTOXICITY; GENE EXPRESSION REGULATION; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; LIVER CARCINOMA; MYELOMA; PRIORITY JOURNAL; PROMOTER REGION; VIRUS CORE;

ANIMALS; CYTOMEGALOVIRUS; GENE EXPRESSION REGULATION; HEPACIVIRUS; HEPATITIS B SURFACE ANTIGENS; HUMANS; MICE; PEPTIDE ELONGATION FACTOR 1; PEPTIDE ELONGATION FACTORS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TUMOR CELLS, CULTURED; VIRAL CORE PROTEINS;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; HEPATITIS B VIRUS; HEPATITIS C VIRUS; MURINAE;

EID: 0031053683     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(96)02143-X     Document Type: Article

References (26)

1. Aden, D.P., Fogel, A., Plotkin, S., Damjanov, I. and Knowles, B.B. (1979) Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282, 615-616.
2. Alfredo, A., Morsica, G., Chemello, L., Cavalletto, D., Noventa, F., Pontisso, P. and Roul, A. (1990) Hepatitis C viremia and liver disease in symptom-free individuals with anti-HCV. Lancet 340, 697-698.
3. Alter, H.J., Purcell, R.H., Shihi, J., Melpolder, C., Houghton, M. and Choo, Q.L. (1989) Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N. Engl. J. Med. 321, 1494-1500.
4. Battegay, M., Fikes, J., Bisceglie, M.D., Wentworth, P.G., Sette, A., Celis, E., Ching, W.M., Grakoui, A., Rice, C.M., Kurokohchi, K., Berzofsky, J.A. and Akatsuka, T. (1995) Patients with chronic hepatitis C have circulating cytotoxic T cells which recognize hepatitis C virus-encoded peptides binding to HLA-A2.1 molecules. J. Virol. 69, 2462-2470.
5. Cerny, C.A., McHutchinson, J.G., Pasquinelli, C., Brown, M.E., Brothers, M.A., Grabscheid, B., Fowler, P., Houghton, M. and Chisari, F.V. (1995) Cytotoxic T lymphocyte response to hepatitis C virus-derived peptides containing the HLA A2.1 binding motif. J. Clin. Invest. 95, 521-530.
6. Choo, Q.L., Kuo, G., Weiner, A.J., Overby, L.R., Bradley, D.W. and Houghton, M. (1989) Isolation of a cDNA clone derived from a blood-born non-A, non-B viral hepatitis genome. Science 244, 359-362.
7. Choo, Q.L., Richman, K.H., Berger, K., Lee, C., Dong, C., Gallegos, C., Coit, D., Medina-Selby, A., Barr, P.J., Weiner, A.J., Bradley, D.W., Kuo, G. and Houghton, M. (1991) Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA. 88, 2451-2455.
8. Grakoui, A., Wychowski, C., Lin, C., Feinstone, S.M. and Rice, C. (1993) Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67, 1385-1395.
9. Kato, N., Hijikata, M., Ootsuka, Y., Nakagawa, M., Ohkoshi, S., Sugimura, T. and Shimotohno, K. (1990) Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc. Natl. Acad. Sci. USA. 87, 9524-9528.
10. Kim, D.W., Uetsuki, T., Kaziro, Y., Yamaguchi, N. and Sugano, S. (1990) Use of the human elongation factor 1 a promoter as a versatile and efficient expression system. Gene 91, 217-223.
11. Kim, D.W., Harada, T., Saito, I. and Miyamura, T. (1993) An efficient expression vector for stable expression in human liver cells. Gene 134, 307-308.
12. Kim, D.W., Suzuki, R., Harada, T., Saito, I. and Miyamura, T. (1994) Trans-suppression of gene expression by hepatitis C viral core protein. Jpn. J. Med. Sci. Biol. 47, 211-220.
13. Kita, H., Moriyama, T., Kaneko, T., Harase, I., Nomura, M., Miura, H., Nakamura, I., Yazaki, Y. and Imawari, M. (1993) HLA B44 restricted cytotoxic T lymphocytes recognizing an epitope on hepatitis C virus nucleocapsid protein. Hepatology, 18, 1039-1044.
14. Koziel, M.J., Dudley, D., Wong, J.T., Diensytg, J., Houghton, M., Ralston, R. and Walker, B.D. (1992) Intrahepatic cytotoxic T lymphocytes specific for hepatitis C virus in persons with chronic hepatitis. J. Immunol. 149, 3339-3344.
15. Koziel, M.J., Dudley, D., Afdhal, N., Choo, Q.L., Houghton, M., Ralston, R. and Walker, B.D. (1993) Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes recognize an epitope in the core and envelope protein of HCV. J. Virol. 67, 7522-7532.
16. Moradpour, D., Wakita, T., Tokushige, K., Carlson, R.I., Krawczynski, K. and Wands, J.R. (1996) Characterization of three novel monoclonal antibodies to hepatitis C virus core protein. J. Med. Virol. 48, 234-241.
17. Moradpour, D., Englert, C., Wakita, T. and Wands, J.R. (1996) Characterization of cell lines allowing tightly regulated expression of hepatitis C virus core protein. Virology, in press.
18. Naito, M., Hayashi, N., Hagiwara, H., Hiramatsu, N., Kasahara, A., Fusamoto, H. and Kamada, T. (1994) Serum hepatitis C virus RNA quantity and histological features of hepatitis C virus carriers with persistently normal ALT levels. Hepatology 19, 871-875.
19. Nakabayashi, H., Taketa, K., Miyano, K., Yamane, T. and Sato, J. (1982) Growth of human hepatoma cell lines with differentiated functions in chemically defined medium. Cancer Res. 42, 3858-3863.
20. Santolini, E., Migliacco, G. and La Monica, N. (1994) Biosynthesis and biochemical properties of the hepatitis core protein. J. Virol. 68, 3631-3641.
21. Selby, M.J., Choo, Q.L., Berger, K., Kuo, G., Glazer, E., Eckart, M., Lee, C., Chien, D., Kuo, C. and Houghton, M. (1993) Expression, identification and subcellular localization of the protein encoded by the hepatitis C viral genome. J. Gen. Virol. 74, 1103-1113.
22. Shihi, C.M., Lo, S.J., Miyamura, T., Chen, S.Y. and Lee, Y.H.W. (1993) Suppression of hepatitis B virus expression and replication by hepatitis C virus core protein in HuH7-cells. J. Virol. 67, 5823-5832.
23. Uetsuki, T., Naito, A., Nagata, S. and Kaziro, Y. (1989) Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor 1-α. J. Biol. Chem. 264, 5791-5798.
24. Wakita, T. and Wands, J.R. (1994) Specific inhibition of hepatitis C virus expression by antisense oligodeoxynucleotides. J. Biol. Chem. 269, 14205-14210.
25. Wang, B., Merva, M., Dang, K., Ugen, K., Boyer, J., William, W. and Weiner, D.B. (1994) DNA inoculation induces protective in vivo immune responses against cellular challenge with HIV-1 antigen-expressing cells. AIDS Res. Hum. Retroviruses 10, S35-S41.
26. Zhu, N., Matsumoto, M., Hwang, S.B., Su, P.Y., Arsdale, T., Ware, C.F. and Lai, M.C. (1996) Hepatitis C virus core protein interacts with tumor necrosis factor receptors. Keystone Symposia, Hepatitis C and Beyond (B2), Abstract, 17.