Immunocytochemical phenotyping of disseminated tumor cells in bone marrow by uPA receptor and CK18: Investigation of sensitivity and specificity of an immunogold/alkaline phosphatase double staining protocol

Journal of Histochemistry and Cytochemistry

Volumn 45, Issue 2, 1997, Pages 203-212

  Allgayer, Heike ^a   Heiss, Markus Maria ^a   Riesenberg, Rainer ^a   Babic, Rudolf ^b   Jauch, Karl Walter ^a   Schildberg, Friedrich Wilhelm ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b Institute of Pathology and Cytology   (Germany)

Author keywords

bone marrow; CK18 positive cells; immunogold APAAP double staining; methodological aspects; phenotyping; uPA receptor

Indexed keywords

ALKALINE PHOSPHATASE; CYTOKERATIN; TUMOR MARKER; UROKINASE;

ARTICLE; BONE MARROW; CANCER SCREENING; CELLULAR DISTRIBUTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD STAINING; MAJOR CLINICAL STUDY; PHENOTYPE; PRIORITY JOURNAL; STOMACH CANCER; TUMOR CELL;

EID: 0031050286     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549704500206     Document Type: Article

References (38)

1. Antica M, Heiss MM, Kummer U, Munker R, Thiel E, Thierfelder S (1986) Simultaneous demonstration of two antigens on single T cells using antibodies with contrasting labels. J Immunol Methods 87:129-136
2. Bastholm L, Nielsen MH, DeMey J, Dano K, Brunner N, Hoyer-Hansen G, Ronne E, Elling FAD (1994) Confocal fluorescence microscopy of urokinase plasminogen activator receptor and cathepsin D in human MDA-MB-231 breast cancer cells migrating in reconstituted basement membrane. Biotech Histochem 69: 61-67
3. Berger U, Bettelheim R, Mansi JL, Easton D, Coombes RC, Neville AM (1988) The relationship between micrometastasis in the bone marrow, histopathologic features of the primary tumor in breast cancer and prognosis. Am J Clin Pathol 90:1-6
4. Blasi F (1993) Urokinase and urokinase receptor: a paracrine/autocrine system regulating cell migration and invasiveness. BioEssays 15:105-111
5. Blatt WF, Robinson SM (1968) Membrane ultrafiltration: the diafiltration technique and its application to microsolute exchange and binding phenomena. Anal Biochem 26:151-173
6. Boenisch T (1989) Hintergrundreaktivität. In Naish SJ, ed. Handbuch Immunchemischer Färbemethoden II. Carpinteria, CA, Dako Corporation, 30-33
7. Bonnard C, Papermaster DS, Kraehenbuhl JP (1984) The streptavidin-biotin bridge technique: application in light and electron microscope immunocytochemistry. In Polak JM, Varndell IM, eds. Immunolabelling for Electron Microscopy. Amsterdam, Elsevier, 95-111
8. Chucholowski N, Schmitt M, Rettenberger P, Schüren E, Moniwa N, Groetzki L, Wilhelm O, Weildle U, Jänicke F, Graeff H (1992) Flow cytofluorometric analysis of the urokinase receptor (uPAR) on tumor cells by fluorescent uPA-ligand or monoclonal antibody 3936. Fibrinolysis 6(suppl 4):95-102
9. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219-229
10. Danscher G (1981) Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71:81-88
11. De Valck V, Renmans W, Segers E, Leunissen J, De Waele M (1991) Light microscopical detection of leucocyte cell surface antigens with a one-nanometer gold probe. Histochemistry 95:483-490
12. Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710-1715
13. Gu J, De Mey J, Moeremans M, Polak JM (1981) Sequential use of the PAP and immunogold staining methods for the light microscopical double staining of tissue antigens: first application to the study of regulatory peptides in the gut. Regul Pept 1:365-375
14. Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131-1139
15. Heiss MM, Babic R, Allgayer H, Grützner KU, Jauch KW, Löhrs U, Schildberg FW (1995b) Tumor-associated proteolysis and prognosis: new functional risk factors in gastric cancer defined by the urokinase-type plasminogen activator system. J Clin Oncol 13: 2084-2093
16. Heiss MM, Allgayer H, Gruetzner KU, Funke I, Babic R, Jauch KW, Schildberg FW (1995a) Individual develpoment and uPA-R-expression of disseminated tumor cells in bone marrow: a reference to early systemic disease in solid cancer. Nature Medicine 1:1035-1039
17. Holgate CS, Jackson P, Cowen PN, Bird CC (1983) Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31:938-944
