Quantitation of ethidium-stained closed circular DNA in agarose gels

Journal of Biochemical and Biophysical Methods

Volumn 34, Issue 1, 1997, Pages 73-79

  Shubsda, Michael F ^a   Goodisman, Jerry ^a   Dabrowiak, James C ^a  

^a SYRACUSE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CIRCULAR DNA; ETHIDIUM BROMIDE;

ACCURACY; ANALYTICAL ERROR; ARTICLE; DNA DETERMINATION; ELECTROPHORESIS; FLUORESCENCE; MICRODENSITOMETRY; PHOTOGRAPHY; PRIORITY JOURNAL; QUANTITATIVE ASSAY; TECHNIQUE;

BACTERIOPHAGES; DENSITOMETRY; DNA, CIRCULAR; DNA, SUPERHELICAL; DNA, VIRAL; ELECTROPHORESIS, AGAR GEL; ETHIDIUM; EVALUATION STUDIES; FLUORESCENT DYES; PLASMIDS; SIMIAN VIRUS 40;

EID: 0031052217     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(96)01204-3     Document Type: Article

References (12)

1. Gale, E.F., Cundliffe, E., Reynolds, P.E., Richmond, M.H. and Waring, M.J., The molecular basis for antibiotic action, John Wiley and Sons, New York, 1981.
2. Lilley, D.M.J., DNA: Sequence structure and supercoiling, Biochem. Soc. Trans., 12 (1984) 127-140.
3. Maniatis, T., Fritsch, E.F. and Sambrook, J., Molecular cloning: a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York, 1989.
4. Prunell, A., Strauss, F. and Leblanc, B., Photographic quantitation of DNA in gel electrophoresis, Anal. Biochem., 78 (1977) 57-65.
5. Ribeiro, E.A., Larcom and Miller, D.P., Quantitative fluorescence of DNA-intercalated ethidium bromide on agarose gels, Anal. Biochem., 181 (1989) 197-208.
6. LePecq, J.-B. and Paoletti, C., A fluorescent complex between ethidium bromide and nucleic acids, J. Mol. Biol., 27 (1967) 87-106.
7. Bauer, W. and Vinograd, J., The interaction of closed circular DNA with intercalative dyes, J. Mol. Biol., 33 (1968) 141-171.
8. 3 satellite DNA from calf thymus, Eur. J. Biochem., 89 (1978) 267-279.
9. McMurray, C.T., Small, E.W. and van Holde, K.E., Binding of ethidium to the nucleosome core particle. 2. Internal and external binding modes, Biochemistry, 30 (1991) 5644-5652.
10. Löbrich, M., Ikpeme, S., Haub, P., Weber, K.J. and Kiefer, J., DNA double-strand break induction in yeast by x-rays and α-particles measured by pulsed-field gel electrophoresis, Int. J. Radiat. Biol., 64 (1993) 539-546.
11. Kishikawa, H., Jiang, Y.-P., Goodisman, J. and Dabrowiak, J.C., Coupled kinetic analysis of cleavage of DNA by esperamicin and calicheamicin, J. Am. Chem. Soc., 113 (1991) 5434-5440.
12. Sehlstedt, U., Kim, S.K., Carter, P., Goodisman, J., Vollano, J.F., Nordén, B. and Dabrowiak, J.C., Interaction of cationic porphyrins with DNA, Biochemistry, 33 (1994) 417-426.