Detection of fibronectin expression by human endothelial cells using an enzyme-linked immunosorbent assay (ELISA): Enzymatic degradation by activated plasminogen

Journal of Immunological Methods

Volumn 202, Issue 1, 1997, Pages 67-75

  Daramola, O A ^a   Heyderman, R S ^a   Klein, N J ^b   Shennan, G I ^a   Levin, M ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

endothelium; fibronectin; immunoassay; plasminogen

Indexed keywords

FIBRONECTIN; MONOCLONAL ANTIBODY; PLASMINOGEN;

ARTICLE; ENDOTHELIUM CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; NEWBORN; NORMAL HUMAN; PRIORITY JOURNAL; UMBILICAL VEIN;

ANTIBODIES, MONOCLONAL; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; ENZYME ACTIVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBRONECTINS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; PLASMINOGEN; STAINING AND LABELING; UMBILICAL VEINS;

MURINAE;

EID: 0031052471     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00237-2     Document Type: Article

References (35)

1. Atherton, B.T. and Hynes, R.O. (1981) A difference between plasma and cellular fibronectins located with monoclonal anti-bodies. Cell 25, 133.
2. Bishop, G.A. and Hwang, J. (1992) Use of a cellular ELISA for the detection of cell surface antigens. Biotechniques 12, 326.
3. Couchman, J.R., Austria, M.R. and Woods, A. (1990) Fibronectin-cell interactions. J. Invest. Dermatol. 94, 7.
4. Danø, K., Andreasen, P.A., Grøndahl-Hansen, J., Kristensen, P., Nielsen, L.S. and Skriver, L. (1985) Plasminogen activators, tissue degradation and cancer. Adv. Cancer Res. 44, 139.
5. Danon, D. and Shutelsky, E. (1976) Endothelial surface charge and its possible relationship to thrombogenesis. Ann. NY Acad. Sci. 275, 47.
6. Forsyth, K.D. and Levinsky, R.J. (1990) Fibronectin degradation; an in-vitro model of neutrophil mediated endothelial cell damage. J. Pathol. 161, 313.
7. Friberger, P., Knös, M., Gustavsson, S., Aurell, L. and Claeson, G. (1978) Methods for determination of plasmin, antiplasmin and plasminogen by means of substrate S-2251. Haemostasis 7, 138.
8. Gimbrone, M.A. (1986) Vascular endothelium: nature's blood container. In: M.A. Gimbrone (Eds.), Endothelium in Hemostasis and Thrombosis. Churchill Livingstone, Edinburgh, p. 1.
9. Gutman, A. and Kornblihtt, A.R. (1987) Identification of a third region of cell-specfic alternative splicing in a human fibronectin mRNA. Proc. Natl. Acad. Sci. USA 84, 7179.
10. Hajjar, K.A., Harpel, P.C., Jaffe, E.A. and Nachman, R.L. (1986) Binding of plasminogen to cultured human endothelial cells. J. Biol. Chem. 261, 11656.
11. Hantai, D. and Festoff, B.W. (1987) Degradation of muscle basement membrane by locally generated plasmin. Exp. Neurol. 95, 44.
12. Hynes, R. (1982) Fibronection and its relation to cellular structure and behavior. In: E.D. Hay (Eds.), Cell Biology of Extracellular Matrix. Press Publishing Co., New York, p. 295.
13. Hynes, R.O. and Yamada, K.M. (1982) Fibronectins: Multifunctional Modular Glycoproteins. J. Cell Biol. 95, 369.
14. Jaffe, E.A., Nachman, R.L., Becker, C.G. and Minick, C.R. (1973) Culture of human endothelial cells derived from umbilical veins. J. Clin. Invest. 52, 2745.
15. Karlsson, K. and Marklund, S.L. (1988) Plasma clearance of extracellular - superoxide dismutase c in rabbits. J. Clin. Invest. 82, 762.