18. Jankun J, Merrick HW, Goldblatt PJ (1993) Expression and localization of elements of the plasminogen activation system in benign breast disease and breast cancers. J Cell Biochem 53:135-144
19. Jauch KW, Heiss MM, Grützner KU, Funke I, Pantel K, Babic R, Eissner HJ, Riethmueller G, Schildberg FW (1996) The prognostic significance of early disseminated tumor cells in bone marrow of patients with gastric cancer. J Clin Oncol, in press
20. Kramer MD, Spring H, Todd RF, Vettel UAD (1994) Urokinase-type plasminogen activator enhances invasion of human T-cells (Jurkat) into a fibrin matrix. J Leukocyte Biol 56:110-116
21. Lindemann F, Schlimok G, Dirschedl P, Witte J, Riethmüller G (1992) Prognostic significance of micrometastatic tumor cells in bone marrow of colorectal cancer patients. Lancet 340:685-689
22. Loos JA, Roos D (1974) Ficoll-Isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticuloendothelial cells. Exp Cell Res 86:333-341
23. Mason DY, Woolston RE (1982) Double immunoenzymatic labelling. In Bullock G, Petrusz P, eds. Techniques in Immunocytochemistry. Vol 1. London, Academic Press, 135-152
24. Moll R, Franke WW, Schiller DL, Geiger B, Krepier R (1982) The catalogue of human cytokeratins: pattern of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24
25. Moller LB (1993) Structure and function of the urokinase receptor. Blood Coagulation Fibrinolysis 4:293-303
26. Moss TJ, Reynold CP, Sather HN, Romansky SG, Hammond GD, Seeger RC (1991) Prognostic value of immunocytologic detection of bone marrow metastases in neuroblastoma. N Engl J Med 324:219-226
27. Pantel K, Izbicki JR, Angstwurm M, Braun S, Passlick B, Karg O, Thetter O, Riethmüller G (1993a) Immunocytological detection of bone marrow micrometastasis in operable non-small cell lung cancer. Cancer Res 53:1027-1031
28. Pantel K, Schlimok G, Angstwurm M, Weckermann D, Schmaus W, Gath H, Passlick B, Izbicki JR, Riethmüller GAD (1994) Methodological analysis of immunocytochemical screening for disseminated epithelial tumor cells in bone marrow. J Hematother 3: 165-173
29. Pantel K, Schlimok G, Braun S, Kutter D, Lindemann F, Schaller G, Funke I, Izbicki J, Riethmüller G (1993b) Differential expression of proliferation-associated molecules in individual micrometastatic carcinoma cells. J Natl Cancer Inst 85:1419-1423
30. Plesner T, Ralfkiaer E, Wittrup M, Johnsen H, Pyke C, Pedersen TL, Hansen NE, Dano KAD (1994) Expression of the receptor for urokinase-type plasminogen activator in normal and neoplastic blood cells and hematopoetic tissue. Am J Clin Pathol 102: 835-841
31. Rathlev T, Hocko JM, Franks GF, Suffin SC, O'Donnell CM, Porter DD (1981) Glucose oxidase immunoenzyme methodology as a substitute for fluorescence microscopy in the clinical laboratory. Clin Chem 27:1513-1515
32. Riesenberg R, Oberneder R, Kriegmair M, Epp M, Bitzer U, Hofstetter A, Braun S, Riethmüller G, Pantel K (1993) Immunocytochemical double staining of cytokeratin and prostate specific antigen in individual prostatic tumour cells. Histochemistry 99: 61-66
33. Sako H, Nakane Y, Okino K, Nishihara K, Kodama M, Kawata M, Yamada H (1986) Simultaneous detection of B-cells and T-cells by a double immunohistochemical technique using immunogold-silver staining and the avidin-biotin-peroxidase complex method. Histochemistry 86:1-4
34. Schlimok G, Funke I, Holzmann B, Göttlinger G, Schmidt G, Häuser H, Swierkot S, Warnecke HH, Schneider B, Koprowski H, Riethmüller G (1987) Micrometastatic cancer cells in bone marrow: in vitro detection with anti-cytokeratin and in vivo labeling with anti-17-1-A monoclonal antibodies. Proc Natl Acad Sci 84:8672-8676
35. Scopsi L, Larsson LI (1985) Increased sensitivity in immunocytochemistry. Effects of double application of antibodies and of silver intensification on immunogold and peroxidase-antiperoxidase staining techniques. Histochemistry 82:321-329
36. Shapiro HM (1995) Practical Flow Cytometry. 3rd ed. New York, Chichester, Brisbane, Toronto, Singapore, Wiley-Liss
37. Van den Pol AN (1984) Colloidal gold and biotin-avidin conjugates as ultrastructural markers for neural antigens. Q J Exp Physiol 69:1-33
38. Wilson EL, Jacobs P, Dowdle EB (1983) The secretion of plasminogen activators by human myeloid leukemic cells in vitro. Blood 61:568-574