16. Klein, N.J., Shennan, G.I., Heyderman, R.S. and Levin, M. (1992) Alteration in glycosaminoglycan metabolism and surface charge on human umbilical vein endothelial cells induced by cytokines, endotoxin and neutrophils. J. Cell Sci. 102, 821.
17. Klein, N.J., Levin, M., Strobel, S. and Finn, A. (1993a) Degradation of glycosaminoglycans and fibronectin on endotoxin-stimulated endothelium by adherent neutrophils: Relationship to CD11b/CD18 and L-selectin expression. J. Infect. Dis. 167, 890.
18. Klein, N.J., Shennan, G.I., Heyderman, R.S. and Levin, M. (1993b) Detection of glycosaminoglycans on the surface of human umbilical vien endothelial cells using gold-conjugated poly-L-lysine with silver enhancement. Histochem. J. 25, 291.
19. Klotz, S.A. and Maca, R.D. (1988) Endothelial cell contraction increases Candida adherence to exposed extracellular matrix. Infect. Immun. 56, 2495.
20. Kuusela, P., Ullberg, M., Saksela, O. and Kronvall, G. (1992) Tissue-type plasminogen activator-mediated activation of plasminogen on the surface of group A, C, and G streptococci. Infect. Immunol. 60, 196.
21. Labat-Robert, J., Bihari-Varga, M. and Robert, L. (1990) Extracellular matrix. FEBS Lett. 268, 386.
22. Levin, M. (1990) The inflammatory response to infections. In: J. de Louvois and D. Harvey (Eds.), Infection in the Newborn. John Wiley & Sons Ltd., New York, p. 1.
23. Liotta, L.A., Lee, C. and Morakis, D.J. (1980) New method for preparing whole intact surfaces of human basement membrane for tumor invasion studies. Cancer Lett. 11, 141.
24. Liotta, L.A., Goldfarb, R.H., Brundage, R., Siegal, G.P., Terranova, V. and Garbisa, S. (1981) Effect of plasminogen activator (urokinase), plasmin, and thrombin on glycoprotein and collagenous components of basement membrane. Cancer Res. 41, 4629.
25. Mosher, D.F. (1984) Physiology of fibronection. Annu. Rev. Med. 35, 561.
26. Odenthal, M., Neubauer, K., Baralle, F.E., Peters, H., Meyer Zum Büschenfelde, K.H. and Ramadori, G. (1992) Rat hepatocytes in primary culture synthesize and secrete cellular fibronectin. Exp. Cell Res. 203, 289.
27. Pierschbacher, M.D., Hayman, E.G. and Ruoslahti, E. (1981) Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule. Cell 26, 259.
28. Pöllänen, J., Stephens, R.W. and Vaheri, A. (1991) Directed plasminogen activation at the surface of normal and malignant cells. Adv. Cancer Res. 57, 273.
29. Ruoslahti, E. (1988) Fibronectin and its receptors. Annu. Rev. Biochem. 57, 375.
30. Ruoslahti, E. and Pierschbacher, M.D. (1986) Arg-Gly-Asp: A versatile cell recognition signal. Cell 44, 517.
31. Salonen, E., Saksela, O., Vartio, T., Vaheri, A., Nielsen, L.S. and Zeuthen, J. (1985) Plasminogen and tissue-type plasminogen activator bind to immobilized fibronectin. J. Biol. Chem. 260, 12302.
32. Vassalli, J., Sappino, A. and Belin, D. (1991) The plasminogen activator/plasmin system. J. Clin. Invest. 88, 1067.
33. Yamada, K.M. (1982) In: H. Furthmayr (Eds.), Immunocytochemistry of the Extracellular Matrix. CRC Boca Raton, FL, p. 111.
34. Yamada, K.M. and Kennedy, D.W. (1979) Fibroblast cellular and plasma fibronectins are similar but not identical. J. Cell Biol. 80, 492.
35. Ylätupa, S., Partanen, P., Haglund, C. and Virtanen, I. (1993) Competitive enzyme immunoassay for quantification of the cellular form of fibronectin (EDAcFN) in blood samples. J. Immunol. Methods. 163, 41.